DOI: 10.1128/jvi.02090-08. Translocation of Incoming Pseudorabies Virus Capsids to the Cell Nucleus Is Delayed in the Absence of Tegument Protein pUL37

Translocation of Incoming Pseudorabies Virus Capsids to the Cell Nucleus Is Delayed in the Absence of Tegument Protein pUL37

10.1128/jvi.02090-08

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT After fusion of the envelope of herpesvirus particles with the host cell plasma membrane, incoming nucleocapsids are transported to nuclear pores. Inner tegument proteins pUL36, pUL37, and pUS3 remain attached to the nucleocapsid after entry and therefore might mediate interactions between the nucleocapsid and cellular microtubule-associated motor proteins during transport. To assay for the role of pUL37 in this process, we constructed a pUL37-deleted pseudorabies virus mutant, PrV-ΔUL37/UL35GFP, which expresses a fusion protein of green fluorescent protein (GFP) and the nonessential small capsid protein pUL35, resulting in the formation of fluorescently labeled capsids. Confocal laser-scanning microscopy of rabbit kidney cells infected with PrV-ΔUL37/UL35GFP revealed that, whereas penetration was not affected in the absence of pUL37, nuclear translocation of incoming particles was delayed by approximately 1 h compared to PrV-UL35GFP, but not abolished. In contrast, phenotypically complemented pUL37-containing virions of PrV-ΔUL37/UL35GFP exhibited wild type-like entry kinetics. Thus, the presence of pUL37 is required for rapid nuclear translocation of incoming nucleocapsids.

Bibliography

Krautwald, M., Fuchs, W., Klupp, B. G., & Mettenleiter, T. C. (2009). Translocation of Incoming Pseudorabies Virus Capsids to the Cell Nucleus Is Delayed in the Absence of Tegument Protein pUL37. Journal of Virology, 83(7), 3389â3396.

Authors 4

Mirjam Krautwald (first)
Walter Fuchs (additional)
Barbara G. Klupp (additional)
Thomas C. Mettenleiter (additional)

References 55 Referenced 46

10.1128/JVI.00026-06
10.1128/jvi.46.2.371-377.1983
10.1128/JVI.76.3.1043-1050.2002
10.1006/viro.1998.9230
10.1128/JVI.72.9.7563-7568.1998
10.1128/JVI.75.21.10259-10271.2001
10.1016/j.bbrc.2004.05.069
10.1128/JVI.76.7.3282-3291.2002
10.1016/j.tim.2005.05.010
10.1128/JVI.02528-05
Döhner, K., and B. Sodeik. 2005. The role of cytoskeleton during viral infection. Curr. Top. Microbiol. Immunol.2005:67-108. / Curr. Top. Microbiol. Immunol. (2005)
10.1091/mbc.01-07-0348
10.1074/jbc.M311671200
10.1128/JVI.00069-07
10.1128/JVI.00057-06
10.1128/JVI.78.21.11879-11889.2004
10.1016/j.addr.2003.07.006
10.1159/000150509
10.1128/JVI.79.5.3200-3205.2005
10.1128/jvi.71.3.2072-2082.1997
Harmon, M. E., and W. Gibson. 1996. High molecular weight virion protein of human cytomegalovirus forms complex with product of adjacent open reading frame, abstr. W35-4, p.144. Proc. Am. Soc. Virol., London, Ontario, Canada. / Proc. Am. Soc. Virol. (1996)
10.1016/0042-6822(59)90068-6
10.1128/JVI.02589-05
10.1128/JVI.76.6.3065-3071.2002
10.1128/JVI.75.19.8927-8936.2001
10.1099/vir.0.80633-0
10.1099/vir.0.83652-0
10.1099/0022-1317-67-9-2023
10.1128/jvi.63.11.4834-4839.1989
10.1089/10430340050016238
10.1073/pnas.0500803102
10.1128/JVI.80.1.201-209.2006
10.1128/JVI.76.19.9962-9971.2002
10.1016/S0014-5793(03)00516-7
10.1083/jcb.200203150
10.1016/0042-6822(89)90635-1
10.1016/j.vetmic.2005.11.040
10.1146/annurev.biophys.30.1.397
Reference deleted.
10.1128/JVI.77.15.8541-8547.2003
10.1016/S0955-0674(00)00180-0
10.1006/viro.1999.9673
10.1111/j.1462-5822.2005.00679.x
10.1146/annurev.cellbio.20.012103.094623
10.1146/annurev.cellbio.18.012502.105920
10.1073/pnas.061029798
10.1073/pnas.0404686101
10.1083/jcb.136.5.1007
10.1016/j.virol.2005.09.016
10.1128/JVI.77.19.10270-10279.2003
10.1034/j.1600-0854.2001.020701.x
10.1016/0306-4522(95)00497-1
10.1002/neu.10314
10.1111/j.1600-0854.2005.00379.x
10.1128/JVI.74.3.1355-1363.2000

