DOI: 10.1128/jb.99.1.142-150.1969. Phospholipid Metabolism in <i>Ferrobacillus ferrooxidans</i>

Phospholipid Metabolism in Ferrobacillus ferrooxidans

10.1128/jb.99.1.142-150.1969

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The lipid composition of the chemoautotroph Ferrobacillus ferrooxidans has been examined. Fatty acids represent 2% of the dry weight of the cells and 86% of the total are extractable with organic solvents. About 25% of the total fatty acids are associated with diacyl phospholipids. Polar carotenoids, the benzoquinone coenzyme Q-8, and most of the fatty acids are present in the neutral lipids. The phospholipids have been identified as phosphatidyl monomethylethanolamine (42%), phosphatidyl glycerol (23%), phosphatidyl ethanolamine (20%), cardiolipin (13%), phosphatidyl choline (1.5%), and phosphatidyl dimethylethanolamine (1%) by chromatography of the diacyl lipids, by chromatography in four systems of the glycerol phosphate esters derived from the lipids by mild alkaline methanolysis, and by chromatographic identification of the products of acid hydrolysis of the esters. No trace of phosphatidylserine (PS), glycerolphosphorylserine, or serine could be detected in the lipid extract or in derivatives of that extract. This casts some doubt on the postulated involvement of PS in iron metabolism. After growth in the presence of 14 C and 32 P, there was essentially no difference in the turnover of either isotope in the glycerolphosphate ester derived from each lipid in cells grown at p H 1.5 or 3.5.

Bibliography

Short, S. A., White, D. C., & Aleem, M. I. H. (1969). Phospholipid Metabolism in Ferrobacillus ferrooxidans. Journal of Bacteriology, 99(1), 142â150.

Authors 3

Steven A. Short (first)
David C. White (additional)
M. I. H. Aleem (additional)

