DOI: 10.1128/jcm.41.6.2341-2347.2003. Distribution of the Secondary Type III Secretion System Locus Found in Enterohemorrhagic <i>Escherichia coli</i> O157:H7 Isolates among Shiga Toxin-Producing <i>E. coli</i> Strains

Distribution of the Secondary Type III Secretion System Locus Found in Enterohemorrhagic Escherichia coli O157:H7 Isolates among Shiga Toxin-Producing E. coli Strains

10.1128/jcm.41.6.2341-2347.2003

Crossref journal-article

American Society for Microbiology

Journal of Clinical Microbiology (235)

Abstract

ABSTRACT The ability of the complete genome sequence of enterohemorrhagic Escherichia coli O157 led to the identification of a 17-kb chromosomal region which contained a type III secretion system gene cluster at min 64.5. This locus contains open reading frames whose amino acid sequences show high degrees of similarity with those of proteins that make up the type III secretion apparatus, which is encoded by the inv-spa-prg locus on a Salmonella SPI-1 pathogenicity island. This locus was designated ETT2 ( E. coli type III secretion 2) and consisted of the epr , epa , and eiv genes. ETT2 was found in enteropathogenic E. coli strains and also in some non-O157 Shiga toxin-producing E. coli (STEC) strains, but most of them contained a truncated portion of ETT2. Most O157 isolates had a complete collection of toxin-encoding genes eae and hlyA and the ETT2 locus, while most O26 strains had toxin-encoding genes eae and hlyA genes but an incomplete ETT2 locus. Thus, an intact copy of ETT2 might mark a pathogenic distinction for particular STEC strains. Therefore, the presence of the ETT2 locus can be used for identification of truly pathogenic STEC strains and for molecular fingerprinting of the epidemic strains in humans and animals.

Bibliography

Makino, S.-I., Tobe, T., Asakura, H., Watarai, M., Ikeda, T., Takeshi, K., & Sasakawa, C. (2003). Distribution of the Secondary Type III Secretion System Locus Found in Enterohemorrhagic Escherichia coli O157:H7 Isolates among Shiga Toxin-Producing E. coli Strains. Journal of Clinical Microbiology, 41(6), 2341â2347.

Authors 7

Sou-Ichi Makino (first)
Toru Tobe (additional)
Hiroshi Asakura (additional)
Masahisa Watarai (additional)
Testuya Ikeda (additional)
Koichi Takeshi (additional)
Chihiro Sasakawa (additional)

References 25 Referenced 46

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Dates

Type	When
Created	22 years, 2 months ago (June 5, 2003, 3:14 p.m.)
Deposited	3 years, 5 months ago (March 4, 2022, 9:25 p.m.)
Indexed	2 months, 2 weeks ago (June 13, 2025, 10:43 a.m.)
Issued	22 years, 3 months ago (June 1, 2003)
Published	22 years, 3 months ago (June 1, 2003)
Published Print	22 years, 3 months ago (June 1, 2003)

Funders 0

None

BibTeX

@article{Makino_2003, title={Distribution of the Secondary Type III Secretion System Locus Found in Enterohemorrhagic Escherichia coli O157:H7 Isolates among Shiga Toxin-Producing E. coli Strains}, volume={41}, ISSN={1098-660X}, url={http://dx.doi.org/10.1128/jcm.41.6.2341-2347.2003}, DOI={10.1128/jcm.41.6.2341-2347.2003}, number={6}, journal={Journal of Clinical Microbiology}, publisher={American Society for Microbiology}, author={Makino, Sou-Ichi and Tobe, Toru and Asakura, Hiroshi and Watarai, Masahisa and Ikeda, Testuya and Takeshi, Koichi and Sasakawa, Chihiro}, year={2003}, month=jun, pages={2341–2347} }

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