DOI: 10.1128/jb.98.2.415-420.1969. Fatty Acid Desaturase Mutants of <i>Saccharomyces cerevisiae</i>

Fatty Acid Desaturase Mutants of Saccharomyces cerevisiae

10.1128/jb.98.2.415-420.1969

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Genetic and biochemical analyses were conducted on fatty acid mutants of yeast deficient for Δ 9 -desaturase activity in the production of palmitoleate and oleate. Two genetic loci were observed and two others are inferred; three of these were represented by respiratory-deficient (petite) strains. All strains were incapable of converting palmitate to palmitoleate and stearate to oleate whether the direct precursor or acetate was followed. All strains were capable of acylating both de novoproduced fatty acids and oleate taken up from the medium into phospholipids and neutral lipids. Two revertants were analyzed which differed in their ability to produce palmitoleate and oleate.

Bibliography

Keith, A. D., Resnick, M. R., & Haley, A. B. (1969). Fatty Acid Desaturase Mutants of Saccharomyces cerevisiae. Journal of Bacteriology, 98(2), 415â420.

Authors 3

A. D. Keith (first)
M. R. Resnick (additional)
A. B. Haley (additional)

References 22 Referenced 70

Andreasen , A. A. , and T. J. B. Stier . 1953 . Anaerobic nutrients for yeast . J. Cell. Comp. Physiol. 41 : 23 - 26 . / J. Cell. Comp. Physiol. / Anaerobic nutrients for yeast by Andreasen A. A. (1953)
Bloomfield , D. K , and K. Bloch . 1960 . The formation of Ag-unsaturated fatty acids . J. Biol. Chem. 235 : 337 - 345 . (10.1016/S0021-9258(18)69525-0) / J. Biol. Chem. / The formation of Ag-unsaturated fatty acids by Bloomfield D. K (1960)
Bottcher , C. J. F. , F. P. Woodford , E. Boelsma -van Houte, and C. M. van Gent . 1959 . Methods for the analysis of lipids extracted from human arteries and other tissues . Rec. Trav. Chim. Pays-Bas 78 : 795 - 815 . / Rec. Trav. Chim. Pays-Bas / Methods for the analysis of lipids extracted from human arteries and other tissues by Bottcher C. J. F. (1959)
Broquist , H. P. , and E. E. Snell . 1951 . Biotin and bacterial growth. I. Relation to aspartate, oleate, and carbon dioxide . J. Biol. Chem. 188 : 431 - 444 . / J. Biol. Chem. / Biotin and bacterial growth. I. Relation to aspartate, oleate, and carbon dioxide by Broquist H. P. (1951)
Demain , A. L. , D. Hendlin , and J. A. Newkirk . 1959 . Role of fatty acids in the growth stimulation of Sarcina species by vitamin-free casein digests . J. Bacteriol. 78 : 839 - 843 . (10.1128/JB.78.6.839-843.1959) / J. Bacteriol. / Role of fatty acids in the growth stimulation of Sarcina species by vitamin-free casein digests by Demain A. L. (1959)
Folch , J. , M. Lees , and G. H. Sloane-Stanley . 1957 . A simple method for the isolation and purification of total lipides from animal tissues . J. Biol. Chem. 226 : 497 - 509 . (10.1016/S0021-9258(18)64849-5) / J. Biol. Chem. / A simple method for the isolation and purification of total lipides from animal tissues by Folch J. (1957)
Hofmann , K. , W. M. O'Leary , C. W. Yoho , and T. Liu . 1959 . Further observations on lipide stimulation of bacterial growth . J. Biol. Chem. 234 : 1672 - 1677 . (10.1016/S0021-9258(18)69905-3) / J. Biol. Chem. / Further observations on lipide stimulation of bacterial growth by Hofmann K. (1959)
Johnston , J. R. , and R. K. Mortimer . 1959 . Use of snail digestive juice in isolation of yeast spore tetrads . J. Bacteriol. 78 : 292 . (10.1128/JB.78.2.292-292.1959) / J. Bacteriol. / Use of snail digestive juice in isolation of yeast spore tetrads by Johnston J. R. (1959)
Keith , A. D. , A. S. Waggoner , and 0. H. Griffith . 1968 . Spin-labeled mitochondrial lipids in Neurospora crassa . Proc. Nat. Acad. Sci. U.S.A. 61 : 819 - 826 . (10.1073/pnas.61.3.819) / Proc. Nat. Acad. Sci. U.S.A. / Spin-labeled mitochondrial lipids in Neurospora crassa by Keith A. D. (1968)
