DOI: 10.1128/jvi.07232-11. Structural Insight into the Unique Properties of Adeno-Associated Virus Serotype 9

Structural Insight into the Unique Properties of Adeno-Associated Virus Serotype 9

10.1128/jvi.07232-11

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT Adeno-associated virus serotype 9 (AAV9) has enhanced capsid-associated tropism for cardiac muscle and the ability to cross the blood-brain barrier compared to other AAV serotypes. To help identify the structural features facilitating these properties, we have used cryo-electron microscopy (cryo-EM) and three-dimensional image reconstruction (cryo-reconstruction) and X-ray crystallography to determine the structure of the AAV9 capsid at 9.7- and 2.8-Å resolutions, respectively. The AAV9 capsid exhibits the surface topology conserved in all AAVs: depressions at each icosahedral two-fold symmetry axis and surrounding each five-fold axis, three separate protrusions surrounding each three-fold axis, and a channel at each five-fold axis. The AAV9 viral protein (VP) has a conserved core structure, consisting of an eight-stranded, β-barrel motif and the αA helix, which are present in all parvovirus structures. The AAV9 VP differs in nine variable surface regions (VR-I to -IX) compared to AAV4, but at only three (VR-I, VR-II, and VR-IV) compared to AAV2 and AAV8. VR-I differences modify the raised region of the capsid surface between the two-fold and five-fold depressions. The VR-IV difference produces smaller three-fold protrusions in AAV9 that are less “pointed” than AAV2 and AAV8. Significantly, residues in the AAV9 VRs have been identified as important determinants of cellular tropism and transduction and dictate its antigenic diversity from AAV2. Hence, the AAV9 VRs likely confer the unique infection phenotypes of this serotype.

Bibliography

DiMattia, M. A., Nam, H.-J., Van Vliet, K., Mitchell, M., Bennett, A., Gurda, B. L., McKenna, R., Olson, N. H., Sinkovits, R. S., Potter, M., Byrne, B. J., Aslanidi, G., Zolotukhin, S., Muzyczka, N., Baker, T. S., & Agbandje-McKenna, M. (2012). Structural Insight into the Unique Properties of Adeno-Associated Virus Serotype 9. Journal of Virology, 86(12), 6947â6958.

Authors 16

Michael A. DiMattia (first)
Hyun-Joo Nam (additional)
Kim Van Vliet (additional)
Matthew Mitchell (additional)
Antonette Bennett (additional)
Brittney L. Gurda (additional)
Robert McKenna (additional)
Norman H. Olson (additional)
Robert S. Sinkovits (additional)
Mark Potter (additional)
Barry J. Byrne (additional)
George Aslanidi (additional)
Sergei Zolotukhin (additional)
Nicholas Muzyczka (additional)
Timothy S. Baker (additional)
Mavis Agbandje-McKenna (additional)

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Dates

Type	When
Created	13 years, 4 months ago (April 12, 2012, 1:16 a.m.)
Deposited	3 years, 5 months ago (March 4, 2022, 4:42 p.m.)
Indexed	3 days ago (Aug. 31, 2025, 6:09 a.m.)
Issued	13 years, 2 months ago (June 15, 2012)
Published	13 years, 2 months ago (June 15, 2012)
Published Print	13 years, 2 months ago (June 15, 2012)

Funders 0

None

BibTeX

@article{DiMattia_2012, title={Structural Insight into the Unique Properties of Adeno-Associated Virus Serotype 9}, volume={86}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.07232-11}, DOI={10.1128/jvi.07232-11}, number={12}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={DiMattia, Michael A. and Nam, Hyun-Joo and Van Vliet, Kim and Mitchell, Matthew and Bennett, Antonette and Gurda, Brittney L. and McKenna, Robert and Olson, Norman H. and Sinkovits, Robert S. and Potter, Mark and Byrne, Barry J. and Aslanidi, George and Zolotukhin, Sergei and Muzyczka, Nicholas and Baker, Timothy S. and Agbandje-McKenna, Mavis}, year={2012}, month=jun, pages={6947–6958} }

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