DOI: 10.1128/jvi.72.5.4498-4502.1998. Respiratory Syncytial Virus Infection of Human Alveolar Epithelial Cells Enhances Interferon Regulatory Factor 1 and Interleukin-1β-Converting Enzyme Gene Expression but Does Not Cause Apoptosis

Respiratory Syncytial Virus Infection of Human Alveolar Epithelial Cells Enhances Interferon Regulatory Factor 1 and Interleukin-1β-Converting Enzyme Gene Expression but Does Not Cause Apoptosis

10.1128/jvi.72.5.4498-4502.1998

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT The induction kinetics of the transcriptional activities of interferon regulatory factor 1 (IRF-1), interleukin-1β-converting enzyme (ICE), and CPP32 by respiratory syncytial virus (RSV) infection of human type II alveolar epithelial cells (A549 cells) were analyzed semiquantitatively by reverse transcriptase PCR. The appearance of ICE and CPP32 protein in cell lysate was examined by Western blotting analysis. The induction of apoptosis by RSV infection was examined by the appearance of DNA fragmentation detected by terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling. RSV moderately enhanced IRF-1 mRNA as early as 4 h after infection, and this enhancement lasted several hours. Following induction of the IRF-1 gene, ICE gene expression increased significantly, and an increase of ICE protein was observed in the RSV-infected cell lysate. These increments were observed in cells treated with live RSV but not in cells treated with inactivated RSV or control antigen. However, no infection-specific increase of CPP32 gene expression or the protein was observed. No nucleosomal fragmentation was observed in RSV-infected cells during the whole course of infection, despite the appearance of extensive cytopathic change and cell death. These observations suggest that RSV infection of human alveolar epithelial cells induces the ICE gene and its protein as a result of increased IRF-1 induction but that the increased ICE was insufficient to cause apoptosis in the RSV-infected cells. ICE might not be able to activate CPP32, which is thought to be the more important protease for apoptosis.

Bibliography

Takeuchi, R., Tsutsumi, H., Osaki, M., Haseyama, K., Mizue, N., & Chiba, S. (1998). Respiratory Syncytial Virus Infection of Human Alveolar Epithelial Cells Enhances Interferon Regulatory Factor 1 and Interleukin-1Î²-Converting Enzyme Gene Expression but Does Not Cause Apoptosis. Journal of Virology, 72(5), 4498â4502.

Authors 6

Ryoh Takeuchi (first)
Hiroyuki Tsutsumi (additional)
Masaya Osaki (additional)
Keiji Haseyama (additional)
Nobuo Mizue (additional)
Shunzo Chiba (additional)

References 37 Referenced 37

Collins P. L. McIntosh K. Chanock R. M. Respiratory syncytial virus Fields virology 3rd ed. Fields B. N. Knipe D. M. 1996 1313 1351 Raven Press New York N.Y
10.1016/S0022-3476(86)81034-4
Duke R. C. Apoptosis in cell-mediated immunity Apoptosis: the molecular basis of cell death. Tomei L. D. Cope F. O. 1991 209 226 Cold Spring Harbor Laboratory Cold Spring Harbor N.Y
10.1016/0167-5699(86)90152-0
10.1038/380723a0
10.1002/j.1460-2075.1995.tb07183.x
10.1128/jvi.70.12.9079-9082.1996
10.1083/jcb.119.3.493
10.1126/science.8098552
Hasegawa J. Kamada S. Kamiike W. Shimizu S. Imazu T. Matsuda H. Tsujimoto Y. Involvement of CPP32/Yama(-like) proteases in Fas-mediated apoptosis.Cancer Res. 56 1996 1713 1718 / Cancer Res. / Involvement of CPP32/Yama(-like) proteases in Fas-mediated apoptosis by Hasegawa J. (1996)
10.1128/jvi.68.6.3667-3673.1994
10.1056/NEJM197006042822302
10.1016/0092-8674(91)90614-5
Kerr J. F. R. Harmon B. V. Definition and incidence of apoptosis: a historical perspective Apoptosis: the molecular basis of cell death. Tomei L. D. Cope F. O. 1991 5 29 Cold Spring Harbor Laboratory Cold Spring Harbor N.Y
10.1093/nar/17.8.3292
10.1128/cdli.2.3.322-324.1995
10.1002/(SICI)1096-9071(199602)48:2<199::AID-JMV13>3.0.CO;2-A
10.1016/S0092-8674(88)91307-4
10.1038/376037a0
10.1093/infdis/171.3.584
10.1165/ajrcmb.13.5.7576697
10.1128/JVI.72.1.837-840.1998
Takizawa T. Induction of apoptosis by influenza virus infection.Virus 47 1997 69 76 (In Japanese.) / Virus / Induction of apoptosis by influenza virus infection by Takizawa T. (1997)
10.1006/viro.1995.1260
10.1099/0022-1317-74-11-2347
10.1038/376596a0
10.1006/bbrc.1996.1752
10.1016/0092-8674(94)90132-5
10.1172/JCI115188
10.1074/jbc.270.7.3255
10.1016/0092-8674(95)90541-3
10.1038/356768a0
10.1099/0022-1317-68-8-2161
10.1126/science.274.5288.787
10.1126/science.274.5288.784
10.1016/S0074-7696(08)62312-8
10.1084/jem.169.5.1747

