DOI: 10.1128/jvi.01518-08. Cyclophilin A-Dependent Restriction of Human Immunodeficiency Virus Type 1 Capsid Mutants for Infection of Nondividing Cells

Cyclophilin A-Dependent Restriction of Human Immunodeficiency Virus Type 1 Capsid Mutants for Infection of Nondividing Cells

10.1128/jvi.01518-08

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT Among retroviruses, lentiviruses are unusual in their ability to efficiently infect both dividing and nondividing cells, such as activated T cells and macrophages, respectively. Recent studies implicate the viral capsid protein (CA) as a key determinant of cell-cycle-independent infection by human immunodeficiency virus type 1 (HIV-1). We investigated the effects of the host cell protein cyclophilin A (CypA), which binds to HIV-1 CA, on HIV-1 infection of nondividing cells. The HIV-1 CA mutants A92E, T54A, and R132K were impaired for infection of aphidicolin-arrested HeLa cells, but not HOS cells. The mutants synthesized normal quantities of two-long-terminal-repeat circles in arrested HeLa cells, indicating that the mutant preintegration complexes can enter the nuclei of both dividing and nondividing cells. The impaired infectivity of the CA mutants on both dividing and nondividing HeLa cells was relieved by either pharmacological or genetic disruption of the CypA-CA interaction or by RNA interference-mediated depletion of CypA expression in target cells. A second-site suppressor of the CypA-restricted phenotype also restored the ability of CypA-restricted HIV-1 mutants to infect growth-arrested HeLa cells. These results indicate that CypA-restricted mutants are specifically impaired at a step between nuclear import and integration in nondividing HeLa cells. This study reveals a novel target cell-specific restriction of HIV-1 CA mutants in nondividing cells that is dependent on CypA-CA interactions.

Bibliography

Qi, M., Yang, R., & Aiken, C. (2008). Cyclophilin A-Dependent Restriction of Human Immunodeficiency Virus Type 1 Capsid Mutants for Infection of Nondividing Cells. Journal of Virology, 82(24), 12001â12008.

Authors 3

Mingli Qi (first)
Ruifeng Yang (additional)
Christopher Aiken (additional)

References 56 Referenced 59

Dates

Type	When
Created	16 years, 10 months ago (Oct. 1, 2008, 9:06 p.m.)
Deposited	3 years, 5 months ago (March 5, 2022, 7:09 a.m.)
Indexed	1 year ago (Aug. 4, 2024, 1:18 p.m.)
Issued	16 years, 8 months ago (Dec. 15, 2008)
Published	16 years, 8 months ago (Dec. 15, 2008)
Published Print	16 years, 8 months ago (Dec. 15, 2008)

Funders 0

None

BibTeX

@article{Qi_2008, title={Cyclophilin A-Dependent Restriction of Human Immunodeficiency Virus Type 1 Capsid Mutants for Infection of Nondividing Cells}, volume={82}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.01518-08}, DOI={10.1128/jvi.01518-08}, number={24}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Qi, Mingli and Yang, Ruifeng and Aiken, Christopher}, year={2008}, month=dec, pages={12001–12008} }

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