DOI: 10.1128/jvi.72.11.8669-8675.1998. Interactions of Soluble Recombinant Integrin αvβ5 with Human Adenoviruses

Interactions of Soluble Recombinant Integrin αvβ5 with Human Adenoviruses

10.1128/jvi.72.11.8669-8675.1998

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACTαv integrins have been identified as coreceptors for adenovirus (Ad) internalization; however, direct interactions of these molecules with Ad have not been demonstrated. We report here the expression of soluble integrin αvβ5, which retains the ability to recognize the Ad penton base as well as vitronectin, an Arg Gly Asp (RGD)-containing extracellular matrix protein. Soluble integrin αvβ5 reacted with seven different Ad serotypes (subgroups A to E) in solid-phase binding assays. The soluble integrin exhibited different levels of binding to each Ad serotype; however, binding to multiple Ad types required the presence of divalent metal cations and was inhibited by a synthetic RGD peptide, indicating that RGD and cation-binding sequences regulate Ad interactions with αvβ5. Incubation of Ad particles with soluble αvβ5 integrin also inhibited subsequent Ad internalization into epithelial cells as well as virus attachment to monocytic cells. These findings suggest that soluble αv integrins or antagonists of these coreceptors could be used to limit infection by multiple Ad types. The generation of soluble αv integrins should also permit further detailed kinetic and structural analysis of Ad interactions with its coreceptors.

Bibliography

Mathias, P., Galleno, M., & Nemerow, G. R. (1998). Interactions of Soluble Recombinant Integrin Î±vÎ²5 with Human Adenoviruses. Journal of Virology, 72(11), 8669â8675. LOCKSS.

Authors 3

Patricia Mathias (first)
Michael Galleno (additional)
Glen R. Nemerow (additional)

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Dates

Type	When
Created	5 years, 8 months ago (Dec. 31, 2019, 1:03 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 3:55 p.m.)
Indexed	4 months, 2 weeks ago (April 11, 2025, 1:29 a.m.)
Issued	26 years, 9 months ago (Nov. 1, 1998)
Published	26 years, 9 months ago (Nov. 1, 1998)
Published Print	26 years, 9 months ago (Nov. 1, 1998)

Funders 0

None

BibTeX

@article{Mathias_1998, title={Interactions of Soluble Recombinant Integrin αvβ5 with Human Adenoviruses}, volume={72}, ISSN={0022-538X}, url={http://dx.doi.org/10.1128/jvi.72.11.8669-8675.1998}, DOI={10.1128/jvi.72.11.8669-8675.1998}, number={11}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Mathias, Patricia and Galleno, Michael and Nemerow, Glen R.}, year={1998}, month=nov, pages={8669–8675} }

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