Dates

Type	When
Created	5 years, 7 months ago (Dec. 31, 2019, 1:13 p.m.)
Deposited	3 years, 5 months ago (March 4, 2022, 9:55 p.m.)
Indexed	4 months, 1 week ago (April 19, 2025, 12:16 a.m.)
Issued	27 years, 3 months ago (May 1, 1998)
Published	27 years, 3 months ago (May 1, 1998)
Published Print	27 years, 3 months ago (May 1, 1998)

Funders 0

None

BibTeX

@article{Takeuchi_1998, title={Respiratory Syncytial Virus Infection of Human Alveolar Epithelial Cells Enhances Interferon Regulatory Factor 1 and Interleukin-1β-Converting Enzyme Gene Expression but Does Not Cause Apoptosis}, volume={72}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.72.5.4498-4502.1998}, DOI={10.1128/jvi.72.5.4498-4502.1998}, number={5}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Takeuchi, Ryoh and Tsutsumi, Hiroyuki and Osaki, Masaya and Haseyama, Keiji and Mizue, Nobuo and Chiba, Shunzo}, year={1998}, month=may, pages={4498–4502} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        4,
        19
      ]
    ],
    "date-time": "2025-04-19T04:16:15Z",
    "timestamp": 1745036175333
  },
  "reference-count": 37,
  "publisher": "American Society for Microbiology",
  "issue": "5",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1998,
            5,
            1
          ]
        ],
        "date-time": "1998-05-01T00:00:00Z",
        "timestamp": 893980800000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://journals.asm.org/non-commercial-tdm-license"
    }
  ],
  "content-domain": {
    "domain": [
      "journals.asm.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1998,
        5
      ]
    ]
  },
  "abstract": "<jats:title>ABSTRACT</jats:title>\n          <jats:p>The induction kinetics of the transcriptional activities of interferon regulatory factor 1 (IRF-1), interleukin-1\u03b2-converting enzyme (ICE), and CPP32 by respiratory syncytial virus (RSV) infection of human type II alveolar epithelial cells (A549 cells) were analyzed semiquantitatively by reverse transcriptase PCR. The appearance of ICE and CPP32 protein in cell lysate was examined by Western blotting analysis. The induction of apoptosis by RSV infection was examined by the appearance of DNA fragmentation detected by terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling. RSV moderately enhanced IRF-1 mRNA as early as 4 h after infection, and this enhancement lasted several hours. Following induction of the IRF-1 gene, ICE gene expression increased significantly, and an increase of ICE protein was observed in the RSV-infected cell lysate. These increments were observed in cells treated with live RSV but not in cells treated with inactivated RSV or control antigen. However, no infection-specific increase of CPP32 gene expression or the protein was observed. No nucleosomal fragmentation was observed in RSV-infected cells during the whole course of infection, despite the appearance of extensive cytopathic change and cell death. These observations suggest that RSV infection of human alveolar epithelial cells induces the ICE gene and its protein as a result of increased IRF-1 induction but that the increased ICE was insufficient to cause apoptosis in the RSV-infected cells. ICE might not be able to activate CPP32, which is thought to be the more important protease for apoptosis.</jats:p>",
  "DOI": "10.1128/jvi.72.5.4498-4502.1998",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2019,
        12,
        31
      ]
    ],
    "date-time": "2019-12-31T18:13:11Z",
    "timestamp": 1577815991000
  },
  "page": "4498-4502",
  "update-policy": "http://dx.doi.org/10.1128/asmj-crossmark-policy-page",
  "source": "Crossref",
  "is-referenced-by-count": 37,
  "title": "Respiratory Syncytial Virus Infection of Human Alveolar Epithelial Cells Enhances Interferon Regulatory Factor 1 and Interleukin-1\u03b2-Converting Enzyme Gene Expression but Does Not Cause Apoptosis",
  "prefix": "10.1128",
  "volume": "72",
  "author": [
    {
      "given": "Ryoh",
