DOI: 10.1128/jvi.10.3.484-491.1972. Origin and Direction of Simian Virus 40 Deoxyribonucleic Acid Replication

Origin and Direction of Simian Virus 40 Deoxyribonucleic Acid Replication

10.1128/jvi.10.3.484-491.1972

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

Double-branched, circular, replicating deoxyribonucleic acid (DNA) molecules of simian virus 40 (SV40) have been cleaved by the R 1 restriction endonuclease from Escherichia coli. This enzyme introduces one double-strand break in SV40 DNA, at a specific site. The site of cleavage in the replicating molecules was used in this study to position the origin and the two branch points. Radioactively labeled molecules fractionated according to their extent of replication were evaluated after cleavage by sedimentation analysis and electron microscopy. The results demonstrate that the R 1 cleavage site is 33% of the genome length from the origin of replication and that both branch points are growing points. These data indicate that SV40 DNA replication is bidirectional and confirm other reports which have shown a unique origin of replication.

Bibliography

Fareed, G. C., Garon, C. F., & Salzman, N. P. (1972). Origin and Direction of Simian Virus 40 Deoxyribonucleic Acid Replication. Journal of Virology, 10(3), 484â491.

Authors 3

George C. Fareed (first)
Claude F. Garon (additional)
Norman P. Salzman (additional)

References 0 Referenced 209

None

Dates

Type	When
Created	5 years, 7 months ago (Jan. 5, 2020, 7:12 p.m.)
Deposited	3 years, 5 months ago (March 5, 2022, 11:01 a.m.)
Indexed	1 year ago (Aug. 5, 2024, 9:22 p.m.)
Issued	53 years ago (Sept. 1, 1972)
Published	53 years ago (Sept. 1, 1972)
Published Print	53 years ago (Sept. 1, 1972)

Funders 0

None

BibTeX

@article{Fareed_1972, title={Origin and Direction of Simian Virus 40 Deoxyribonucleic Acid Replication}, volume={10}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.10.3.484-491.1972}, DOI={10.1128/jvi.10.3.484-491.1972}, number={3}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Fareed, George C. and Garon, Claude F. and Salzman, Norman P.}, year={1972}, month=sep, pages={484–491} }

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