DOI: 10.1128/jcm.38.11.4114-4120.2000. Species-Specific Identification of Human Adenoviruses by a Multiplex PCR Assay

Species-Specific Identification of Human Adenoviruses by a Multiplex PCR Assay

10.1128/jcm.38.11.4114-4120.2000

Crossref journal-article

American Society for Microbiology

Journal of Clinical Microbiology (235)

Abstract

ABSTRACT A multiplex PCR assay was developed by using primers to the fiber gene that could differentiate human adenovirus (Ad) species A through F in a single amplification reaction. The assay correctly identified the species of all 49 recognized Ad prototype strains as well as 180 geographically and temporally diverse Ad field isolates. Ad serotype 6 (Ad6) (species C), Ad16 (species B), Ad31 (species A), and Ad40 and Ad41 (species F) could also be distinguished by amplicon size within each respective species. In comparison, a previously described Ad species-specific multiplex PCR assay that used primers to the Ad hexon gene gave equivocal results with several serotypes of species B, whereas our multiplex assay amplified all species B serotypes equally well. Our multiplex PCR assay will permit rapid, accurate, and cost-effective classification of Ad isolates.

Bibliography

Xu, W., McDonough, M. C., & Erdman, D. D. (2000). Species-Specific Identification of Human Adenoviruses by a Multiplex PCR Assay. Journal of Clinical Microbiology, 38(11), 4114â4120.

Authors 3

WanHong Xu (first)
Mike C. McDonough (additional)
Dean D. Erdman (additional)

References 53 Referenced 222

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10.1016/0042-6822(87)90150-4
Cooper R. J. Yeo A. C. Bailey A. S. Tullo A. B. Adenovirus polymerase chain reaction assay for rapid diagnosis of conjunctivitis.Investig. Ophalmol. Vis. Sci.4019999095 / Investig. Ophalmol. Vis. Sci. / Adenovirus polymerase chain reaction assay for rapid diagnosis of conjunctivitis by Cooper R. J. (1999)
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Dates

Type	When
Created	5 years, 8 months ago (Dec. 31, 2019, 12:50 p.m.)
Deposited	3 years, 6 months ago (March 4, 2022, 7:19 p.m.)
Indexed	1 week, 6 days ago (Aug. 23, 2025, 9:49 p.m.)
Issued	24 years, 10 months ago (Nov. 1, 2000)
Published	24 years, 10 months ago (Nov. 1, 2000)
Published Print	24 years, 10 months ago (Nov. 1, 2000)

Funders 0

None

BibTeX

@article{Xu_2000, title={Species-Specific Identification of Human Adenoviruses by a Multiplex PCR Assay}, volume={38}, ISSN={1098-660X}, url={http://dx.doi.org/10.1128/jcm.38.11.4114-4120.2000}, DOI={10.1128/jcm.38.11.4114-4120.2000}, number={11}, journal={Journal of Clinical Microbiology}, publisher={American Society for Microbiology}, author={Xu, WanHong and McDonough, Mike C. and Erdman, Dean D.}, year={2000}, month=nov, pages={4114–4120} }

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  "relation": {},
  "ISSN": [
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    "1098-660X"
  ],
  "subject": [],
  "container-title-short": "J Clin Microbiol",
  "published": {
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        11
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