DOI: 10.1128/jb.94.4.949-957.1967. Regulation of Glutamine Synthetase X. Effect of Growth Conditions on the Susceptibility of <i>Escherichia coli</i> Glutamine Synthetase to Feedback Inhibition

Regulation of Glutamine Synthetase X. Effect of Growth Conditions on the Susceptibility of Escherichia coli Glutamine Synthetase to Feedback Inhibition

10.1128/jb.94.4.949-957.1967

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The kinetic properties of Escherichia coli glutamine synthetase are markedly influenced by the manner in which the organism is grown. Enzyme obtained from stationary-phase cells grown on glycerol and glutamate is strongely inhibited by each of the eight feedback effectors known to influence this enzyme; however, the enzyme from log-phase cells grown on glucose and growth-limiting concentrations of NH 4 Cl is stimulated by some of these effectors. Of the growth variables examined, nitrogen source and time of harvest were the most important; carbon source and aeration seemed to have no effect. Two purified enzyme preparations have been obtained from cells grown under two different conditions, designated enzymes I and II for convenience. Enzyme I is stimulated by adenosine 5′-monophosphate, histidine, and tryptophan in the transfer assay, whereas enzyme II is strongly inhibited by all effectors tested. Enzyme I has a higher specific activity in the forward assay in the presence of Mg ++ or Co ++ , whereas enzyme II is more active in the presence of Mn ++ .

Bibliography

Kingdon, H. S., & Stadtman, E. R. (1967). Regulation of Glutamine Synthetase X. Effect of Growth Conditions on the Susceptibility of Escherichia coli Glutamine Synthetase to Feedback Inhibition. Journal of Bacteriology, 94(4), 949â957.

Authors 2

Henry S. Kingdon (first)
E. R. Stadtman (additional)

References 9 Referenced 58

DAVIS , B. J. 1964 . Disc electrophoresis-IT. Method and application to human serum proteins . Ann. N. Y. Acad. Sci. 121 : 404 - 427 . (10.1111/j.1749-6632.1964.tb14213.x) / Ann. N. Y. Acad. Sci. / Disc electrophoresis-IT. Method and application to human serum proteins by DAVIS B. J. (1964)
GORNALL , A. G. , C. J. BARDAWILL , A ND M . M. DAVID. 1949 . Determination of serum proteins by means of the biuret reaction . J. Biol. Chem. 177 : 751 - 766 . (10.1016/S0021-9258(18)57021-6) / J. Biol. Chem. / Determination of serum proteins by means of the biuret reaction by GORNALL A. G. (1949)
KINGDON , H. S. , AND E. R. STADTMAN , 1967 . Two E. coli glutamine synthetases with different sensitivities to feedback effectors . Biochem. Biophys. Res. Commun. 27 : 470 - 473 . (10.1016/S0006-291X(67)80008-1) / Biochem. Biophys. Res. Commun. / Two E. coli glutamine synthetases with different sensitivities to feedback effectors by KINGDON H. S. (1967)
LEVIN r OW, L . 1954 . The glutamyltransferase activity of normal and neoplastic tissues . J. Natl. Cancer Inst. 15 : 347 - 352 . / J. Natl. Cancer Inst. / The glutamyltransferase activity of normal and neoplastic tissues by LEVIN OW, L (1954)
MECKE D. K. WULFF AND H. HOLZER. 1966. 957
STADTMAN , E. R. , G. N. COHEN , G. LEBRAS , A ND H . DE ROBICHON-SZULMAJSTER . 1961 . Feed-back inhibition and repression of aspartokinase activity in Escherichia coli and Saccharomyces cerevisiae . J. Biol. Chem. 236 : 2033 - 2038 . (10.1016/S0021-9258(18)64125-0) / J. Biol. Chem. / Feed-back inhibition and repression of aspartokinase activity in Escherichia coli and Saccharomyces cerevisiae by STADTMAN E. R. (1961)
WOOLFOLK , C. A. , B. SHAPIRO , A ND E . R. STADTMAN. 1966 . Regulation of glutamine synthetase. I. Purification and properties of glutamine synthetase from Escherichia coli . Arch. Biochem. Biophys. 116 : 177 - 192 . / Arch. Biochem. Biophys. / Regulation of glutamine synthetase. I. Purification and properties of glutamine synthetase from Escherichia coli by WOOLFOLK C. A. (1966)
WOOLFOLK , C. A. , AND E. R. STADTMAN. 1964 . Cumulative feedback inhibition in the multiple end product regulation of glutamine synthetase activity in Escherichia coli . Biochem. Biophys. Res. Commun. 17 : 313 - 319 . (10.1016/0006-291X(64)90003-8) / Biochem. Biophys. Res. Commun. / Cumulative feedback inhibition in the multiple end product regulation of glutamine synthetase activity in Escherichia coli by WOOLFOLK C. A. (1964)
WOOLFOLK , C. A. , AND E. R. STADTMAN. 1967 . Regulation of glutamine synthetase. III. Cumulative feedback inhibition of glutamine synthetase from Escherichia coli . Arch. Biochem. Biophys. 118 : 736 - 755 . / Arch. Biochem. Biophys. / Regulation of glutamine synthetase. III. Cumulative feedback inhibition of glutamine synthetase from Escherichia coli by WOOLFOLK C. A. (1967)

Dates

Type	When
Created	5 years, 7 months ago (Jan. 4, 2020, 9:02 p.m.)
Deposited	4 years, 2 months ago (June 12, 2021, 6:55 a.m.)
Indexed	2 days, 5 hours ago (Aug. 30, 2025, 12:28 p.m.)
Issued	57 years, 11 months ago (Oct. 1, 1967)
Published	57 years, 11 months ago (Oct. 1, 1967)
Published Print	57 years, 11 months ago (Oct. 1, 1967)

Funders 0

None

BibTeX

@article{Kingdon_1967, title={Regulation of Glutamine Synthetase X. Effect of Growth Conditions on the Susceptibility of Escherichia coli Glutamine Synthetase to Feedback Inhibition}, volume={94}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.94.4.949-957.1967}, DOI={10.1128/jb.94.4.949-957.1967}, number={4}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Kingdon, Henry S. and Stadtman, E. R.}, year={1967}, month=oct, pages={949–957} }

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