DOI: 10.1128/jb.99.1.136-141.1969. Interaction of <i>Bdellovibrio bacteriovorus</i> and Host Bacteria II. Intracellular Growth and Development of <i>Bdellovibrio bacteriovorus</i> in Liquid Cultures

Interaction of Bdellovibrio bacteriovorus and Host Bacteria II. Intracellular Growth and Development of Bdellovibrio bacteriovorus in Liquid Cultures

10.1128/jb.99.1.136-141.1969

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The intracellular life cycle of Bdellovibrio bacteriovorus 109 growing on Escherichia coli in a dilute nutrient medium exhibits a period of constant infective titer while the parasite grows and elongates inside the host cell. This period is terminated after 2 to 4 hr, and the number of the plaque-forming units in the culture rises rapidly to as much as six times the initial titer. The growth pattern of Bdellovibrio is similar with actively growing or resting host cells, or with host cells killed by ultraviolet irradiation or by heating at 70 C. The yield of B. bacteriovorus strain 109 in two-membered cultures with E. coli B depends on the host concentration and may reach 7.5 × 10 10 cells per ml. Penicillin, which has no effect on the attachment and penetration of Bdellovibrio , inhibits its multiplication.

Bibliography

Varon, M., & Shilo, M. (1969). Interaction of Bdellovibrio bacteriovorus and Host Bacteria II. Intracellular Growth and Development of Bdellovibrio bacteriovorus in Liquid Cultures. Journal of Bacteriology, 99(1), 136â141.

Authors 2

Mazal Varon (first)
M. Shilo (additional)

References 10 Referenced 46

Burger , A. , G. Drews , and R. Ladwig . 1968 . Wirtskreis und Infektionscyclus eines neu isolierten Bdellovibrio bacterlovorus-stammes . Arch. Mikrobiol. 61 : 261 - 279 . (10.1007/BF00446612) / Arch. Mikrobiol. / Wirtskreis und Infektionscyclus eines neu isolierten Bdellovibrio bacterlovorus-stammes by Burger A. (1968)
Lepine , P. , A. Gu 6lin, J. Sisman , and D. Lamblin . 1967 . Etude au microscope 61ectrionique de la Iyse de Salmonella par Bdellovibrio bacterlovorus . Compt. Rend. 264 : 2957 - 2960 . / Compt. Rend. / Etude au microscope 61ectrionique de la Iyse de Salmonella par Bdellovibrio bacterlovorus by Lepine P. (1967)
Scherff , R. H. , J. E. Devay , and T. W. Carroll . 1966 . Ultrastructure of host-parasite relationships involving reproduction of Bdellovibrio bacteriovorus in host bacteria . Phytopathology 56 : 627 - 632 . / Phytopathology / Ultrastructure of host-parasite relationships involving reproduction of Bdellovibrio bacteriovorus in host bacteria by Scherff R. H. (1966)
Seidler , R. J. , and M. P. Starr . 1969 . Factors affecting the intracellular parasitic growth of Bdellovibrio bacteriovorus developing within Escherichia coli . J. Bacteriol. 97 : 912 - 923 . (10.1128/JB.97.2.912-923.1969) / J. Bacteriol. / Factors affecting the intracellular parasitic growth of Bdellovibrio bacteriovorus developing within Escherichia coli by Seidler R. J. (1969)
Shilo , M. , and B. Bruff . 1965 . Lysis of gram-negative bacteria by host-independent ectoparasitic Bdellovibrio bacteriovorus isolates . J. Gen. Microbiol. 40 : 317 - 328 . (10.1099/00221287-40-3-317) / J. Gen. Microbiol. / Lysis of gram-negative bacteria by host-independent ectoparasitic Bdellovibrio bacteriovorus isolates by Shilo M. (1965)
Simpson , F. J. , and J. Robinson . 1968 . Some energy producing systems in Bdellovibrio bacteriovorus strain 6-5-S . Can. J. Biochem. 46 : 865 - 873 . (10.1139/o68-129) / Can. J. Biochem. / Some energy producing systems in Bdellovibrio bacteriovorus strain 6-5-S by Simpson F. J. (1968)
Starr , M. P. , and N. L. Baigent . 1966 . Parasitic interaction of Bdellovibrio bacteriovorus with other bacteria . J. Bacteriol. 91 : 2006 - 2017 . (10.1128/JB.91.5.2006-2017.1966) / J. Bacteriol. / Parasitic interaction of Bdellovibrio bacteriovorus with other bacteria by Starr M. P. (1966)
Stent G. S. 1963. Molecular biology of bacterial viruses. W. H. Freeman and Co. San Francisco.
Varon , M. , and M. Shilo . 1968 . Interaction of Bdellovibrio bacteriovorus and host bacteria. L. Kinetic studies of attachment and invasion of Escherichia coil B by Bdellovibrio bacteriovorus . J. Bacteriol 95 : 744 - 753 . (10.1128/jb.95.3.744-753.1968) / J. Bacteriol / Interaction of Bdellovibrio bacteriovorus and host bacteria. L. Kinetic studies of attachment and invasion of Escherichia coil B by Bdellovibrio bacteriovorus by Varon M. (1968)
Wollman , E. L. , and G. S. Stent . 1952 . Studies on activation of T4 bacteriophage by cofactor. IV. Nascent activity . Biochim. Biophys. Acta 9 : 538 - 550 . (10.1016/0006-3002(52)90204-7) / Biochim. Biophys. Acta / Studies on activation of T4 bacteriophage by cofactor. IV. Nascent activity by Wollman E. L. (1952)

Dates

Type	When
Created	5 years, 7 months ago (Jan. 4, 2020, 9:18 p.m.)
Deposited	4 years, 2 months ago (May 31, 2021, 10:42 a.m.)
Indexed	21 hours, 59 minutes ago (Aug. 28, 2025, 8:33 a.m.)
Issued	56 years, 1 month ago (July 1, 1969)
Published	56 years, 1 month ago (July 1, 1969)
Published Print	56 years, 1 month ago (July 1, 1969)

Funders 0

None

BibTeX

@article{Varon_1969, title={Interaction of Bdellovibrio bacteriovorus and Host Bacteria II. Intracellular Growth and Development of Bdellovibrio bacteriovorus in Liquid Cultures}, volume={99}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.99.1.136-141.1969}, DOI={10.1128/jb.99.1.136-141.1969}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Varon, Mazal and Shilo, M.}, year={1969}, month=jul, pages={136–141} }

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