Electron Microscopy of the Cell Envelope of Ferrobacillus ferrooxidans Prepared by Freeze-Etching and Chemical Fixation Techniques
10.1128/jb.92.6.1765-1771.1966
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Remsen , C. C. (Swiss Federation Institute of Technology, Zurich, Switzerland), and D. G. Lundgren . Electron microscopy of the cell envelope of Ferrobacillus ferrooxidans prepared by freeze-etching and chemical fixation techniques. J. Bacteriol. 92: 1765–1771. 1966.—A comparison was made of the fine structure of the cell envelope of the gram-negative bacterium Ferrobacillus ferrooxidans when cells were prepared for microscopy by freeze-etching and chemical fixation techniques. Cell envelopes of chemically fixed cells appeared as five separate layers distinguishable by their location and electron density. Frozen-etched cells showed a three-layered complex with each layer measuring approximately 100 A in thickness. The latter technique is considered to be “artifact-free” and, as a technique, yields purely morphological information on the natural state. The three layers revealed by freeze-etching are: the outer layer, a lipoprotein-lipopolysaccharide layer; the middle layer, a layer composed of globular protein attached to fibrillar mucopeptide; and the innermost layer, the cytoplasmic membrane. The latter was covered with 100 to 120 A particles. The relationship of the aforementioned layers to those seen in chemically fixed cells is discussed.

Bibliography

Remsen, C., & Lundgren, D. G. (1966). Electron Microscopy of the Cell Envelope of Ferrobacillus ferrooxidans Prepared by Freeze-Etching and Chemical Fixation Techniques. Journal of Bacteriology, 92(6), 1765–1771.

Authors 2
  1. Charles Remsen (first)
  2. D. G. Lundgren (additional)
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Dates
Type When
Created 5 years, 7 months ago (Jan. 4, 2020, 8:57 p.m.)
Deposited 4 years ago (July 29, 2021, 1:22 p.m.)
Indexed 1 year, 9 months ago (Nov. 12, 2023, 5:15 a.m.)
Issued 58 years, 8 months ago (Dec. 1, 1966)
Published 58 years, 8 months ago (Dec. 1, 1966)
Published Print 58 years, 8 months ago (Dec. 1, 1966)
Funders 0

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@article{Remsen_1966, title={Electron Microscopy of the Cell Envelope of Ferrobacillus ferrooxidans Prepared by Freeze-Etching and Chemical Fixation Techniques}, volume={92}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.92.6.1765-1771.1966}, DOI={10.1128/jb.92.6.1765-1771.1966}, number={6}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Remsen, Charles and Lundgren, D. G.}, year={1966}, month=dec, pages={1765–1771} }