Dates

Type	When
Created	16 years, 7 months ago (Jan. 14, 2009, 10:35 p.m.)
Deposited	3 years, 6 months ago (March 5, 2022, 7:53 a.m.)
Indexed	2 months, 1 week ago (June 27, 2025, 6:40 p.m.)
Issued	16 years, 5 months ago (April 1, 2009)
Published	16 years, 5 months ago (April 1, 2009)
Published Print	16 years, 5 months ago (April 1, 2009)

Funders 0

None

BibTeX

@article{Krautwald_2009, title={Translocation of Incoming Pseudorabies Virus Capsids to the Cell Nucleus Is Delayed in the Absence of Tegument Protein pUL37}, volume={83}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.02090-08}, DOI={10.1128/jvi.02090-08}, number={7}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Krautwald, Mirjam and Fuchs, Walter and Klupp, Barbara G. and Mettenleiter, Thomas C.}, year={2009}, month=apr, pages={3389–3396} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        6,
        27
      ]
    ],
    "date-time": "2025-06-27T22:40:57Z",
    "timestamp": 1751064057751
  },
  "reference-count": 55,
  "publisher": "American Society for Microbiology",
  "issue": "7",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2009,
            4,
            1
          ]
        ],
        "date-time": "2009-04-01T00:00:00Z",
        "timestamp": 1238544000000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://journals.asm.org/non-commercial-tdm-license"
    }
  ],
  "content-domain": {
    "domain": [
      "journals.asm.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2009,
        4
      ]
    ]
  },
  "abstract": "<jats:title>ABSTRACT</jats:title>\n          <jats:p>After fusion of the envelope of herpesvirus particles with the host cell plasma membrane, incoming nucleocapsids are transported to nuclear pores. Inner tegument proteins pUL36, pUL37, and pUS3 remain attached to the nucleocapsid after entry and therefore might mediate interactions between the nucleocapsid and cellular microtubule-associated motor proteins during transport. To assay for the role of pUL37 in this process, we constructed a pUL37-deleted pseudorabies virus mutant, PrV-\u0394UL37/UL35GFP, which expresses a fusion protein of green fluorescent protein (GFP) and the nonessential small capsid protein pUL35, resulting in the formation of fluorescently labeled capsids. Confocal laser-scanning microscopy of rabbit kidney cells infected with PrV-\u0394UL37/UL35GFP revealed that, whereas penetration was not affected in the absence of pUL37, nuclear translocation of incoming particles was delayed by approximately 1 h compared to PrV-UL35GFP, but not abolished. In contrast, phenotypically complemented pUL37-containing virions of PrV-\u0394UL37/UL35GFP exhibited wild type-like entry kinetics. Thus, the presence of pUL37 is required for rapid nuclear translocation of incoming nucleocapsids.</jats:p>",
  "DOI": "10.1128/jvi.02090-08",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2009,
        1,
        15
      ]
    ],
    "date-time": "2009-01-15T03:35:35Z",
    "timestamp": 1231990535000
  },
  "page": "3389-3396",
  "update-policy": "http://dx.doi.org/10.1128/asmj-crossmark-policy-page",
  "source": "Crossref",
  "is-referenced-by-count": 46,
  "title": "Translocation of Incoming Pseudorabies Virus Capsids to the Cell Nucleus Is Delayed in the Absence of Tegument Protein pUL37",
  "prefix": "10.1128",
  "volume": "83",
  "author": [
    {
      "given": "Mirjam",
      "family": "Krautwald",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Institute of Molecular Biology, Friedrich-Loeffler-Institut, Su\u0308dufer 10, 17493 Greifswald-Insel Riems, Germany"
        }
      ]
    },
    {
      "given": "Walter",
      "family": "Fuchs",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Institute of Molecular Biology, Friedrich-Loeffler-Institut, Su\u0308dufer 10, 17493 Greifswald-Insel Riems, Germany"
        }
      ]
    },
    {
      "given": "Barbara G.",
      "family": "Klupp",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Institute of Molecular Biology, Friedrich-Loeffler-Institut, Su\u0308dufer 10, 17493 Greifswald-Insel Riems, Germany"
