DOI: 10.1128/jvi.05564-11. <i>In Vitro</i> Assembly of Virus-Like Particles of a Gammaretrovirus, the Murine Leukemia Virus XMRV

In Vitro Assembly of Virus-Like Particles of a Gammaretrovirus, the Murine Leukemia Virus XMRV

10.1128/jvi.05564-11

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT Immature retroviral particles are assembled by self-association of the structural polyprotein precursor Gag. During maturation the Gag polyprotein is proteolytically cleaved, yielding mature structural proteins, matrix (MA), capsid (CA), and nucleocapsid (NC), that reassemble into a mature viral particle. Proteolytic cleavage causes the N terminus of CA to fold back to form a β-hairpin, anchored by an internal salt bridge between the N-terminal proline and the inner aspartate. Using an in vitro assembly system of capsid-nucleocapsid protein (CANC), we studied the formation of virus-like particles (VLP) of a gammaretrovirus, the xenotropic murine leukemia virus (MLV)-related virus (XMRV). We show here that, unlike other retroviruses, XMRV CA and CANC do not assemble tubular particles characteristic of mature assembly. The prevention of β-hairpin formation by the deletion of either the N-terminal proline or 10 initial amino acids enabled the assembly of ΔProCANC or Δ10CANC into immature-like spherical particles. Detailed three-dimensional (3D) structural analysis of these particles revealed that below a disordered N-terminal CA layer, the C terminus of CA assembles a typical immature lattice, which is linked by rod-like densities with the RNP.

Bibliography

HadravovÃ¡, R., de Marco, A., Ulbrich, P., Å tokrovÃ¡, J., DoleÅ¾al, M., PichovÃ¡, I., Ruml, T., Briggs, J. A. G., & RumlovÃ¡, M. (2012). In Vitro Assembly of Virus-Like Particles of a Gammaretrovirus, the Murine Leukemia Virus XMRV. Journal of Virology, 86(3), 1297â1306.

Authors 9

Romana Hadravová (first)
Alex de Marco (additional)
Pavel Ulbrich (additional)
Jitka Štokrová (additional)
Michal Doležal (additional)
Iva Pichová (additional)
Tomáš Ruml (additional)
John A. G. Briggs (additional)
Michaela Rumlová (additional)

References 68 Referenced 20

Dates

Type	When
Created	13 years, 9 months ago (Nov. 17, 2011, 7:27 a.m.)
Deposited	3 years, 5 months ago (March 5, 2022, 10:21 a.m.)
Indexed	1 year, 1 month ago (July 7, 2024, 6:31 a.m.)
Issued	13 years, 7 months ago (Feb. 1, 2012)
Published	13 years, 7 months ago (Feb. 1, 2012)
Published Print	13 years, 7 months ago (Feb. 1, 2012)

