DOI: 10.1128/jvi.32.1.72-77.1979. Inhibition of herpes simplex virus-induced DNA polymerase activity and viral DNA replication by 9-(2-hydroxyethoxymethyl)guanine and its triphosphate

Inhibition of herpes simplex virus-induced DNA polymerase activity and viral DNA replication by 9-(2-hydroxyethoxymethyl)guanine and its triphosphate

10.1128/jvi.32.1.72-77.1979

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

The effect of the nucleoside analog 9-(2-hydroxyethoxymethyl)guanine (acycloguanosine) on herpes simplex virus type 1 DNA synthesis was examined. Acycloguanosine inhibited herpesvirus DNA synthesis in virus-infected cells. The synthesis of host cell DNA was only partially inhibited in actively growing cells at acycloguanosine concentrations several hundred-fold greater than the 50% effective dose for herpes simplex virus type 1. Studies using partially purified enzymes revealed that the triphosphate of this compound inhibited the virus-induced DNA polymerases (DNA nucleotidyltransferases) to a greater degree than the DNA polymerase of the host cell, that the inhibition was dependent upon the base composition of the template, and that the triphosphate was a better substrate for the virus-induced polymerases than for the alpha cellular DNA polymerases.

Bibliography

Furman, P. A., St Clair, M. H., Fyfe, J. A., Rideout, J. L., Keller, P. M., & Elion, G. B. (1979). Inhibition of herpes simplex virus-induced DNA polymerase activity and viral DNA replication by 9-(2-hydroxyethoxymethyl)guanine and its triphosphate. Journal of Virology, 32(1), 72â77.

Authors 6

P A Furman (first)
M H St Clair (additional)
J A Fyfe (additional)
J L Rideout (additional)
P M Keller (additional)
G B Elion (additional)

References 0 Referenced 272

None

Dates

Type	When
Created	5 years, 8 months ago (Jan. 5, 2020, 7:49 p.m.)
Deposited	3 years, 6 months ago (March 4, 2022, 5:29 p.m.)
Indexed	2 days ago (Sept. 4, 2025, 9:17 a.m.)
Issued	45 years, 11 months ago (Oct. 1, 1979)
Published	45 years, 11 months ago (Oct. 1, 1979)
Published Print	45 years, 11 months ago (Oct. 1, 1979)

Funders 0

None

BibTeX

@article{Furman_1979, title={Inhibition of herpes simplex virus-induced DNA polymerase activity and viral DNA replication by 9-(2-hydroxyethoxymethyl)guanine and its triphosphate}, volume={32}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.32.1.72-77.1979}, DOI={10.1128/jvi.32.1.72-77.1979}, number={1}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Furman, P A and St Clair, M H and Fyfe, J A and Rideout, J L and Keller, P M and Elion, G B}, year={1979}, month=oct, pages={72–77} }

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