        }
      ]
    },
    {
      "given": "Thomas C.",
      "family": "Mettenleiter",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Institute of Molecular Biology, Friedrich-Loeffler-Institut, Su\u0308dufer 10, 17493 Greifswald-Insel Riems, Germany"
        }
      ]
    }
  ],
  "member": "235",
  "reference": [
    {
      "key": "e_1_3_2_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.00026-06"
    },
    {
      "key": "e_1_3_2_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.46.2.371-377.1983"
    },
    {
      "key": "e_1_3_2_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.76.3.1043-1050.2002"
    },
    {
      "key": "e_1_3_2_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/viro.1998.9230"
    },
    {
      "key": "e_1_3_2_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.72.9.7563-7568.1998"
    },
    {
      "key": "e_1_3_2_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.75.21.10259-10271.2001"
    },
    {
      "key": "e_1_3_2_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.bbrc.2004.05.069"
    },
    {
      "key": "e_1_3_2_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.76.7.3282-3291.2002"
    },
    {
      "key": "e_1_3_2_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.tim.2005.05.010"
    },
    {
      "key": "e_1_3_2_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.02528-05"
    },
    {
      "key": "e_1_3_2_12_2",
      "first-page": "67",
      "volume": "2005",
      "year": "2005",
      "unstructured": "D\u00f6hner, K., and B. Sodeik. 2005. The role of cytoskeleton during viral infection. Curr. Top. Microbiol. Immunol.2005:67-108.",
      "journal-title": "Curr. Top. Microbiol. Immunol."
    },
    {
      "key": "e_1_3_2_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.01-07-0348"
    },
    {
      "key": "e_1_3_2_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M311671200"
    },
    {
      "key": "e_1_3_2_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.00069-07"
    },
    {
      "key": "e_1_3_2_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.00057-06"
    },
    {
      "key": "e_1_3_2_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.78.21.11879-11889.2004"
    },
    {
      "key": "e_1_3_2_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.addr.2003.07.006"
    },
    {
      "key": "e_1_3_2_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1159/000150509"
    },
    {
      "key": "e_1_3_2_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.79.5.3200-3205.2005"
    },
    {
      "key": "e_1_3_2_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.71.3.2072-2082.1997"
    },
    {
      "key": "e_1_3_2_22_2",
      "first-page": "144",
      "year": "1996",
      "unstructured": "Harmon, M. E., and W. Gibson. 1996. High molecular weight virion protein of human cytomegalovirus forms complex with product of adjacent open reading frame, abstr. W35-4, p.144. Proc. Am. Soc. Virol., London, Ontario, Canada.",
      "journal-title": "Proc. Am. Soc. Virol."
    },
    {
      "key": "e_1_3_2_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0042-6822(59)90068-6"
    },
    {
      "key": "e_1_3_2_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.02589-05"
    },
    {
      "key": "e_1_3_2_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.76.6.3065-3071.2002"
    },
    {
      "key": "e_1_3_2_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.75.19.8927-8936.2001"
    },
    {
      "key": "e_1_3_2_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1099/vir.0.80633-0"
    },
    {
      "key": "e_1_3_2_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1099/vir.0.83652-0"
    },
    {
      "key": "e_1_3_2_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1099/0022-1317-67-9-2023"
    },
    {
      "key": "e_1_3_2_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.63.11.4834-4839.1989"
    },
    {
      "key": "e_1_3_2_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1089/10430340050016238"
    },
    {