Klein , P. D. , and W. J. Eisler , Jr. 1966 . An improved description of separation and performance capabilities of liquid scintillation counters used in dual isotope studies . Anal. Chem. 38 : 1453 - 1457 . (10.1021/ac60243a003) / Anal. Chem. / An improved description of separation and performance capabilities of liquid scintillation counters used in dual isotope studies by Klein P. D. (1966)
Kitay , E. , and E. E. Snell . 1950 . Some additional nutritional requirements of certain lactic acid bacteria . J. Bacteriol. 60 : 49 - 56 . (10.1128/JB.60.1.49-56.1950) / J. Bacteriol. / Some additional nutritional requirements of certain lactic acid bacteria by Kitay E. (1950)
Lein , J. , and P. S. Lein . 1949 . Studies on a Neurospora mutant requiring unsaturated fatty acids for growth . J. Bacteriol. 58 : 595 - 599 . (10.1128/jb.58.5.595-599.1949) / J. Bacteriol. / Studies on a Neurospora mutant requiring unsaturated fatty acids for growth by Lein J. (1949)
Meyer , F. , R. J. Light , and K. Bloch . 1963 . Fattyacidmetabolism in anaerobic yeast . Biochim. Biophys. Acta 1 : 415 - 421 . / Biochim. Biophys. Acta / Fattyacidmetabolism in anaerobic yeast by Meyer F. (1963)
Nagai , J. , and K. Bloch . 1968 . Enzymatic desaturation of stearyl acyl carrier protein . J. Biol. Chem. 243 : 4626 - 4633 . (10.1016/S0021-9258(18)93235-7) / J. Biol. Chem. / Enzymatic desaturation of stearyl acyl carrier protein by Nagai J. (1968)
Nieman , C. 1954 . Influence of trace amounts of fatty acids on the growth of microorganisms . Bacteriol. Rev. 18 : 147 - 163 . (10.1128/BR.18.2.147-163.1954) / Bacteriol. Rev. / Influence of trace amounts of fatty acids on the growth of microorganisms by Nieman C. (1954)
Resnick , M. A. , and R. K. Mortimer . 1966 . Unsaturated fatty acid mutants of Saccharomyces cerevisiae . J. Bacteriol. 92 : 597 - 600 . (10.1128/JB.92.3.597-600.1966) / J. Bacteriol. / Unsaturated fatty acid mutants of Saccharomyces cerevisiae by Resnick M. A. (1966)
Rodwell A. 1968. Fatty-acid composition of mycoplasma 420 KEITH RESNICK AND HALEY
Sherman , F. 1968 . Respiration-deficient mutants of yeast . I. Genetics 48 : 375 - 385 . (10.1093/genetics/48.3.375) / I. Genetics / Respiration-deficient mutants of yeast by Sherman F. (1968)
Silbert , D. F. , F. Ruch , and P. R. Vagelos . 1968 . Fatty acid replacements in a fatty acid auxotroph of Escherichia coli . J. Bacteriol. 95 : 1658 - 1665 . (10.1128/jb.95.5.1658-1665.1968) / J. Bacteriol. / Fatty acid replacements in a fatty acid auxotroph of Escherichia coli by Silbert D. F. (1968)
Silbert D. F. and P. R. Vagelos. 1967. Fatty acid mutant of J. BACTERIOL.
Williams , V. R. , and E. A. Fieger . 1946 . Oleic acid as a growth * stimulant for Lactobacillus casel . J. Biol. Chem. 166 : 335 - 343 . (10.1016/S0021-9258(17)35011-1) / J. Biol. Chem. / Oleic acid as a growth * stimulant for Lactobacillus casel by Williams V. R. (1946)
Williams , W. L. , H. P. Broquist , and E. E. Snell . 1947 . Oleic acid and related compounds as growth factors for lactic acid bacteria . J. Biol. Chem. 170 : 619 - 630 . (10.1016/S0021-9258(17)30843-8) / J. Biol. Chem. / Oleic acid and related compounds as growth factors for lactic acid bacteria by Williams W. L. (1947)

Dates

Type	When
Created	5 years, 8 months ago (Jan. 4, 2020, 9:16 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 12:53 p.m.)
Indexed	2 days, 22 hours ago (Sept. 4, 2025, 10:23 a.m.)
Issued	56 years, 4 months ago (May 1, 1969)
Published	56 years, 4 months ago (May 1, 1969)
Published Print	56 years, 4 months ago (May 1, 1969)

Funders 0

None

BibTeX

@article{Keith_1969, title={Fatty Acid Desaturase Mutants of Saccharomyces cerevisiae}, volume={98}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.98.2.415-420.1969}, DOI={10.1128/jb.98.2.415-420.1969}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Keith, A. D. and Resnick, M. R. and Haley, A. B.}, year={1969}, month=may, pages={415–420} }

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    "date-parts": [
      [
        1969,
        5
      ]
    ]
  },
  "assertion": [
    {
      "value": "2021-02-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}