Aromatic Amino Acid Biosynthesis: Gene-Enzyme Relationships in Bacillus subtilis
10.1128/jb.94.5.1706-1714.1967
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Single step mutants of Bacillus subtilis which required either one or all of the aromatic amino acids for growth were isolated. The relevant gene defect was determined for each mutant by enzyme assays in vitro. A mutant deficient in each enzyme step of aromatic amino acid biosynthesis was found with the exceptions of the shikimate kinase and the phenylalanine and tyrosine transaminases. Representative mutants carrying the defective genes were mapped by deoxyribonucleic acid mediated transformation by reference to the aromatic amino acid gene ( aro ) cluster and, alternately, to any of the other unlinked aro genes. The genes coding for dehydroquinate synthetase, 3-enol pyruvylshikimate 5-phosphate synthetase, one form of chorismate mutase, and prephenate dehydrogenase are linked to the aro cluster. Except for the previously identified linkage between the genes of 3-deoxy- d -arabino heptulosonic acid 7-phosphate synthetase and one species of chorismate mutase, the other genes involved in this pathway are neither linked to the aro cluster nor to each other.

Bibliography

Nasser, D., & Nester, E. W. (1967). Aromatic Amino Acid Biosynthesis: Gene-Enzyme Relationships in Bacillus subtilis. Journal of Bacteriology, 94(5), 1706–1714.

Authors 2
  1. DeLill Nasser (first)
  2. E. W. Nester (additional)
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Dates
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Created 5 years, 8 months ago (Jan. 4, 2020, 9:04 p.m.)
Deposited 4 years, 2 months ago (June 12, 2021, 6:21 a.m.)
Indexed 1 year, 10 months ago (Nov. 2, 2023, 9:05 a.m.)
Issued 57 years, 10 months ago (Nov. 1, 1967)
Published 57 years, 10 months ago (Nov. 1, 1967)
Published Print 57 years, 10 months ago (Nov. 1, 1967)
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@article{Nasser_1967, title={Aromatic Amino Acid Biosynthesis: Gene-Enzyme Relationships in Bacillus subtilis}, volume={94}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.94.5.1706-1714.1967}, DOI={10.1128/jb.94.5.1706-1714.1967}, number={5}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Nasser, DeLill and Nester, E. W.}, year={1967}, month=nov, pages={1706–1714} }