      "key": "e_1_3_2_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0500803102"
    },
    {
      "key": "e_1_3_2_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.80.1.201-209.2006"
    },
    {
      "key": "e_1_3_2_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.76.19.9962-9971.2002"
    },
    {
      "key": "e_1_3_2_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0014-5793(03)00516-7"
    },
    {
      "key": "e_1_3_2_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.200203150"
    },
    {
      "key": "e_1_3_2_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0042-6822(89)90635-1"
    },
    {
      "key": "e_1_3_2_38_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.vetmic.2005.11.040"
    },
    {
      "key": "e_1_3_2_39_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.biophys.30.1.397"
    },
    {
      "key": "e_1_3_2_40_2",
      "unstructured": "Reference deleted."
    },
    {
      "key": "e_1_3_2_41_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.77.15.8541-8547.2003"
    },
    {
      "key": "e_1_3_2_42_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0955-0674(00)00180-0"
    },
    {
      "key": "e_1_3_2_43_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/viro.1999.9673"
    },
    {
      "key": "e_1_3_2_44_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1462-5822.2005.00679.x"
    },
    {
      "key": "e_1_3_2_45_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.cellbio.20.012103.094623"
    },
    {
      "key": "e_1_3_2_46_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.cellbio.18.012502.105920"
    },
    {
      "key": "e_1_3_2_47_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.061029798"
    },
    {
      "key": "e_1_3_2_48_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0404686101"
    },
    {
      "key": "e_1_3_2_49_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.136.5.1007"
    },
    {
      "key": "e_1_3_2_50_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.virol.2005.09.016"
    },
    {
      "key": "e_1_3_2_51_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.77.19.10270-10279.2003"
    },
    {
      "key": "e_1_3_2_52_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1034/j.1600-0854.2001.020701.x"
    },
    {
      "key": "e_1_3_2_53_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0306-4522(95)00497-1"
    },
    {
      "key": "e_1_3_2_54_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/neu.10314"
    },
    {
      "key": "e_1_3_2_55_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1600-0854.2005.00379.x"
    },
    {
      "key": "e_1_3_2_56_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.74.3.1355-1363.2000"
    }
  ],
  "container-title": "Journal of Virology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/JVI.02090-08",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/JVI.02090-08",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        3,
        5
      ]
    ],
    "date-time": "2022-03-05T12:53:20Z",
    "timestamp": 1646484800000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.asm.org/doi/10.1128/JVI.02090-08"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2009,
        4
      ]
    ]
  },
  "references-count": 55,
  "journal-issue": {
    "issue": "7",
    "published-print": {
      "date-parts": [
        [
          2009,
          4
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1128/JVI.02090-08"
  ],
  "URL": "http://dx.doi.org/10.1128/JVI.02090-08",
  "relation": {},
  "ISSN": [
    "0022-538X",
    "1098-5514"
  ],
  "subject": [],
  "container-title-short": "J Virol",
  "published": {
    "date-parts": [
      [
        2009,
        4
      ]
    ]
  },
  "assertion": [
    {
      "value": "2008-10-04",
      "order": 0,
      "name": "received",
      "label": "Received",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2009-01-06",
      "order": 1,
      "name": "accepted",
      "label": "Accepted",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2009-04-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}