DOI: 10.1128/jb.92.1.186-196.1966. Thymidylate Synthesis and Aminopterin Resistance in <i>Bacillus subtilis</i>

Thymidylate Synthesis and Aminopterin Resistance in Bacillus subtilis

10.1128/jb.92.1.186-196.1966

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Wilson, Melba Carr (Brown University, Providence, R.I.), James L. Farmer, and Frank Rothman . Thymidylate synthesis and aminopterin resistance in Bacillus subtilis . J. Bacteriol. 92: 186–196. 1966.—The thymine-requirement of Bacillus subtilis 168 thy results from mutation in two unlinked genes (i.e., genetic loci) designated thyA and thyB . The thyB gene is located between the met and ile markers. Both thyA + thyB and thyA thyB + strains are phenotypically thy + . ThyA + thyB strains resemble the wild type in their sensitivity to aminopterin, poor incorporation of exogenous thymine into deoxyribonucleic acid (DNA), and high level of thymidylate synthetase activity in crude extracts. ThyA thyB + strains are resistant to aminopterin in the presence of thymine, incorporate exogenous thymine into DNA, and have no detectable thymidylate synthetase activity. Experiments designed to elucidate the role of the thyB gene indicate that it specifies an alternate pathway of thymidylate synthesis, similar to thymidylate synthetase but requiring a cofactor other than tetrahydrofolate. The mechanism of selection of thymine-requiring mutants by aminopterin is revealed by these results.

Bibliography

Wilson, M. C., Farmer, J. L., & Rothman, F. (1966). Thymidylate Synthesis and Aminopterin Resistance in Bacillus subtilis. Journal of Bacteriology, 92(1), 186â196.

Authors 3

Melba Carr Wilson (first)
James L. Farmer (additional)
Frank Rothman (additional)

References 33 Referenced 39

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Dates

Type	When
Created	5 years, 7 months ago (Jan. 4, 2020, 8:54 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:21 p.m.)
Indexed	1 year, 5 months ago (March 29, 2024, 9:47 a.m.)
Issued	59 years, 2 months ago (July 1, 1966)
Published	59 years, 2 months ago (July 1, 1966)
Published Print	59 years, 2 months ago (July 1, 1966)

Funders 0

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BibTeX

@article{Wilson_1966, title={Thymidylate Synthesis and Aminopterin Resistance in Bacillus subtilis}, volume={92}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.92.1.186-196.1966}, DOI={10.1128/jb.92.1.186-196.1966}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Wilson, Melba Carr and Farmer, James L. and Rothman, Frank}, year={1966}, month=jul, pages={186–196} }

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