DOI: 10.1128/jvi.01880-06. White Spot Syndrome Virus Annexes a Shrimp STAT To Enhance Expression of the Immediate-Early Gene <i>ie1</i>

White Spot Syndrome Virus Annexes a Shrimp STAT To Enhance Expression of the Immediate-Early Gene ie1

10.1128/jvi.01880-06

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT Although the Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling pathway is part of the antiviral response in arthropods such as Drosophila , here we show that white spot syndrome virus (WSSV) uses a shrimp STAT as a transcription factor to enhance viral gene expression in host cells. In a series of deletion and mutation assays using the WSSV immediate-early gene ie1 promoter, which is active in shrimp cells and also in insect Sf9 cells, an element containing a STAT binding motif was shown to be important for the overall level of WSSV ie1 promoter activity. In the Sf9 insect cell line, a specific protein-DNA complex was detected by using electrophoresis mobility shift assays (EMSA) with the 32 P-labeled STAT binding motif of the WSSV ie1 promoter as the probe. When recombinant Penaeus monodon STAT (rPmSTAT) was overexpressed in Sf9 cells, EMSA with specific antibodies confirmed that the STAT was responsible for the formation of the specific protein-DNA complex. Another EMSA showed that in WSSV-infected P. monodon , levels of activated PmSTAT were higher than in WSSV-free P. monodon . A transactivation assay of the WSSV ie1 promoter demonstrated that increasing the level of rPmSTAT led to dose-dependent increases in ie1 promoter activity. These results show that STAT directly transactivates WSSV ie1 gene expression and contributes to its high promoter activity. We conclude that WSSV successfully annexes a putative shrimp defense mechanism, which it uses to enhance the expression of viral immediate-early genes.

Bibliography

Liu, W.-J., Chang, Y.-S., Wang, A. H.-J., Kou, G.-H., & Lo, C.-F. (2007). White Spot Syndrome Virus Annexes a Shrimp STAT To Enhance Expression of the Immediate-Early Gene ie1. Journal of Virology, 81(3), 1461â1471.

Authors 5

Wang-Jing Liu (first)
Yun-Shiang Chang (additional)
Andrew H.-J. Wang (additional)
Guang-Hsiung Kou (additional)
Chu-Fang Lo (additional)

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Dates

Type	When
Created	18 years, 7 months ago (Jan. 16, 2007, 9:53 p.m.)
Deposited	3 years, 5 months ago (March 5, 2022, 6:31 a.m.)
Indexed	3 weeks, 6 days ago (Aug. 2, 2025, 1 a.m.)
Issued	18 years, 6 months ago (Feb. 1, 2007)
Published	18 years, 6 months ago (Feb. 1, 2007)
Published Print	18 years, 6 months ago (Feb. 1, 2007)

Funders 0

None

BibTeX

@article{Liu_2007, title={White Spot Syndrome Virus Annexes a Shrimp STAT To Enhance Expression of the Immediate-Early Gene ie1}, volume={81}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.01880-06}, DOI={10.1128/jvi.01880-06}, number={3}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Liu, Wang-Jing and Chang, Yun-Shiang and Wang, Andrew H.-J. and Kou, Guang-Hsiung and Lo, Chu-Fang}, year={2007}, month=feb, pages={1461–1471} }

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