DOI: 10.1128/jvi.1.2.362-367.1967. Adenovirus Type 12-Rat Embryo Transformation System

Adenovirus Type 12-Rat Embryo Transformation System

10.1128/jvi.1.2.362-367.1967

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

Adenovirus type 12 (Huie) inoculated into cultures of primary whole rat embryo produced foci of morphologically altered cells. The number and identification of these transformed areas was dependent upon the calcium concentration of the medium; more foci appeared in 0.1 m m than in 1.8 m m calcium. Cell lines derived from these inoculated cultures did not yield infectious virus, and also were similar to cell lines derived from adenovirus type 12-induced tumors with respect to morphology, presence of virus-specific tumor antigen, and oncogenicity. Dose-response curves revealed that transformation of rat embryo cells by adenovirus type 12 followed one-hit kinetics, and that approximately 7 × 10 5 infectious virus particles were required for one transformation event. Our results indicate that the transformation system described for adenovirus type 12 is reproducible, and that previous difficulties experienced in developing such a system may well be explained by the higher calcium concentration of the tissue culture media used.

Bibliography

Freeman, A. E., Black, P. H., Wolford, R., & Huebner, R. J. (1967). Adenovirus Type 12-Rat Embryo Transformation System. Journal of Virology, 1(2), 362â367.

Authors 4

Aaron E. Freeman (first)
Paul H. Black (additional)
Ronald Wolford (additional)
Robert J. Huebner (additional)

References 0 Referenced 79

None

Dates

Type	When
Created	5 years, 8 months ago (Jan. 5, 2020, 7:29 p.m.)
Deposited	3 years, 6 months ago (March 5, 2022, 10:56 a.m.)
Indexed	1 year, 1 month ago (July 22, 2024, 9:54 a.m.)
Issued	58 years, 5 months ago (April 1, 1967)
Published	58 years, 5 months ago (April 1, 1967)
Published Print	58 years, 5 months ago (April 1, 1967)

Funders 0

None

BibTeX

@article{Freeman_1967, title={Adenovirus Type 12-Rat Embryo Transformation System}, volume={1}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.1.2.362-367.1967}, DOI={10.1128/jvi.1.2.362-367.1967}, number={2}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Freeman, Aaron E. and Black, Paul H. and Wolford, Ronald and Huebner, Robert J.}, year={1967}, month=apr, pages={362–367} }

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