Funders 0

None

BibTeX

@article{Hadravov__2012, title={In Vitro Assembly of Virus-Like Particles of a Gammaretrovirus, the Murine Leukemia Virus XMRV}, volume={86}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.05564-11}, DOI={10.1128/jvi.05564-11}, number={3}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Hadravová, Romana and de Marco, Alex and Ulbrich, Pavel and Štokrová, Jitka and Doležal, Michal and Pichová, Iva and Ruml, Tomáš and Briggs, John A. G. and Rumlová, Michaela}, year={2012}, month=feb, pages={1297–1306} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        7,
        7
      ]
    ],
    "date-time": "2024-07-07T10:31:57Z",
    "timestamp": 1720348317825
  },
  "reference-count": 68,
  "publisher": "American Society for Microbiology",
  "issue": "3",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2012,
            2,
            1
          ]
        ],
        "date-time": "2012-02-01T00:00:00Z",
        "timestamp": 1328054400000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://journals.asm.org/non-commercial-tdm-license"
    }
  ],
  "content-domain": {
    "domain": [
      "journals.asm.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2012,
        2
      ]
    ]
  },
  "abstract": "<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            Immature retroviral particles are assembled by self-association of the structural polyprotein precursor Gag. During maturation the Gag polyprotein is proteolytically cleaved, yielding mature structural proteins, matrix (MA), capsid (CA), and nucleocapsid (NC), that reassemble into a mature viral particle. Proteolytic cleavage causes the N terminus of CA to fold back to form a \u03b2-hairpin, anchored by an internal salt bridge between the N-terminal proline and the inner aspartate. Using an\n            <jats:italic>in vitro</jats:italic>\n            assembly system of capsid-nucleocapsid protein (CANC), we studied the formation of virus-like particles (VLP) of a gammaretrovirus, the xenotropic murine leukemia virus (MLV)-related virus (XMRV). We show here that, unlike other retroviruses, XMRV CA and CANC do not assemble tubular particles characteristic of mature assembly. The prevention of \u03b2-hairpin formation by the deletion of either the N-terminal proline or 10 initial amino acids enabled the assembly of \u0394ProCANC or \u039410CANC into immature-like spherical particles. Detailed three-dimensional (3D) structural analysis of these particles revealed that below a disordered N-terminal CA layer, the C terminus of CA assembles a typical immature lattice, which is linked by rod-like densities with the RNP.\n          </jats:p>",
  "DOI": "10.1128/jvi.05564-11",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2011,
        11,
        17
      ]
    ],
    "date-time": "2011-11-17T12:27:45Z",
    "timestamp": 1321532865000
  },
  "page": "1297-1306",
  "update-policy": "http://dx.doi.org/10.1128/asmj-crossmark-policy-page",
  "source": "Crossref",
  "is-referenced-by-count": 20,
  "title": "<i>In Vitro</i>\n            Assembly of Virus-Like Particles of a Gammaretrovirus, the Murine Leukemia Virus XMRV",
  "prefix": "10.1128",
  "volume": "86",
  "author": [
    {
      "given": "Romana",
      "family": "Hadravov\u00e1",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i., IOCB and Gilead Research Center, Prague, Czech Republic"
        }
      ]
    },
    {
      "given": "Alex",
      "family": "de Marco",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Structural and Computational Unit, European Molecular Biology Laboratory, Heidelberg, Germany"
        }
      ]
    },
    {
      "given": "Pavel",
      "family": "Ulbrich",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Czech Republic"
        }
      ]
    },
    {
      "given": "Jitka",
      "family": "\u0160tokrov\u00e1",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i., IOCB and Gilead Research Center, Prague, Czech Republic"
        }
      ]
    },
    {
      "given": "Michal",
      "family": "Dole\u017eal",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i., IOCB and Gilead Research Center, Prague, Czech Republic"
        },
        {
          "name": "Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Czech Republic"
        }
      ]
    },
    {
      "given": "Iva",
      "family": "Pichov\u00e1",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i., IOCB and Gilead Research Center, Prague, Czech Republic"
        }
      ]
    },
    {
      "given": "Tom\u00e1\u0161",
      "family": "Ruml",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Czech Republic"
        }
      ]
    },
    {
      "given": "John A. G.",
      "family": "Briggs",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Structural and Computational Unit, European Molecular Biology Laboratory, Heidelberg, Germany"
        }
      ]
    },
    {
      "given": "Michaela",
      "family": "Rumlov\u00e1",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i., IOCB and Gilead Research Center, Prague, Czech Republic"
        }
      ]
    }
  ],
  "member": "235",
  "reference": [
    {
      "key": "e_1_3_2_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.72.3.2072-2078.1998"
    },
    {
      "key": "e_1_3_2_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.74.12.5395-5402.2000"
    },
    {
      "key": "e_1_3_2_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.02022-09"
    },
    {
      "key": "e_1_3_2_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.72.11.9313-9317.1998"
    },
    {
      "key": "e_1_3_2_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.jmb.2005.10.025"
    },
    {
      "key": "e_1_3_2_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0903535106"
    },
    {
      "key": "e_1_3_2_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nsmb785"
    },
    {
      "key": "e_1_3_2_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.69.10.6487-6497.1995"
    },
    {
      "key": "e_1_3_2_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.77.12.7058-7066.2003"
    },
    {
      "key": "e_1_3_2_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jmbi.2000.4395"
    },
    {
      "key": "e_1_3_2_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.05889-11"
    },
    {
      "key": "e_1_3_2_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.01423-10"
    },
    {
      "key": "e_1_3_2_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1371/journal.ppat.1001215"
    },
    {
      "key": "e_1_3_2_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.66.8.4874-4883.1992"
    },
    {
      "key": "e_1_3_2_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1371/journal.pone.0017592"
    },
    {
      "key": "e_1_3_2_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.jcv.2008.04.016"
    },
    {
