DOI: 10.1128/jcm.42.7.2996-2999.2004. Evaluation of the Binax NOW, BD Directigen, and BD Directigen EZ Assays for Detection of Respiratory Syncytial Virus

Evaluation of the Binax NOW, BD Directigen, and BD Directigen EZ Assays for Detection of Respiratory Syncytial Virus

10.1128/jcm.42.7.2996-2999.2004

Crossref journal-article

American Society for Microbiology

Journal of Clinical Microbiology (235)

Abstract

ABSTRACT The Binax NOW assay (Binax, Inc., Portland, Maine) and the BD Directigen EZ assay (Becton Dickinson and Company, Sparks, Md.), two new rapid immunoassays for detection of respiratory syncytial virus (RSV), as well as the BD Directigen RSV assay (DRSV) (Becton Dickinson and Company) and direct immunofluorescence staining (DFA) were compared with culture for detection of RSV in fresh specimens from both children and adults during the 2002-2003 respiratory virus season. The majority (95%) of specimens were nasal or nasopharyngeal washes or aspirates. A total of 47 (26%) were culture positive for RSV. The overall sensitivities of DFA ( n = 149), NOW ( n = 118), EZ ( n = 88), and DRSV ( n = 180) compared with culture ( n = 180) were 93, 89, 59, and 77%, respectively. The specificities of DFA, NOW, EZ, and DRSV were 97, 100, 98, and 96%, respectively. However, when results were separated into those from children and those from adults, DFA was the only rapid test adequate for detection of RSV (sensitivity of 100% compared to 0, 0, and 25% for NOW, EZ, and DRSV, respectively) in adults. For children the sensitivities of DFA, NOW, EZ, and DRSV were 93, 94, 72, and 81%. The NOW assay was the most sensitive and specific and the easiest to perform of the kit tests for detecting RSV in children. None of these three rapid kit tests was sensitive for detecting RSV in specimens from adults. DFA remains the rapid method of choice for detecting RSV in the adult population.

Bibliography

Ohm-Smith, M. J., Nassos, P. S., & Haller, B. L. (2004). Evaluation of the Binax NOW, BD Directigen, and BD Directigen EZ Assays for Detection of Respiratory Syncytial Virus. Journal of Clinical Microbiology, 42(7), 2996â2999.

Authors 3

Marilyn J. Ohm-Smith (first)
Patricia S. Nassos (additional)
Barbara L. Haller (additional)

References 9 Referenced 70

10.1128/jcm.31.9.2286-2290.1993
10.1128/jcm.34.7.1649-1653.1996
10.1111/j.1532-5415.1996.tb05641.x
10.1128/CMR.13.3.371
10.1128/jcm.28.5.1021-1025.1990
Keller E. W. 1992. Preparation of cell spots for immunofluorescence p. 8.10.1-8.10.9. In H. D. Isenberg (ed. in chief) Clinical microbiology procedures handbook vol. 2. American Society for Microbiology Washington D.C.
10.1128/JCM.41.7.3407-3409.2003
10.1128/jcm.29.4.824-826.1991
10.1128/jcm.28.3.480-483.1990

Dates

Type	When
Created	21 years, 1 month ago (July 8, 2004, 2:19 p.m.)
Deposited	3 years, 5 months ago (March 4, 2022, 10:21 p.m.)
Indexed	11 months ago (Sept. 22, 2024, 11:41 p.m.)
Issued	21 years, 1 month ago (July 1, 2004)
Published	21 years, 1 month ago (July 1, 2004)
Published Print	21 years, 1 month ago (July 1, 2004)

Funders 0

None

BibTeX

@article{Ohm_Smith_2004, title={Evaluation of the Binax NOW, BD Directigen, and BD Directigen EZ Assays for Detection of Respiratory Syncytial Virus}, volume={42}, ISSN={1098-660X}, url={http://dx.doi.org/10.1128/jcm.42.7.2996-2999.2004}, DOI={10.1128/jcm.42.7.2996-2999.2004}, number={7}, journal={Journal of Clinical Microbiology}, publisher={American Society for Microbiology}, author={Ohm-Smith, Marilyn J. and Nassos, Patricia S. and Haller, Barbara L.}, year={2004}, month=jul, pages={2996–2999} }

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