DOI: 10.1128/jvi.00067-10. Ultrastructural Analysis of Virion Formation and Anterograde Intraaxonal Transport of the Alphaherpesvirus Pseudorabies Virus in Primary Neurons

Ultrastructural Analysis of Virion Formation and Anterograde Intraaxonal Transport of the Alphaherpesvirus Pseudorabies Virus in Primary Neurons

10.1128/jvi.00067-10

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

ABSTRACT A hallmark of alphaherpesviruses is their capacity to be neuroinvasive and establish latent infections in neurons. After primary replication in epithelial cells at the periphery, entry into nerve endings occurs, followed by retrograde transport of nucleocapsids to the nucleus where viral transcription, genome replication, and nucleocapsid formation take place. Translocation of nucleocapsids to the cytoplasm is followed by axonal transport to infect synaptically linked neurons. Two modes of intraaxonal anterograde herpesvirus transport have been proposed: transport of complete, enveloped virions within vesicles (“married model”), and separate transport of capsids and envelopes (“subassembly model”). To assess this in detail for the alphaherpesvirus pseudorabies virus (PrV), we used high-resolution transmission electron microscopy of primary neuronal cultures from embryonic rat superior cervical ganglia after infection with wild-type and gB-deficient PrV. Our data show that intranuclear capsid maturation, nuclear egress and cytoplasmic secondary envelopment occur as in cultured nonpolarized cells (H. Granzow, F. Weiland, A. Jöns, B. G. Klupp, A. Karger, and T. C. Mettenleiter, J. Virol. 71:2072-2082, 1997). PrV virions were present in axons as enveloped particles within vesicles associated with microtubules and apparently leave the neuron by exocytosis primarily at the growth cone. Only a few nonenveloped nucleocapsids were found in the axon. The same picture was observed after infection by phenotypically complemented gB-deficient PrV, which is able to complete only a single round of replication. Our data thus support intraaxonal anterograde transport of enveloped PrV virions within vesicles following the “married model.”

Bibliography

Maresch, C., Granzow, H., Negatsch, A., Klupp, B. G., Fuchs, W., Teifke, J. P., & Mettenleiter, T. C. (2010). Ultrastructural Analysis of Virion Formation and Anterograde Intraaxonal Transport of the Alphaherpesvirus Pseudorabies Virus in Primary Neurons. Journal of Virology, 84(11), 5528â5539.

Authors 7

Christina Maresch (first)
Harald Granzow (additional)
Alexandra Negatsch (additional)
Barbara G. Klupp (additional)
Walter Fuchs (additional)
Jens P. Teifke (additional)
Thomas C. Mettenleiter (additional)

References 44 Referenced 22

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Dates

Type	When
Created	15 years, 5 months ago (March 17, 2010, 8:46 p.m.)
Deposited	3 years, 5 months ago (March 5, 2022, 8:34 a.m.)
Indexed	1 year ago (Aug. 24, 2024, 9:03 a.m.)
Issued	15 years, 3 months ago (June 1, 2010)
Published	15 years, 3 months ago (June 1, 2010)
Published Print	15 years, 3 months ago (June 1, 2010)

Funders 0

None

BibTeX

@article{Maresch_2010, title={Ultrastructural Analysis of Virion Formation and Anterograde Intraaxonal Transport of the Alphaherpesvirus Pseudorabies Virus in Primary Neurons}, volume={84}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.00067-10}, DOI={10.1128/jvi.00067-10}, number={11}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Maresch, Christina and Granzow, Harald and Negatsch, Alexandra and Klupp, Barbara G. and Fuchs, Walter and Teifke, Jens P. and Mettenleiter, Thomas C.}, year={2010}, month=jun, pages={5528–5539} }

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