DOI: 10.1128/jvi.01801-06. Efficient Incorporation of Tegument Proteins pUL46, pUL49, and pUS3 into Pseudorabies Virus Particles Depends on the Presence of pUL21

Efficient Incorporation of Tegument Proteins pUL46, pUL49, and pUS3 into Pseudorabies Virus Particles Depends on the Presence of pUL21

10.1128/jvi.01801-06

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT The mature virion of the alphaherpesvirus pseudorabies virus (PrV) contains a minimum of 31 structural proteins which are recruited into the virus particle by a network of protein-protein interactions which is only incompletely understood. We show here that deletion of the tegument protein pUL21 resulted in a drastic decrease in the incorporation of the pUL46, pUL49, and pUS3 tegument components into mature virions. Moreover, the attenuated PrV strain Bartha (PrV-Ba), which, among other defects, carries mutations in pUL21, also fails to package pUL46, pUL49, and pUS3 efficiently. By the reconstitution of wild-type pUL21 expression to PrV-Ba and the transfer of mutated PrV-Ba pUL21 into wild-type PrV, we demonstrate that this phenotype is due to the mutated pUL21.

Bibliography

Michael, K., Klupp, B. G., Karger, A., & Mettenleiter, T. C. (2007). Efficient Incorporation of Tegument Proteins pUL46, pUL49, and pUS3 into Pseudorabies Virus Particles Depends on the Presence of pUL21. Journal of Virology, 81(2), 1048â1051.

Authors 4

Kathrin Michael (first)
Barbara G. Klupp (additional)
Axel Karger (additional)
Thomas C. Mettenleiter (additional)

References 33 Referenced 26

Bartha, A. 1961. Experimental reduction of virulence of Aujeszky's disease virus. Magy. Allatorv. Lapja16:42-45. / Magy. Allatorv. Lapja (1961)
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10.1051/vetres:2001108

Dates

Type	When
Created	18 years, 8 months ago (Dec. 27, 2006, 6:52 p.m.)
Deposited	3 years, 5 months ago (March 5, 2022, 6:26 a.m.)
Indexed	1 year, 1 month ago (July 4, 2024, 4:02 p.m.)
Issued	18 years, 7 months ago (Jan. 15, 2007)
Published	18 years, 7 months ago (Jan. 15, 2007)
Published Print	18 years, 7 months ago (Jan. 15, 2007)

Funders 0

None

BibTeX

@article{Michael_2007, title={Efficient Incorporation of Tegument Proteins pUL46, pUL49, and pUS3 into Pseudorabies Virus Particles Depends on the Presence of pUL21}, volume={81}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.01801-06}, DOI={10.1128/jvi.01801-06}, number={2}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Michael, Kathrin and Klupp, Barbara G. and Karger, Axel and Mettenleiter, Thomas C.}, year={2007}, month=jan, pages={1048–1051} }

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