DOI: 10.1128/jvi.44.2.674-682.1982. FBJ murine osteosarcoma virus: identification and molecular cloning of biologically active proviral DNA

FBJ murine osteosarcoma virus: identification and molecular cloning of biologically active proviral DNA

10.1128/jvi.44.2.674-682.1982

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

A 12.0-kilobase EcoRI restriction fragment containing FBJ murine osteosarcoma virus (FBJ-MSV) proviral DNA was identified in FBJ-MSV-transformed nonproducer rat cells and molecularly cloned in bacteriophage Charon 30 (lambda FBJ-1). A 5.8-kb HindIII fragment containing the entire FBJ-MSV proviral DNA was isolated from lambda FBJ-1 and subsequently subcloned in plasmid pBR322 (pFBJ-2). The DNA from recombinant plasmid pFBJ-2 was able to induce morphological transformation of rat fibroblasts in tissue culture. Transfected cells contained the p55 and p39 antigens specific for cells transformed by FBJ-MSV (T. Curran and N. M. Teich, J. Virol. 42:114-122, 1982). The organization of the FBJ-MSV provirus was analyzed by restriction endonuclease mapping, and a region of nonhomology with the helper virus was delineated. Sequences specific for this region (presumably the viral fos gene) were subcloned and used as a probe to identify related sequences present in the normal genomes of cells from a variety of mammalian species (cellular fos). A single-size (3.4 kilobases long) class of RNA hybridizing to the viral fos probe was identified in FBJ-MSV-transformed cells.

Bibliography

Curran, T., Peters, G., Van Beveren, C., Teich, N. M., & Verma, I. M. (1982). FBJ murine osteosarcoma virus: identification and molecular cloning of biologically active proviral DNA. Journal of Virology, 44(2), 674â682.

Authors 5

T Curran (first)
G Peters (additional)
C Van Beveren (additional)
N M Teich (additional)
I M Verma (additional)

References 0 Referenced 441

None

Dates

Type	When
Created	5 years, 7 months ago (Jan. 5, 2020, 8:08 p.m.)
Deposited	3 years, 5 months ago (March 4, 2022, 5:46 p.m.)
Indexed	1 month, 3 weeks ago (July 11, 2025, 6:29 a.m.)
Issued	42 years, 10 months ago (Nov. 1, 1982)
Published	42 years, 10 months ago (Nov. 1, 1982)
Published Print	42 years, 10 months ago (Nov. 1, 1982)

Funders 0

None

BibTeX

@article{Curran_1982, title={FBJ murine osteosarcoma virus: identification and molecular cloning of biologically active proviral DNA}, volume={44}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.44.2.674-682.1982}, DOI={10.1128/jvi.44.2.674-682.1982}, number={2}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Curran, T and Peters, G and Van Beveren, C and Teich, N M and Verma, I M}, year={1982}, month=nov, pages={674–682} }

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