DOI: 10.1128/jvi.10.1.42-50.1972. Specific Fragments of φX174 Deoxyribonucleic Acid Produced by a Restriction Enzyme from <i>Haemophilus aegyptius</i> , Endonuclease Z

Specific Fragments of φX174 Deoxyribonucleic Acid Produced by a Restriction Enzyme from Haemophilus aegyptius , Endonuclease Z

10.1128/jvi.10.1.42-50.1972

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

A restriction-like enzyme has been purified from Haemophilus aegyptius. This nuclease, endonuclease Z, produces a rapid decrease in the viscosity of native calf thymus and H. influenzae deoxyribonucleic acids (DNA), but does not degrade homologous DNA. The specificity of endonuclease Z is different from that of the similar endonuclease isolated from H. influenzae (endonuclease R). The purified enzyme cleaves the double-stranded replicative form DNA of bacteriophage φX174 (φX174 RF DNA) into at least 11 specific limit fragments whose molecular sizes have been estimated by gel electrophoresis. The position of these fragments with respect to the genetic map of φX174 can be determined by using the genetic assay for small fragments of φX174 DNA.

Bibliography

Middleton, J. H., Edgell, M. H., & Hutchison, C. A. (1972). Specific Fragments of ÏX174 Deoxyribonucleic Acid Produced by a Restriction Enzyme from Haemophilus aegyptius , Endonuclease Z. Journal of Virology, 10(1), 42â50.

Authors 3

June H. Middleton (first)
Marshall H. Edgell (additional)
Clyde A. Hutchison (additional)

References 0 Referenced 163

None

Dates

Type	When
Created	5 years, 7 months ago (Jan. 5, 2020, 7:11 p.m.)
Deposited	3 years, 5 months ago (March 5, 2022, 11:01 a.m.)
Indexed	2 months, 3 weeks ago (June 3, 2025, 12:30 a.m.)
Issued	53 years, 1 month ago (July 1, 1972)
Published	53 years, 1 month ago (July 1, 1972)
Published Print	53 years, 1 month ago (July 1, 1972)

Funders 0

None

BibTeX

@article{Middleton_1972, title={Specific Fragments of φX174 Deoxyribonucleic Acid Produced by a Restriction Enzyme from Haemophilus aegyptius , Endonuclease Z}, volume={10}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.10.1.42-50.1972}, DOI={10.1128/jvi.10.1.42-50.1972}, number={1}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Middleton, June H. and Edgell, Marshall H. and Hutchison, Clyde A.}, year={1972}, month=jul, pages={42–50} }

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