      "family": "Takeuchi",
      "sequence": "first",
      "affiliation": [
        {
          "name": "<!--label omitted: 1-->Department of Pediatrics, Sapporo Medical University School of Medicine, Sapporo, Japan"
        }
      ]
    },
    {
      "given": "Hiroyuki",
      "family": "Tsutsumi",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "<!--label omitted: 1-->Department of Pediatrics, Sapporo Medical University School of Medicine, Sapporo, Japan"
        }
      ]
    },
    {
      "given": "Masaya",
      "family": "Osaki",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "<!--label omitted: 1-->Department of Pediatrics, Sapporo Medical University School of Medicine, Sapporo, Japan"
        }
      ]
    },
    {
      "given": "Keiji",
      "family": "Haseyama",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "<!--label omitted: 1-->Department of Pediatrics, Sapporo Medical University School of Medicine, Sapporo, Japan"
        }
      ]
    },
    {
      "given": "Nobuo",
      "family": "Mizue",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "<!--label omitted: 1-->Department of Pediatrics, Sapporo Medical University School of Medicine, Sapporo, Japan"
        }
      ]
    },
    {
      "given": "Shunzo",
      "family": "Chiba",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "<!--label omitted: 1-->Department of Pediatrics, Sapporo Medical University School of Medicine, Sapporo, Japan"
        }
      ]
    }
  ],
  "member": "235",
  "reference": [
    {
      "key": "e_1_3_1_2_2",
      "unstructured": "Collins\nP. L.\nMcIntosh\nK.\nChanock\nR. M.\nRespiratory syncytial virus\nFields virology\n3rd ed.\nFields\nB. N.\nKnipe\nD. M.\n1996\n1313\n1351\nRaven Press\nNew York N.Y"
    },
    {
      "key": "e_1_3_1_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0022-3476(86)81034-4"
    },
    {
      "key": "e_1_3_1_4_2",
      "unstructured": "Duke\nR. C.\nApoptosis in cell-mediated immunity\nApoptosis: the molecular basis of cell death.\nTomei\nL. D.\nCope\nF. O.\n1991\n209\n226\nCold Spring Harbor Laboratory\nCold Spring Harbor N.Y"
    },
    {
      "key": "e_1_3_1_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0167-5699(86)90152-0"
    },
    {
      "key": "e_1_3_1_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/380723a0"
    },
    {
      "key": "e_1_3_1_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1995.tb07183.x"
    },
    {
      "key": "e_1_3_1_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.70.12.9079-9082.1996"
    },
    {
      "key": "e_1_3_1_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.119.3.493"
    },
    {
      "key": "e_1_3_1_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.8098552"
    },
    {
      "key": "e_1_3_1_11_2",
      "first-page": "1713",
      "article-title": "Involvement of CPP32/Yama(-like) proteases in Fas-mediated apoptosis",
      "volume": "56",
      "author": "Hasegawa J.",
      "year": "1996",
      "unstructured": "Hasegawa J. Kamada S. Kamiike W. Shimizu S. Imazu T. Matsuda H. Tsujimoto Y. Involvement of CPP32/Yama(-like) proteases in Fas-mediated apoptosis.Cancer Res. 56 1996 1713 1718",
      "journal-title": "Cancer Res."
    },
    {
      "key": "e_1_3_1_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.68.6.3667-3673.1994"
    },
    {
      "key": "e_1_3_1_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1056/NEJM197006042822302"
    },
    {
      "key": "e_1_3_1_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(91)90614-5"
    },
    {
      "key": "e_1_3_1_15_2",
      "unstructured": "Kerr\nJ. F. R.\nHarmon\nB. V.\nDefinition and incidence of apoptosis: a historical perspective\nApoptosis: the molecular basis of cell death.\nTomei\nL. D.\nCope\nF. O.\n1991\n5\n29\nCold Spring Harbor Laboratory\nCold Spring Harbor N.Y"
    },
    {
      "key": "e_1_3_1_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/17.8.3292"
    },
    {
      "key": "e_1_3_1_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/cdli.2.3.322-324.1995"
