DOI: 10.1128/jb.93.1.427-437.1967. Production and Ultrastructure of Lysozyme and Ethylenediaminetetraacetate-Lysozyme Spheroplasts of <i>Escherichia coli</i>

Production and Ultrastructure of Lysozyme and Ethylenediaminetetraacetate-Lysozyme Spheroplasts of Escherichia coli

10.1128/jb.93.1.427-437.1967

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Spheroplast production by lysozyme and ethylenediaminetetraacetate (EDTA) was examined as a means of obtaining osmotically sensitive cells for studies of enzyme localization. Physiologically young cells plasmolyzed with 0.5 m sucrose in 0.01 m tris(hydroxymethyl)aminomethane (Tris) buffer ( p H 7, 8, or 9) were quantitatively converted to plasmolyzed osmotically sensitive rods after lysozyme treatment. Although such cells were osmotically sensitive, a 1:1 dilution in Tris buffer was necessary for conversion of rods into spheroplasts. Addition of EDTA resulted in a rapid conversion of the plasmolyzed spheroplasts into spherical structures devoid of a plasmolysis vacuole. These structures, which we call EDTA-lysozyme spheroplasts, contained a number of attached membranes. We believe that this conversion results from a weakening of the outer trilaminar component of the cell wall by EDTA, resulting in the collapse of the plasmolysis vacuole. Dilution of sucrose below 0.15 m also resulted in the collapse of the plasmolysis vacuole. Both the lysozyme spheroplasts and the EDTA-lysozyme spheroplasts were osmotically sensitive. Thin sections of the EDTA-lysozyme spheroplasts demonstrated that the outer trilaminar component of the cell wall was broken, exposing large areas of the cytoplasmic membrane to the environment.

Bibliography

Birdsell, D. C., & Cota-Robles, E. H. (1967). Production and Ultrastructure of Lysozyme and Ethylenediaminetetraacetate-Lysozyme Spheroplasts of Escherichia coli. Journal of Bacteriology, 93(1), 427â437.

