DOI: 10.1128/jb.97.3.1411-1418.1969. Growth of Large Plasmodia of the Myxomycete <i>Physarum polycephalum</i>

Growth of Large Plasmodia of the Myxomycete Physarum polycephalum

10.1128/jb.97.3.1411-1418.1969

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

A method has been developed for growing Physarum polycephalum plasmodia that are 8 to 10 times larger than those obtained in the petri dish cultures used by Nygaard, Guttes, and Rusch. In the large-scale procedure, plasmodia were grown in metal trays on a membrane supported by filter paper on stainless-steel screen. Plasmodia were started from a ring of inoculum to allow inward and outward migration and were incubated on a rocker so that nutrient medium would flow back and forth, wetting the undersurface of the plasmodium. Rocker and petri dish cultures had similar growth characteristics: (i) the interphase time between mitoses I and II and between II and III was about 8 hr; (ii) ribonucleic acid and protein increased essentially logarithmically throughout the cell cycle; and (iii) deoxyribonucleic acid increased only during early interphase and it doubled in approximately 3 hr after each mitosis. Rocker cultures were not as nearly synchronous as petri dish cultures and had a range in metaphase time (at mitosis III) within individual plasmodia of 15 to 45 min, as compared with 5 to 10 min in petri dish cultures.

Bibliography

Mohberg, J., & Rusch, H. P. (1969). Growth of Large Plasmodia of the Myxomycete Physarum polycephalum. Journal of Bacteriology, 97(3), 1411â1418.

Authors 2

Joyce Mohberg (first)
Harold P. Rusch (additional)

References 12 Referenced 78

Burton , K. 1956 . A study of the conditions and mechanisms of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid . Biochem. J. 62 : 315 - 322 . (10.1042/bj0620315) / Biochem. J. / A study of the conditions and mechanisms of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid by Burton K. (1956)
Ceriotti , G. 1955 . Determination of nucleic acids in animnal tissues . J. Biol. Chem. 214 : 59 - 70 . (10.1016/S0021-9258(18)70943-5) / J. Biol. Chem. / Determination of nucleic acids in animnal tissues by Ceriotti G. (1955)
Cummins , J. E. , J. C. Blomquist , and H. P. Rusch . 1966 . Anaphase delay after inhibition of protein synthesis between late prophase and prometaphase . Science 154 : 1343 - 1344 . (10.1126/science.154.3754.1343) / Science / Anaphase delay after inhibition of protein synthesis between late prophase and prometaphase by Cummins J. E. (1966)
Daniel J. W. and H. H. Baldwin. 1964. Methods of culture for plasmodial myxomycetes p. 9-41. In D. M. Prescott (ed.) Methods in cell physiology vol. 1. Academic Press Inc. New York. (10.1016/S0091-679X(08)62084-1)
Guttes E. and S. Guttes. 1964. Mitotic synchrony in the plasmodia of Physarum polycephalum and mitotic synchronization by coalescence of microplasmodia p. 43-54. In D. M. Prescott (ed.) Methods in cell physiology vol. 1. Academic Press Inc. New York. (10.1016/S0091-679X(08)62085-3)
Guttes , E. , S. Guttes , and H. P. Rusch . 1961 . Morphological observations on growth and differentiation of Physarum polycephalum grown in pure culture . Develop. Biol. 3 : 588 - 614 . (10.1016/0012-1606(61)90034-3) / Develop. Biol. / Morphological observations on growth and differentiation of Physarum polycephalum grown in pure culture by Guttes E. (1961)
Guttes , S. , E. Guttes , and R. A. Elis . 1968 . Electron microscope study of mitosis in Physarum polycephalum . J. Ultrastruct. Res. 22 : 508 - 529 . (10.1016/S0022-5320(68)90038-5) / J. Ultrastruct. Res. / Electron microscope study of mitosis in Physarum polycephalum by Guttes S. (1968)
Lowry , 0. H. , N. J. Rosebrough , A. L. Farr , and R. J. Randall . 1951 . Protein measurement with the Folin phenol reagent . J. Biol. Chem. 193 : 265 - 275 . (10.1016/S0021-9258(19)52451-6) / J. Biol. Chem. / Protein measurement with the Folin phenol reagent by Lowry 0. (1951)
Mittermayer , C. , R. Braun , T. G. Chayka , and H. P. Rusch . 1966 . Polysome patterns and protein synthesis during the mitotic cycle of Physarum polycephalum . Nature 210 : 1133 - 1137 . (10.1038/2101133a0) / Nature / Polysome patterns and protein synthesis during the mitotic cycle of Physarum polycephalum by Mittermayer C. (1966)
Mittermayer , C. , R. Braun , and H. P. Rusch . 1965 . The effect of actinomycin D on the timing of mitosis in Physarum polycephalum . Exptl. Cell Res. 38 : 33 - 41 . (10.1016/0014-4827(65)90424-6) / Exptl. Cell Res. / The effect of actinomycin D on the timing of mitosis in Physarum polycephalum by Mittermayer C. (1965)
Nygaard , 0. F. , S. Guttes , and H. P. Rusch . 1960 . Nucleic acid metabolism in a slime mold with synchronous mitosis . Biochim. Biophys. Acta 38 : 298 - 306 . (10.1016/0006-3002(60)91245-2) / Biochim. Biophys. Acta / Nucleic acid metabolism in a slime mold with synchronous mitosis by Nygaard 0. (1960)
Sachsenmaier , W. , and H. P. Rusch . 1964 . The effect of 5- fluoro-2'-deoxyuridine on synchronous mitosis in Physarum polycephalum . Exptl. Cell Res. 36 : 124 - 133 . (10.1016/0014-4827(64)90166-1) / Exptl. Cell Res. / The effect of 5- fluoro-2'-deoxyuridine on synchronous mitosis in Physarum polycephalum by Sachsenmaier W. (1964)

Dates

Type	When
Created	5 years, 8 months ago (Jan. 4, 2020, 9:15 p.m.)
Deposited	4 years ago (Sept. 3, 2021, 10:57 p.m.)
Indexed	1 year, 1 month ago (July 10, 2024, 7:15 a.m.)
Issued	56 years, 6 months ago (March 1, 1969)
Published	56 years, 6 months ago (March 1, 1969)
Published Print	56 years, 6 months ago (March 1, 1969)

Funders 0

None

BibTeX

@article{Mohberg_1969, title={Growth of Large Plasmodia of the Myxomycete Physarum polycephalum}, volume={97}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.97.3.1411-1418.1969}, DOI={10.1128/jb.97.3.1411-1418.1969}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Mohberg, Joyce and Rusch, Harold P.}, year={1969}, month=mar, pages={1411–1418} }

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