References 21 Referenced 56

Barridge , J. K. , and J. M. Shively . 1968 . Phospholipids of the thiobacilli . J. Bacteriol. 95 : 2182 - 2185 . (10.1128/jb.95.6.2182-2185.1968) / J. Bacteriol. / Phospholipids of the thiobacilli by Barridge J. K. (1968)
Bligh , E. G. , and W. J. Dyer . 1959 . A rapid method of total lipid extraction and purification . Can. J. Biochem. Physiol. 37 : 911 - 917 . (10.1139/o59-099) / Can. J. Biochem. Physiol. / A rapid method of total lipid extraction and purification by Bligh E. G. (1959)
Blaylock , B. A. , and A. Nelson . 1963 . Electron transport systems of the chemoautotroph Ferrobacillus ferrooxidans. I. Cytochrome c-containing iron oxidase . J. Biol. Chem. 238 : 3453 - 3462 . (10.1016/S0021-9258(18)48688-7) / J. Biol. Chem. / Electron transport systems of the chemoautotroph Ferrobacillus ferrooxidans. I. Cytochrome c-containing iron oxidase by Blaylock B. A. (1963)
Bremer , J. , P. H. Figard , and D. M. Greenberg . 1960 . The biosynthesis of choline and its relation to phospholipid metabolism . Biochim. Biophys. Acta 43 : 477 - 488 . (10.1016/0006-3002(60)90470-4) / Biochim. Biophys. Acta / The biosynthesis of choline and its relation to phospholipid metabolism by Bremer J. (1960)
Din G. A. I. Suzuki and H. Lees. 1967. Ferrous iron oxidation by Ferrobacillus ferrooxidans. Purification and 150 SHORT WHITE AND ALEEM (10.1139/o67-183)
Dittmer , J. C. , and R. L. Lester . 1964 . A simple, specific spray for the detection of phospholipids on thin layer chromatograms . J. Lipid Res. 5 : 126 - 128 . (10.1016/S0022-2275(20)40272-X) / J. Lipid Res. / A simple, specific spray for the detection of phospholipids on thin layer chromatograms by Dittmer J. C. (1964)
Hokin M. R. and L. E. Hokin. p. 423-434. In R. M. C. Dawson and D. N. Rhodes (ed.) Metabolism and physiological significance of lipids. John Wiley and Sons London 1965.
Korczynski , M. S. , A. D. Agate , and D. G. Lundgren . 1967 . Phospholipids from the chemoautotroph Ferrobacillus ferrooxidans . Biochem. Biophys. Res. Commun. 29 : 457 - 462 . (10.1016/0006-291X(67)90505-0) / Biochem. Biophys. Res. Commun. / Phospholipids from the chemoautotroph Ferrobacillus ferrooxidans by Korczynski M. S. (1967)
Lane , E. S. 1965 . Thin layer chromatography of long-chain tertiary amines and related compounds . J. Chromatog. 18 : 426 - 430 . (10.1016/S0021-9673(01)80394-0) / J. Chromatog. / Thin layer chromatography of long-chain tertiary amines and related compounds by Lane E. S. (1965)
Lester , R. L. , and T. Ramasarma . 1959 . Chromatography of the coenzyme Q family of compounds on silicone-impregnated paper . J. Biol. Chem. 234 : 672 - 676 . (10.1016/S0021-9258(18)70267-6) / J. Biol. Chem. / Chromatography of the coenzyme Q family of compounds on silicone-impregnated paper by Lester R. L. (1959)
Novak , M. 1965 . Colorimetric ultramicro method for the determination of free fatty acids . J. Lipid Res. 6 : 431 - 433 . (10.1016/S0022-2275(20)39315-9) / J. Lipid Res. / Colorimetric ultramicro method for the determination of free fatty acids by Novak M. (1965)
Remsen , C. , and D. G. Lundgren . 1966 . Electron microscopy of the cell envelope of Ferrobacillus ferrooxidans prepared by freeze-etching and chemical fixation techniques . J. Bacteriol. 92 : 1765 - 1771 . (10.1128/JB.92.6.1765-1771.1966) / J. Bacteriol. / Electron microscopy of the cell envelope of Ferrobacillus ferrooxidans prepared by freeze-etching and chemical fixation techniques by Remsen C. (1966)
Salton , M. R. J. 1967 . Isolation and characterization of bacterial membranes . Trans. N. Y. Acad. Sci. Ser. II 29 : 764 - 781 . (10.1111/j.2164-0947.1967.tb02300.x) / Trans. N. Y. Acad. Sci. Ser. II / Isolation and characterization of bacterial membranes by Salton M. R. J. (1967)
Schmidt , G. , M. J. Bessman , and S. J. Tannhauser . 1953 . The hydrolysis of L-a-glycerolphosphorylethanolamine . J. Biol. Chem. 203 : 849 - 853 . (10.1016/S0021-9258(19)52353-5) / J. Biol. Chem. / The hydrolysis of L-a-glycerolphosphorylethanolamine by Schmidt G. (1953)
Shively , J. M. , and A. A. Benson . 1967 . Phospholipids of Thiobacillus thiooxldans . J. Bacteriol. 94 : 1679 - 1683 . (10.1128/JB.94.5.1679-1683.1967) / J. Bacteriol. / Phospholipids of Thiobacillus thiooxldans by Shively J. M. (1967)
Silverman , M. P. , and D. G. Lundgren . 1959 . Studies on the chemoautotrophic iron bacterium Ferrobacillus ferrooxidans. I. An improved medium and a harvesting procedure for securing high cell yields . J. Bacteriol. 77 : 642 - 647 . (10.1128/jb.77.5.642-647.1959) / J. Bacteriol. / Studies on the chemoautotrophic iron bacterium Ferrobacillus ferrooxidans. I. An improved medium and a harvesting procedure for securing high cell yields by Silverman M. P. (1959)
White , D. C. 1968 . Lipid composition of the electron transport membrane of Haemophilus paralnfluenzae . J. Bacteriol. 96 : 1159 - 1170 . (10.1128/jb.96.4.1159-1170.1968) / J. Bacteriol. / Lipid composition of the electron transport membrane of Haemophilus paralnfluenzae by White D. C. (1968)
White , D. C. , and R. H. Cox . 1967 . Identification and localization of the fatty acids in Haemophilus parainfluenzae . J. Bacteriol. 93 : 1079 - 1088 . (10.1128/JB.93.3.1079-1088.1967) / J. Bacteriol. / Identification and localization of the fatty acids in Haemophilus parainfluenzae by White D. C. (1967)
White , D. C. , and F. E. Frerman . 1967 . Extraction, characterization, and cellular localization of the lipids of Staphylococcus aureus . J. Bacteriol. 94 : 1854 - 1867 . (10.1128/JB.94.6.1854-1867.1967) / J. Bacteriol. / Extraction, characterization, and cellular localization of the lipids of Staphylococcus aureus by White D. C. (1967)
White D. C. and A. N. Tucker. 1969. Phospholipid metabolism during bacterial growth. J. Lipid Res.- 10:220-233. (10.1016/S0022-2275(20)42672-0)
Wuthier , R. E. 1966 . Two-dimensional chromatography on silica gel-loaded paper for the microanalysis of polar lipids . J. Lipid Res. 7 : 544 - 550 . (10.1016/S0022-2275(20)39265-8) / J. Lipid Res. / Two-dimensional chromatography on silica gel-loaded paper for the microanalysis of polar lipids by Wuthier R. E. (1966)

Dates

Type	When
Created	5 years, 8 months ago (Jan. 4, 2020, 9:18 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 12:54 p.m.)
Indexed	2 months, 1 week ago (June 25, 2025, 8:12 p.m.)
Issued	56 years, 2 months ago (July 1, 1969)
Published	56 years, 2 months ago (July 1, 1969)
Published Print	56 years, 2 months ago (July 1, 1969)

Funders 0

None

BibTeX

@article{Short_1969, title={Phospholipid Metabolism in Ferrobacillus ferrooxidans}, volume={99}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.99.1.142-150.1969}, DOI={10.1128/jb.99.1.142-150.1969}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Short, Steven A. and White, David C. and Aleem, M. I. H.}, year={1969}, month=jul, pages={142–150} }

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