Authors 2

D. C. Birdsell (first)
E. H. Cota-Robles (additional)

References 28 Referenced 239

ADAMs M. H. 1959. Bacteriophages. Interscience Publishers Inc. New York.
ASBELL , M. A. , AND R. G. EAGON. 1966 . The role of multivalent cations in the organization and structure of bacterial cell walls . Biochem. Biophys. Res. Commun. 22 : 664 - 671 . (10.1016/0006-291X(66)90198-7) / Biochem. Biophys. Res. Commun. / The role of multivalent cations in the organization and structure of bacterial cell walls by ASBELL M. A. (1966)
BRENNER , S., F. A. DARK , P. GERHARDT , M. H. JEYNES , 0. KAN DLER , E. KELLENBERGER , E. KLINEBERGER-NOBEL , K. MCQUILLEN , M. RUBIO-HUERTOS , M. R. J. SALTON , R. E. STRANGE , J. ToMcs IK , A ND C . WEIBULL. 1958 . Bacterial protoplasts . Nature 181 : 1713 - 1714 . (10.1038/1811713b0) / Nature / Bacterial protoplasts by BRENNER S., F. (1958)
CARSON , K. J. , AND R. G. EAGON. 1966 . Lysozyme sensitivity of the cell wall of Pseudomonas aeruginosa: further evidence for the role of the nonpeptidoglycan components in cell wall rigidity . Can. J. Microbiol. 12 : 105 - 108 . (10.1139/m66-015) / Can. J. Microbiol. / Lysozyme sensitivity of the cell wall of Pseudomonas aeruginosa: further evidence for the role of the nonpeptidoglycan components in cell wall rigidity by CARSON K. J. (1966)
CORDONNIER . C. , AND G. BERNARDI. 1965 . Localization of E. coli endonuclease I . Biochem. Biophys. Res. Commun. 20 : 555 - 559 . (10.1016/0006-291X(65)90434-1) / Biochem. Biophys. Res. Commun. / Localization of E. coli endonuclease I by CORDONNIER C. (1965)
COTA-ROBLES , E. H. , AND P. H. DUNCAN. 1962 . The effect of D-glutamic acid upon spheroplast formation in Escherichia coli B . Exptl. Cell Res. 28 : 342 - 349 . (10.1016/0014-4827(62)90288-4) / Exptl. Cell Res. / The effect of D-glutamic acid upon spheroplast formation in Escherichia coli B by COTA-ROBLES E. H. (1962)
DRESDEN , M. , AND M. B. HOAGLAND. 1965 . Polyribosomes from Escherichia coli: enzymatic method for isolation . Science 149 : 647 - 649 . (10.1126/science.149.3684.647) / Science / Polyribosomes from Escherichia coli: enzymatic method for isolation by DRESDEN M. (1965)
FRASER , D. , AND E. A. JERREL. 1953 . The amino acid composition of T3 bacteriophage . J. Biol. Chem. 205 : 291 - 295 . (10.1016/S0021-9258(19)77254-8) / J. Biol. Chem. / The amino acid composition of T3 bacteriophage by FRASER D. (1953)
GUTHRIE , G. D. , AND R. L. SINSHEIMER. 1963 . Observations on the infection of bacterial protoplasts with the deoxyribonucleic acid of bacteriophage ox 174 . Biochim. Biophys. Acta 72 : 290 - 297 . (10.1016/0926-6550(63)90342-6) / Biochim. Biophys. Acta / Observations on the infection of bacterial protoplasts with the deoxyribonucleic acid of bacteriophage ox 174 by GUTHRIE G. D. (1963)
HOFSCHNEIDER , P. H. 1960 . Zur Wandstructur von Escherichia coli B Sphaeroplasten . Proc. European Regional Conf. Electron Microscopy Delft , vol. 2 , p. 1028 - 1032 . / Proc. European Regional Conf. Electron Microscopy Delft / Zur Wandstructur von Escherichia coli B Sphaeroplasten by HOFSCHNEIDER P. H. (1960)
. KELLENBERGER , E. , AND A. RYTER. 1958 . Cell wall and cytoplasmic membrane of Escherichia coli . J. Biochem. Biophys. Cytol. 4 : 323 - 326 . (10.1083/jcb.4.3.323) / J. Biochem. Biophys. Cytol. / Cell wall and cytoplasmic membrane of Escherichia coli by KELLENBERGER E. (1958)
LEDERBERG , J. , AND J. ST. CLAIR . 1958 . Protoplasts and L-type growth of Escherichia coli . J. Bacteriol. 75 : 143 - 150 . (10.1128/jb.75.2.143-160.1958) / J. Bacteriol. / Protoplasts and L-type growth of Escherichia coli by LEDERBERG J. (1958)
LEIVE , L. 1965 . Release of lipopolysaccharide by EDTA treatment of E. coli . Biochem. Biophys. Res. Commun. 21 : 290 - 296 . (10.1016/0006-291X(65)90191-9) / Biochem. Biophys. Res. Commun. / Release of lipopolysaccharide by EDTA treatment of E. coli by LEIVE L. (1965)
MCQUILLEN K. 1960. Bacterial protoplasts p. 249-359. In I. C. Gunsalus and R. Y. Stanier [ed.] The bacteria vol. 1. Academic Press Inc. New York.
MALAMY , M. H. , AND B. L. HORECKER. 1964 . Release of alkaline phosphatase from cells of Escherichia coli upon Iysozyme spheroplast formation . Biochemistry 3 : 1889 - 1893 . (10.1021/bi00900a017) / Biochemistry / Release of alkaline phosphatase from cells of Escherichia coli upon Iysozyme spheroplast formation by MALAMY M. H. (1964)
MALAMY , M. H. , AND B. L. HORECKER. 1964 . Purification and crystallization of the alkaline phosphatase of Escherichia coli . Biochemistry 3 : 1893 - 1897 . (10.1021/bi00900a018) / Biochemistry / Purification and crystallization of the alkaline phosphatase of Escherichia coli by MALAMY M. H. (1964)
MEYER , F., R. P. MACKAL , M. TAO , A ND E . A. EVANS , JR. 1961 . Infectious deoxyribonucleic acid from bacteriophage . J. Biol. Chem. 236 : 1141 - 1143 . (10.1016/S0021-9258(18)64256-5) / J. Biol. Chem. / Infectious deoxyribonucleic acid from bacteriophage by MEYER F., R. (1961)
