DOI: 10.1128/jb.95.4.1439-1449.1968. Nutritional Factors Influencing the Development of Competence in the <i>Bacillus subtilis</i> Transformation System

Nutritional Factors Influencing the Development of Competence in the Bacillus subtilis Transformation System

10.1128/jb.95.4.1439-1449.1968

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Cultures of Bacillus subtilis developed competence for the uptake of deoxyribonucleic acid in a chemically defined medium with a predictable, reproducible pattern. The gross effects of individual amino acids were determined. Seven amino acids, most of which are reported to be major components of the cell wall, were shown to impair the development of maximal levels of competence. When the synthetic growth medium was supplemented with a mixture of the nine amino acids which we found to stimulate the development of competence, the level of transfection was increased to 10 to 15% of the population. The actual level of competence in these populations was assayed by transformation of unlinked bacterial markers and by two different transfection assays. The results indicate that calculations from cotransfer of unlinked markers overestimates the degree of competence in highly competent populations of B. subtilis , whereas the number of plaques obtained in transfection is an under-estimate of the actual level of competence. The results are interpreted to indicate that neither method of analysis gives a true estimate of the competent population, but that more than 80% of the cells may be competent.

Bibliography

Wilson, G. A., & Bott, K. F. (1968). Nutritional Factors Influencing the Development of Competence in the Bacillus subtilis Transformation System. Journal of Bacteriology, 95(4), 1439â1449.

Authors 2

Gary A. Wilson (first)
Kenneth F. Bott (additional)

References 15 Referenced 178

ANAGNOSTOPOULOS , C. , AND J. SPIZIZEN. 1961 . Requirements for transformation in Bacillus subtilis . J. Bacteriol. 81 : 741 - 746 . (10.1128/JB.81.5.741-746.1961) / J. Bacteriol. / Requirements for transformation in Bacillus subtilis by ANAGNOSTOPOULOS C. (1961)
Borr, K. F., AND G. A. WILSON. 1967 . Development of competence in the Bacillus subtilis transformation system . J. Bacteriol. 94 : 562 - 570 . (10.1128/JB.94.3.562-570.1967) / J. Bacteriol. / Development of competence in the Bacillus subtilis transformation system by G. A. (1967)
DEMEREC , M., E. A. ADELBERG , A. J. CLARK , A ND P . E. HARTMAN. 1966 . A proposal for a uniform nomenclature in bacterial genetics . Genetics 54 : 61 - 76 . (10.1093/genetics/54.1.61) / Genetics / A proposal for a uniform nomenclature in bacterial genetics by DEMEREC M., E. (1966)
Fox, M. S., AND R. D. HOTCHKISS. 1957 . Initiation of bacterial transformation . Nature 179 : 1322 - 1325 . (10.1038/1791322a0) / Nature / Initiation of bacterial transformation by R. D. (1957)
GOODGAL , S. H. , AND R. M. HERRIOTF. 1961 . Studies on transformation of Haemophilus influenzae . I. Competence. J. Gen. Physiol. 44 : 1201 - 1227 . (10.1085/jgp.44.6.1201) / I. Competence. J. Gen. Physiol. / Studies on transformation of Haemophilus influenzae by GOODGAL S. H. (1961)
KAMMEN , H. 0., R. H. BELOFF , A ND E . S. CANELLAKIS. 1966 . Transformation in Bacillus subtilis. I. Role of amino acids in stabilization of transformants . Biochim. Biophys. Acta 123 : 39 - 55 . / Biochim. Biophys. Acta / Transformation in Bacillus subtilis. I. Role of amino acids in stabilization of transformants by KAMMEN H. (1966)
KAMMEN , H. 0., R. J. WOJNAR , A ND E . S. CANELLAKIS. 1966 . Transformation in Bacillus subtilis. II. The development and maintenance of the competent state . Biochim. Biophys. Acta 123 : 56 - 65 . (10.1016/0005-2787(66)90158-4) / Biochim. Biophys. Acta / Transformation in Bacillus subtilis. II. The development and maintenance of the competent state by KAMMEN H. (1966)
S. NESTER E. W. AND B. A. D. STOCKER. 1963. Biosynthetic latency in early stages of deoxyri-1449
SPIZIZEN , J. 1959 . Genetic activity of deoxyribonucleic acid in reconstitution of biosynthetic pathways . Federation Proc. 18 : 957 - 965 . / Federation Proc. / Genetic activity of deoxyribonucleic acid in reconstitution of biosynthetic pathways by SPIZIZEN J. (1959)
SPIZIZEN , J., B. E. REILLY , A ND A . H. EVANS. 1966 . Microbial transformation and transfec: tion . Ann. Rev. Microbiol. 20 : 371 - 400 . (10.1146/annurev.mi.20.100166.002103) / Ann. Rev. Microbiol. / Microbial transformation and transfec: tion by SPIZIZEN J., B. (1966)
WELKER , N. E. , AND L. L. CAMPBELL. 1967 . Unrelatedness of Bacillus amyloliquefaciens and Bacillus subtilis . J. Bacteriol. 94 : 1124 - 1130 . (10.1128/JB.94.4.1124-1130.1967) / J. Bacteriol. / Unrelatedness of Bacillus amyloliquefaciens and Bacillus subtilis by WELKER N. E. (1967)
YOUNG , F. E. 1967 . Competence in Bacillus subtilis transformation system . Nature 213 : 773 - 775 . (10.1038/213773a0) / Nature / Competence in Bacillus subtilis transformation system by YOUNG F. E. (1967)
YOUNG , F. E. , AND J. SPIZIZEN. 1961 . Physiological and genetic factors affecting transformation of Bacillus subtilis . J. Bacteriol. 81 : 823 - 829 . (10.1128/JB.81.5.823-829.1961) / J. Bacteriol. / Physiological and genetic factors affecting transformation of Bacillus subtilis by YOUNG F. E. (1961)
YOUNG , F. E. , AND J. SPIZIZEN. 1963 . Incorporation of deoxyribonucleic acid in the Bacillus subtilis transformation system . J. Bacteriol. 86 : 392 - 400 . (10.1128/JB.86.3.392-400.1963) / J. Bacteriol. / Incorporation of deoxyribonucleic acid in the Bacillus subtilis transformation system by YOUNG F. E. (1963)
YOUNG , F. E. , J. SPIZIZEN , A ND I . CRAWFORD. 1963 . Biochemical aspects of competence in the Bacillus subtilis transformation system. I. Chemical composition of cell walls . J. Biol. Chem. 238 : 3119 - 3125 . (10.1016/S0021-9258(18)51877-9) / J. Biol. Chem. / Biochemical aspects of competence in the Bacillus subtilis transformation system. I. Chemical composition of cell walls by YOUNG F. E. (1963)

Dates

Type	When
Created	5 years, 8 months ago (Jan. 4, 2020, 9:07 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 12:46 p.m.)
Indexed	4 weeks ago (Aug. 7, 2025, 4:46 p.m.)
Issued	57 years, 5 months ago (April 1, 1968)
Published	57 years, 5 months ago (April 1, 1968)
Published Print	57 years, 5 months ago (April 1, 1968)

Funders 0

None

BibTeX

@article{Wilson_1968, title={Nutritional Factors Influencing the Development of Competence in the Bacillus subtilis Transformation System}, volume={95}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.95.4.1439-1449.1968}, DOI={10.1128/jb.95.4.1439-1449.1968}, number={4}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Wilson, Gary A. and Bott, Kenneth F.}, year={1968}, month=apr, pages={1439–1449} }

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