DOI: 10.1128/jvi.15.1.199-207.1975. Identification of the in vitro translation products of adenovirus mRNA by immunoprecipitation

Identification of the in vitro translation products of adenovirus mRNA by immunoprecipitation

10.1128/jvi.15.1.199-207.1975

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

Adenovirus type 2 mRNA was translated in S30 extracts from Ehrlich ascites and wheat embryo cells. The in vitro products were identified by sodium dodecyl sulfate-gel electrophoresis after immunoprecipitation with specific antisera in the presence of urea. Seven virion polypeptides could be identified by immunoprecipitation. Three of these appear to be precursors to polypeptides of the virion. mRNA isolated late in adenovirus infection was separated into three size classes by zonal sedimentation. Material sedimenting at 26S was translated into polypeptides corresponding to the largest virion polypeptides II to IV, a 22S fraction corresponding to polypeptide V, and smaller polypeptides and a 15S fraction corresponding to polypeptide IX. A significant amount of polypeptide IX was also synthesized by the 26S and 22S RNA.

Bibliography

Oberg, B., Saborio, J., Persson, T., Everitt, E., & Philipson, L. (1975). Identification of the in vitro translation products of adenovirus mRNA by immunoprecipitation. Journal of Virology, 15(1), 199â207.

Authors 5

B Oberg (first)
J Saborio (additional)
T Persson (additional)
E Everitt (additional)
L Philipson (additional)

References 0 Referenced 53

None

Dates

Type	When
Created	5 years, 7 months ago (Jan. 5, 2020, 7:23 p.m.)
Deposited	3 years, 5 months ago (March 4, 2022, 5:01 p.m.)
Indexed	4 weeks, 1 day ago (July 28, 2025, 5:42 p.m.)
Issued	50 years, 7 months ago (Jan. 1, 1975)
Published	50 years, 7 months ago (Jan. 1, 1975)
Published Print	50 years, 7 months ago (Jan. 1, 1975)

Funders 0

None

BibTeX

@article{Oberg_1975, title={Identification of the in vitro translation products of adenovirus mRNA by immunoprecipitation}, volume={15}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.15.1.199-207.1975}, DOI={10.1128/jvi.15.1.199-207.1975}, number={1}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Oberg, B and Saborio, J and Persson, T and Everitt, E and Philipson, L}, year={1975}, month=jan, pages={199–207} }

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