DOI: 10.1128/jvi.00069-07. In Vitro Analysis of Transneuronal Spread of an Alphaherpesvirus Infection in Peripheral Nervous System Neurons

In Vitro Analysis of Transneuronal Spread of an Alphaherpesvirus Infection in Peripheral Nervous System Neurons

10.1128/jvi.00069-07

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT The neurotropic alphaherpesviruses invade and spread in the nervous system in a directional manner between synaptically connected neurons. Until now, this property has been studied only in living animals and has not been accessible to in vitro analysis. In this study, we describe an in vitro system in which cultured peripheral nervous system neurons are separated from their neuron targets by an isolator chamber ring. Using pseudorabies virus (PRV), an alphaherpesvirus capable of transneuronal spread in neural circuits of many animals, we have recapitulated in vitro all known genetic requirements for retrograde and anterograde transneuronal spread as determined previously in vivo. We show that in vitro transneuronal spread requires intact axons and the presence of the viral proteins gE, gI, and Us9. We also show that transneuronal spread is dependent on the viral glycoprotein gB, which is required for membrane fusion, but not on gD, which is required for extracellular spread. We demonstrate ultrastructural differences between anterograde- and retrograde-traveling virions. Finally, we show live imaging of dynamic fluorescent virion components in axons and postsynaptic target neurons.

Bibliography

Feierbach, B., Bisher, M., Goodhouse, J., & Enquist, L. W. (2007). In Vitro Analysis of Transneuronal Spread of an Alphaherpesvirus Infection in Peripheral Nervous System Neurons. Journal of Virology, 81(13), 6846â6857.

Authors 4

B. Feierbach (first)
M. Bisher (additional)
J. Goodhouse (additional)
L. W. Enquist (additional)

References 33 Referenced 47

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Dates

Type	When
Created	18 years, 4 months ago (April 26, 2007, 1:32 a.m.)
Deposited	3 years, 5 months ago (March 5, 2022, 5:57 a.m.)
Indexed	11 months, 2 weeks ago (Sept. 9, 2024, 10:38 a.m.)
Issued	18 years, 1 month ago (July 1, 2007)
Published	18 years, 1 month ago (July 1, 2007)
Published Print	18 years, 1 month ago (July 1, 2007)

Funders 0

None

BibTeX

@article{Feierbach_2007, title={In Vitro Analysis of Transneuronal Spread of an Alphaherpesvirus Infection in Peripheral Nervous System Neurons}, volume={81}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.00069-07}, DOI={10.1128/jvi.00069-07}, number={13}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Feierbach, B. and Bisher, M. and Goodhouse, J. and Enquist, L. W.}, year={2007}, month=jul, pages={6846–6857} }

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