Mu RRAY , R. G. E., P. STEED , A ND H . E. ELSON. 1965 . The location of the mucopeptide in sections of cell wall of Escherichia coli and other gram-negative bacteria . Can. J. Microbiol. 11 : 547 - 560 . (10.1139/m65-072) / Can. J. Microbiol. / The location of the mucopeptide in sections of cell wall of Escherichia coli and other gram-negative bacteria by RRAY R. G. (1965)
NEU , H. C. , AND L. A. HEPPEL. 1964 . On the surface location of enzymes in E. coli . Biochem. Biophys. Res. Commun. 17 : 215 - 219 . (10.1016/0006-291X(64)90386-9) / Biochem. Biophys. Res. Commun. / On the surface location of enzymes in E. coli by NEU H. C. (1964)
NEU , H. C. , AND L. A. HEPPEL. 1964 . The release of ribonuclease into the medium when Escherichia coli cells are converted to spheroplasts . J. Biol. Chem. 239 : 3893 - 3900 . (10.1016/S0021-9258(18)91220-2) / J. Biol. Chem. / The release of ribonuclease into the medium when Escherichia coli cells are converted to spheroplasts by NEU H. C. (1964)
NEU , H. C. , AND L. A. HEPPEL. 1965 . The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts . J. Biol. Chem. 240 : 3685 - 3692 . (10.1016/S0021-9258(18)97200-5) / J. Biol. Chem. / The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts by NEU H. C. (1965)
NOLLER , E. C. , AND S. E. HARTSELL. 1961 . Bacteriolysis of Enterobacteriaceae. I. Lysis by four lytic systems utilizing Iysozyme . J. Bacteriol. 81 : 482 - 491 . (10.1128/jb.81.3.482-491.1961) / J. Bacteriol. / Bacteriolysis of Enterobacteriaceae. I. Lysis by four lytic systems utilizing Iysozyme by NOLLER E. C. (1961)
PROTASS , J. J. , AND D. KORN. 1966 . Impairment of temperate bacteriophage adsorption by brief treatment of Escherichia coli with dilute solutions of ethylenediaminetetraacetate . J. Bacteriol. 91 : 143 - 147 . (10.1128/jb.91.1.143-147.1966) / J. Bacteriol. / Impairment of temperate bacteriophage adsorption by brief treatment of Escherichia coli with dilute solutions of ethylenediaminetetraacetate by PROTASS J. J. (1966)
RYTER , A. , AND F. JACOB. 1966 . E1tude morphologique de la liaison du noyau 'a la membrane chez E. coli et chez les protoplasts de B. subtilis . Ann. Inst. Pasteur 110 : 801 - 812 . / Ann. Inst. Pasteur / E1tude morphologique de la liaison du noyau 'a la membrane chez E. coli et chez les protoplasts de B. subtilis by RYTER A. (1966)
Sis TR o M, W . R. 1958 . On the physical state of intracellularly accumulated substrates of jS-galactoside-permease in Escherichia coli . Biochim. Biophys. Acta 29 : 579 - 587 . (10.1016/0006-3002(58)90015-5) / Biochim. Biophys. Acta / On the physical state of intracellularly accumulated substrates of jS-galactoside-permease in Escherichia coli by TR M, W (1958)
VENABLE , J. H. , AND R. COGGESHALL. 1965 . A simplified lead citrate strain for use in electron microscopy . J. Cell Biol. 25 : 407 - 408 . (10.1083/jcb.25.2.407) / J. Cell Biol. / A simplified lead citrate strain for use in electron microscopy by VENABLE J. H. (1965)
Voss, J. C. 1964 . Lysozyme Iysis of gram-negative bacteria without production of spheroplasts . J. Gen. Microbiol. 35 : 313 - 317 . (10.1099/00221287-35-2-313) / J. Gen. Microbiol. / Lysozyme Iysis of gram-negative bacteria without production of spheroplasts by C. (1964)
WEIDEL W. G. KOCH AND F. LOHss. 1964. Uber die Zellmembran von Escherichia coli B. II. Der Rezeptokomplex fur die Bakteriophagen T3 T4 und T7 Vergleichende Chemische-analytische Untersuchungen. Z. Naturforsch. 9b: 398-406. (10.1515/znb-1954-0604)

Dates

Type	When
Created	5 years, 7 months ago (Jan. 4, 2020, 8:57 p.m.)
Deposited	3 years, 11 months ago (Sept. 3, 2021, 9:55 p.m.)
Indexed	2 months, 2 weeks ago (June 12, 2025, 12:28 p.m.)
Issued	58 years, 8 months ago (Jan. 1, 1967)
Published	58 years, 8 months ago (Jan. 1, 1967)
Published Print	58 years, 8 months ago (Jan. 1, 1967)

Funders 0

None

BibTeX

@article{Birdsell_1967, title={Production and Ultrastructure of Lysozyme and Ethylenediaminetetraacetate-Lysozyme Spheroplasts of Escherichia coli}, volume={93}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.93.1.427-437.1967}, DOI={10.1128/jb.93.1.427-437.1967}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Birdsell, D. C. and Cota-Robles, E. H.}, year={1967}, month=jan, pages={427–437} }

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      "year": "1966",
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      "journal-title": "J. Bacteriol."
    },
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      "year": "1966",
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      "volume": "29",
      "author": "TR M, W",
      "year": "1958",
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    },
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      "author": "VENABLE J. H.",
      "year": "1965",
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