    },
    {
      "key": "e_1_3_1_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/(SICI)1096-9071(199602)48:2<199::AID-JMV13>3.0.CO;2-A"
    },
    {
      "key": "e_1_3_1_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(88)91307-4"
    },
    {
      "key": "e_1_3_1_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/376037a0"
    },
    {
      "key": "e_1_3_1_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/infdis/171.3.584"
    },
    {
      "key": "e_1_3_1_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1165/ajrcmb.13.5.7576697"
    },
    {
      "key": "e_1_3_1_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.72.1.837-840.1998"
    },
    {
      "key": "e_1_3_1_24_2",
      "first-page": "69",
      "article-title": "Induction of apoptosis by influenza virus infection",
      "volume": "47",
      "author": "Takizawa T.",
      "year": "1997",
      "unstructured": "Takizawa T. Induction of apoptosis by influenza virus infection.Virus 47 1997 69 76 (In Japanese.)",
      "journal-title": "Virus"
    },
    {
      "key": "e_1_3_1_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/viro.1995.1260"
    },
    {
      "key": "e_1_3_1_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1099/0022-1317-74-11-2347"
    },
    {
      "key": "e_1_3_1_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/376596a0"
    },
    {
      "key": "e_1_3_1_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/bbrc.1996.1752"
    },
    {
      "key": "e_1_3_1_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(94)90132-5"
    },
    {
      "key": "e_1_3_1_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1172/JCI115188"
    },
    {
      "key": "e_1_3_1_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.270.7.3255"
    },
    {
      "key": "e_1_3_1_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(95)90541-3"
    },
    {
      "key": "e_1_3_1_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/356768a0"
    },
    {
      "key": "e_1_3_1_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1099/0022-1317-68-8-2161"
    },
    {
      "key": "e_1_3_1_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.274.5288.787"
    },
    {
      "key": "e_1_3_1_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.274.5288.784"
    },
    {
      "key": "e_1_3_1_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0074-7696(08)62312-8"
    },
    {
      "key": "e_1_3_1_38_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1084/jem.169.5.1747"
    }
  ],
  "container-title": "Journal of Virology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/JVI.72.5.4498-4502.1998",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/JVI.72.5.4498-4502.1998",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        3,
        5
      ]
    ],
    "date-time": "2022-03-05T02:55:39Z",
    "timestamp": 1646448939000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.asm.org/doi/10.1128/JVI.72.5.4498-4502.1998"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1998,
        5
      ]
    ]
  },
  "references-count": 37,
  "journal-issue": {
    "issue": "5",
    "published-print": {
      "date-parts": [
        [
          1998,
          5
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1128/JVI.72.5.4498-4502.1998"
  ],
  "URL": "http://dx.doi.org/10.1128/JVI.72.5.4498-4502.1998",
  "relation": {},
  "ISSN": [
    "0022-538X",
    "1098-5514"
  ],
  "subject": [],
  "container-title-short": "J Virol",
  "published": {
    "date-parts": [
      [
        1998,
        5
      ]
    ]
  },
  "assertion": [
    {
      "value": "1997-10-27",
      "order": 0,
      "name": "received",
      "label": "Received",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "1998-01-28",
      "order": 1,
      "name": "accepted",
      "label": "Accepted",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "1998-05-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}