DOI: 10.1128/jcm.37.1.49-55.1999. <i>Mycobacterium</i> Species Identification and Rifampin Resistance Testing with High-Density DNA Probe Arrays

Mycobacterium Species Identification and Rifampin Resistance Testing with High-Density DNA Probe Arrays

10.1128/jcm.37.1.49-55.1999

Crossref journal-article

American Society for Microbiology

Journal of Clinical Microbiology (235)

Abstract

ABSTRACT Species identification within the genus Mycobacterium and subsequent antibiotic susceptibility testing still rely on time-consuming, culture-based methods. Despite the recent development of DNA probes, which greatly reduce assay time, there is a need for a single platform assay capable of answering the multitude of diagnostic questions associated with this genus. We describe the use of a DNA probe array based on two sequence databases: one for the species identification of mycobacteria (82 unique 16S rRNA sequences corresponding to 54 phenotypical species) and the other for detecting Mycobacterium tuberculosis rifampin resistance ( rpoB alleles). Species identification or rifampin resistance was determined by hybridizing fluorescently labeled, amplified genetic material generated from bacterial colonies to the array. Seventy mycobacterial isolates from 27 different species and 15 rifampin-resistant M. tuberculosis strains were tested. A total of 26 of 27 species were correctly identified as well as all of the rpoB mutants. This parallel testing format opens new perspectives in terms of patient management for bacterial diseases by allowing a number of genetic tests to be simultaneously run.

Bibliography

Troesch, A., Nguyen, H., Miyada, C. G., Desvarenne, S., Gingeras, T. R., Kaplan, P. M., Cros, P., & Mabilat, C. (1999). Mycobacterium Species Identification and Rifampin Resistance Testing with High-Density DNA Probe Arrays. Journal of Clinical Microbiology, 37(1), 49â55.

Authors 8

A. Troesch (first)
H. Nguyen (additional)
C. G. Miyada (additional)
S. Desvarenne (additional)
T. R. Gingeras (additional)
P. M. Kaplan (additional)
P. Cros (additional)
C. Mabilat (additional)

References 31 Referenced 219

10.1126/science.8284673
10.1128/jcm.28.8.1751-1759.1990
10.1126/science.274.5287.610
10.1016/0966-842X(94)90621-1
10.1128/jcm.35.5.1281-1283.1997
10.1002/(SICI)1098-1004(1996)7:3<244::AID-HUMU9>3.0.CO;2-A
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10.1128/JCM.32.4.1095-1098.1994
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Yates M. D. Pozniak A. Uttley A. H. C. Clarke R. Grange J. M. Isolation of environmental mycobacteria from clinical specimens in south-east England: 1973–1993.Int. J. Tuberc. Lung Dis.119977580 / Int. J. Tuberc. Lung Dis. / Isolation of environmental mycobacteria from clinical specimens in south-east England: 1973–1993 by Yates M. D. (1997)
10.1038/358591a0

Dates

Type	When
Created	5 years, 8 months ago (Dec. 31, 2019, 12:37 p.m.)
Deposited	3 years, 6 months ago (March 4, 2022, 6:54 p.m.)
Indexed	3 months, 1 week ago (May 24, 2025, 9:50 p.m.)
Issued	26 years, 8 months ago (Jan. 1, 1999)
Published	26 years, 8 months ago (Jan. 1, 1999)
Published Print	26 years, 8 months ago (Jan. 1, 1999)

Funders 0

None

BibTeX

@article{Troesch_1999, title={Mycobacterium Species Identification and Rifampin Resistance Testing with High-Density DNA Probe Arrays}, volume={37}, ISSN={1098-660X}, url={http://dx.doi.org/10.1128/jcm.37.1.49-55.1999}, DOI={10.1128/jcm.37.1.49-55.1999}, number={1}, journal={Journal of Clinical Microbiology}, publisher={American Society for Microbiology}, author={Troesch, A. and Nguyen, H. and Miyada, C. G. and Desvarenne, S. and Gingeras, T. R. and Kaplan, P. M. and Cros, P. and Mabilat, C.}, year={1999}, month=jan, pages={49–55} }

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