      "key": "e_1_3_2_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1186/1742-4690-8-20"
    },
    {
      "key": "e_1_3_2_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.283.5398.80"
    },
    {
      "key": "e_1_3_2_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.sbi.2008.02.001"
    },
    {
      "key": "e_1_3_2_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.273.5272.231"
    },
    {
      "key": "e_1_3_2_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.72.6.4798-4810.1998"
    },
    {
      "key": "e_1_3_2_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1997.t01-1-00592.x"
    },
    {
      "key": "e_1_3_2_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/19.1.103"
    },
    {
      "key": "e_1_3_2_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.1100118108"
    },
    {
      "key": "e_1_3_2_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1186/1742-4690-6-92"
    },
    {
      "key": "e_1_3_2_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1186/1743-422X-7-224"
    },
    {
      "key": "e_1_3_2_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.75.9.4407-4412.2001"
    },
    {
      "key": "e_1_3_2_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.00213-08"
    },
    {
      "key": "e_1_3_2_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jmbi.1999.2986"
    },
    {
      "key": "e_1_3_2_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0969-2126(00)00148-9"
    },
    {
      "key": "e_1_3_2_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.69.2.1093-1098.1995"
    },
    {
      "key": "e_1_3_2_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1204963"
    },
    {
      "key": "e_1_3_2_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.69.6.3407-3419.1995"
    },
    {
      "key": "e_1_3_2_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jsbi.1996.0013"
    },
    {
      "key": "e_1_3_2_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.virol.2006.10.015"
    },
    {
      "key": "e_1_3_2_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35030177"
    },
    {
      "key": "e_1_3_2_38_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.77.3.1772-1783.2003"
    },
    {
      "key": "e_1_3_2_39_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1179052"
    },
    {
      "key": "e_1_3_2_40_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jsbi.1999.4174"
    },
    {
      "key": "e_1_3_2_41_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.76.11.5452-5462.2002"
    },
    {
      "key": "e_1_3_2_42_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.78.1.52-60.2004"
    },
    {
      "key": "e_1_3_2_43_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.jmb.2009.06.029"
    },
    {
      "key": "e_1_3_2_44_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.jmb.2007.12.043"
    },
    {
      "key": "e_1_3_2_45_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nature02915"
    },
    {
      "key": "e_1_3_2_46_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1205292"
    },
    {
      "key": "e_1_3_2_47_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/emboj.2008.71"
    },
    {
      "key": "e_1_3_2_48_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nature09640"
    },
    {
      "key": "e_1_3_2_49_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0042-6822(02)00074-0"
    },
    {
      "key": "e_1_3_2_50_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.tim.2011.08.005"
    },
    {
      "key": "e_1_3_2_51_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.74.18.8452-8459.2000"
    },
    {
      "key": "e_1_3_2_52_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1186/1742-4690-8-12"
    },
    {
      "key": "e_1_3_2_53_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/ana.22389"
    },
    {
      "key": "e_1_3_2_54_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/pros.20680"
    },
    {
      "key": "e_1_3_2_55_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.00693-11"
    },
    {
      "key": "e_1_3_2_56_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.virusres.2010.10.001"
    },
    {
      "key": "e_1_3_2_57_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1186/1742-4690-7-57"
    },
    {
      "key": "e_1_3_2_58_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.02694-05"
    },
    {
      "key": "e_1_3_2_59_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1371/journal.ppat.0020025"
    },
    {
      "key": "e_1_3_2_60_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jsbi.1996.0004"
    },
    {
      "key": "e_1_3_2_61_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/pros.21255"
    },
    {
      "key": "e_1_3_2_62_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/17.6.1555"
    },
    {
      "key": "e_1_3_2_63_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.77.9.5439-5450.2003"
    },
    {
      "key": "e_1_3_2_64_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.77.5.3339-3344.2003"
    },
    {
      "key": "e_1_3_2_65_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.virol.2008.07.021"
    },
    {
      "key": "e_1_3_2_66_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.75.2.759-771.2001"
    },
    {
      "key": "e_1_3_2_67_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/sj.emboj.7601664"
    },
    {
      "key": "e_1_3_2_68_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.95.13.7299"
    },
    {
      "key": "e_1_3_2_69_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.75.6.2753-2764.2001"
    }
  ],
  "container-title": "Journal of Virology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/JVI.05564-11",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/JVI.05564-11",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        3,
        5
      ]
    ],
    "date-time": "2022-03-05T15:21:49Z",
    "timestamp": 1646493709000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.asm.org/doi/10.1128/JVI.05564-11"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2012,
        2
      ]
    ]
  },
  "references-count": 68,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          2012,
          2
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1128/JVI.05564-11"
  ],
  "URL": "http://dx.doi.org/10.1128/JVI.05564-11",
  "relation": {},
  "ISSN": [
    "0022-538X",
    "1098-5514"
  ],
  "subject": [],
  "container-title-short": "J Virol",
  "published": {
    "date-parts": [
      [
        2012,
        2
      ]
    ]
  },
  "assertion": [
    {
      "value": "2011-06-30",
      "order": 0,
      "name": "received",
      "label": "Received",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2011-11-06",
      "order": 1,
      "name": "accepted",
      "label": "Accepted",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2012-01-12",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}