Fine Structure of the Cytomembranes of Nitrosocystis oceanus
10.1128/jb.94.2.422-433.1967
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Thin-sectioned, negatively stained, and freeze-etched preparations of Nitrosocystis oceanus cytomembranes were compared. The cytomembranes in freeze-etched cells were covered with 80- to 120-A particles. When cells were disrupted and differentially centrifuged, various membrane and particle fractions were obtained. Negatively stained membrane fragments from the pellet centrifuged at 3,000 × g showed 70- to 80-A stalked particles, whereas those from the pellet centrifuged at 39,000 × g exhibited a crystalline array of subunits with a 30- to 40-A periodicity. High-speed supernatant and pellet fractions centrifuged at greater than 39,000 × g contained 40- to 120-A free particles but no membranes. In chemically fixed cells, 40-A particles were found embedded in the matrix of membranes. Results suggest that the larger 80- to 120-A particles are enzyme complexes, whereas the smaller 30- to 40-A particles represent a structural protein or a lipoprotein of the membrane.

Bibliography

Remsen, C. C., Valois, F. W., & Watson, S. W. (1967). Fine Structure of the Cytomembranes of Nitrosocystis oceanus. Journal of Bacteriology, 94(2), 422–433.

Authors 3
  1. Charles C. Remsen (first)
  2. Frederica W. Valois (additional)
  3. Stanley W. Watson (additional)
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Dates
Type When
Created 5 years, 7 months ago (Jan. 4, 2020, 9:01 p.m.)
Deposited 3 years, 11 months ago (Sept. 3, 2021, 10:09 p.m.)
Indexed 1 year, 7 months ago (Jan. 11, 2024, 5:56 a.m.)
Issued 58 years ago (Aug. 1, 1967)
Published 58 years ago (Aug. 1, 1967)
Published Print 58 years ago (Aug. 1, 1967)
Funders 0

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@article{Remsen_1967, title={Fine Structure of the Cytomembranes of Nitrosocystis oceanus}, volume={94}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.94.2.422-433.1967}, DOI={10.1128/jb.94.2.422-433.1967}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Remsen, Charles C. and Valois, Frederica W. and Watson, Stanley W.}, year={1967}, month=aug, pages={422–433} }