Crossref journal-article
American Society for Microbiology
Journal of Virology (235)
Abstract

The interaction of the human immunodeficiency virus type 1 (HIV-1) nucleoprotein complex with the cell nuclear import machinery is necessary for viral replication in macrophages and for the establishment of infection in quiescent T lymphocytes. The karyophilic properties of two viral proteins, matrix (MA) and Vpr, are keys to this process. Here, we show that an early step of HIV-1 nuclear import is the recognition of the MA nuclear localization signal (NLS) by Rch1, a member of the karyopherin-alpha family. Furthermore, we demonstrate that an N-terminally truncated form of Rch1 which binds MA but fails to localize to the nucleus efficiently blocks MA- but not Vpr-mediated HIV-1 nuclear import. Correspondingly, NLS peptide inhibits the nuclear migration of MA but not that of Vpr and prevents the infection of terminally differentiated macrophages by vpr-defective virus but not wild-type virus. These results are consistent with a model in which Rch1 or another member of the karyopherin-alpha family, through the recognition of the MA NLS, participates in docking the HIV-1 nucleoprotein complex at the nuclear pore. In addition, our data suggest that Vpr governs HIV-1 nuclear import through a distinct pathway.

Bibliography

Gallay, P., Stitt, V., Mundy, C., Oettinger, M., & Trono, D. (1996). Role of the karyopherin pathway in human immunodeficiency virus type 1 nuclear import. Journal of Virology, 70(2), 1027–1032.

Authors 5
  1. P Gallay (first)
  2. V Stitt (additional)
  3. C Mundy (additional)
  4. M Oettinger (additional)
  5. D Trono (additional)
References 0 Referenced 206

None

Dates
Type When
Created 5 years, 7 months ago (Jan. 6, 2020, 4:43 p.m.)
Deposited 3 years, 5 months ago (March 5, 2022, 8:16 a.m.)
Indexed 3 months, 1 week ago (May 18, 2025, 12:23 p.m.)
Issued 29 years, 6 months ago (Feb. 1, 1996)
Published 29 years, 6 months ago (Feb. 1, 1996)
Published Print 29 years, 6 months ago (Feb. 1, 1996)
Funders 0

None

@article{Gallay_1996, title={Role of the karyopherin pathway in human immunodeficiency virus type 1 nuclear import}, volume={70}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.70.2.1027-1032.1996}, DOI={10.1128/jvi.70.2.1027-1032.1996}, number={2}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Gallay, P and Stitt, V and Mundy, C and Oettinger, M and Trono, D}, year={1996}, month=feb, pages={1027–1032} }