DOI: 10.1128/jb.84.4.615-624.1962. GENOTYPIC ALTERATIONS ASSOCIATED WITH AVIRULENCE IN STREPTOMYCIN-RESISTANT<i>PASTEURELLA PESTIS</i>

GENOTYPIC ALTERATIONS ASSOCIATED WITH AVIRULENCE IN STREPTOMYCIN-RESISTANTPASTEURELLA PESTIS

10.1128/jb.84.4.615-624.1962

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Brubaker, RobertR. (Fort Detrick, Frederick, Md.)and Michael J. Surgalla. Genotypic alterations associated with avirulence in streptomycin-resistantPasteurella pestis. J. Bacteriol.84:615–624. 1962.—Avirulence inPasteurella pestis, as indicated by the existence of a new genotype, was associated with the genetic loss of Ca++dependence rather than with the usual concomitant loss of ability to produce virulence antigens. Strains of the new type exhibited all four of the known virulence factors ofP. pestisbut were avirulent for mice and guinea pigs (ld50> 108cells). In addition to producing virulence antigens, the new strains formed rough colonies at 37 C when grown on Ca++-deficient medium, as opposed to typical avirulent strains which formed smooth colonies on this medium and failed to produce virulence antigens. Growth of the new strains in a corresponding Ca++-deficient broth medium was slow, as compared with that of typical avirulent strains, but was more rapid than that of virulent strains. The new strains were obtained (i) at 26 C by selecting for resistance to streptomycin or (ii) at 37 C by isolation from rough colonies growing on a Ca++-deficient agar medium plated with either a virulent streptomycin-sensitive or -resistant culture. Usually, isolates obtained at 26 C exhibited a marked reduction in ability to form colonies upon primary plating at 37 C. Of 108 streptomycin-resistant isolates obtained from six virulent strains, 20% were of the new type. In addition, five isolates were of reduced virulence (mouseld50∼ 104cells) but differed in being Ca++-dependent; they possessed all of the known virulence properties. Certain streptomycin-resistant strains failed to give rise to avirulent colonies when plated on a Ca++-deficient agar medium containing streptomycin. The significance of this phenomenon, which was not exhibited by the five strains of reduced virulence, is discussed, as are some apparent differences between isolates from a glycerol-fermenting strain and similar isolates obtained from five nonglycerol-fermenting strains.

Bibliography

Brubaker, R. R., & Surgalla, M. J. (1962). GENOTYPIC ALTERATIONS ASSOCIATED WITH AVIRULENCE IN STREPTOMYCIN-RESISTANTPASTEURELLA PESTIS. Journal of Bacteriology, 84(4), 615â624.

Authors 2

Robert R. Brubaker (first)
Michael J. Surgalla (additional)

References 20 Referenced 11

BAKER , E. E. , H. SOMMER , L. E. FOSTER , E. MEYER , A ND K . F. MEYER. 1952 . Studies on immunization against plague. I. The isolation and characterization of the soluble antigen of Pasteurella pestis . J. Immunol. 68 : 131 - 145 . / J. Immunol. / Studies on immunization against plague. I. The isolation and characterization of the soluble antigen of Pasteurella pestis by BAKER E. E. (1952)
BRUBAKER R. R. AND M. J. SURGALLA. 1961a. Studies on the Ca++ requirement of virulent Pasteurella pestis. Bacteriol. Proc. p. 98.
BRUBAKER , R. R. , AND M. J. SURGALLA. 1961 b. Pesticins. I. Pesticin-bacterium interrelationships, and environmental factors influencing activity . J. Bacteriol. 82 : 940 - 949 . (10.1128/jb.82.6.940-949.1961) / J. Bacteriol. / Pesticins. I. Pesticin-bacterium interrelationships, and environmental factors influencing activity by BRUBAKER R. R. (1961)
BURROWS T. W. 1955. The basis of virulence for mice of Pasteurella pestis p. 151-175. In Mechanisms of microbial pathogenicity. Cambridge University Press Cambridge.
BURROWS , T. W. 1957 . Virulence of Pasteurella pestis . Nature 179 : 1246 - 1247 . (10.1038/1791246a0) / Nature / Virulence of Pasteurella pestis by BURROWS T. W. (1957)
BURROWS , T. W. 1960 . Biochemical properties of virulent and avirulent strains of bacteria: Salmonella typhosa and Pasteurella pestis . Ann. N. Y. Acad. Sci. 88 : 1125 - 1135 . (10.1111/j.1749-6632.1960.tb20102.x) / Ann. N. Y. Acad. Sci. / Biochemical properties of virulent and avirulent strains of bacteria: Salmonella typhosa and Pasteurella pestis by BURROWS T. W. (1960)
BURROWS , T. W. , AND G. A. BACON. 1956 . The basis of virulence in Pasteurella pestis: an antigen determining virulence . Brit. J. Exptl. Pathol. 37 : 481 - 493 . / Brit. J. Exptl. Pathol. / The basis of virulence in Pasteurella pestis: an antigen determining virulence by BURROWS T. W. (1956)
BURROWS , T. W. , AND G. A. BACON. 1958 . The effects of loss of different virulence determinants on the virulence and immunogenicity of strains of Pasteurella pestis . Brit. J. Exptl. Pathol. 39 : 278 - 291 . / Brit. J. Exptl. Pathol. / The effects of loss of different virulence determinants on the virulence and immunogenicity of strains of Pasteurella pestis by BURROWS T. W. (1958)
DONAVAN , J. E. , D. HAM , G. M. FUKUI , A ND M . J. SURGALLA. 1961 . Role of the capsule of Pasteurella pestis in bubonic plague in the guinea pig . J. Infectious Diseases 109 : 154 - 157 . (10.1093/infdis/109.2.154) / J. Infectious Diseases / Role of the capsule of Pasteurella pestis in bubonic plague in the guinea pig by DONAVAN J. E. (1961)
EISLER , D. M. , G. KUBIK , A ND H . PRESTON. 1958 . Colonial morphology and virulence of Pasteurella pestis . J. Bacteriol. 76 : 41 - 47 . (10.1128/jb.76.1.41-47.1958) / J. Bacteriol. / Colonial morphology and virulence of Pasteurella pestis by EISLER D. M. (1958)
GARBER , E. E. , K. NOBLE , A ND N . CAROUSO. 1953 . Genetic studies on the development of streptomycin resistance in Pasteurella pestis . J. Bacteriol. 65 : 485 - 489 . (10.1128/jb.65.5.485-489.1953) / J. Bacteriol. / Genetic studies on the development of streptomycin resistance in Pasteurella pestis by GARBER E. E. (1953)
HIGUCHI , K., L. L. KUPFERBERG , A ND J . L. SMITH. 1959 . Studies on the nutrition and physiology of Pasteurella pestis. III. Effects of calcium ions on the growth of virulent and avirulent strains of Pasteurella pestis . J. Bacteriol. 77 : 317 - 321 . (10.1128/jb.77.3.317-321.1959) / J. Bacteriol. / Studies on the nutrition and physiology of Pasteurella pestis. III. Effects of calcium ions on the growth of virulent and avirulent strains of Pasteurella pestis by HIGUCHI K., L. (1959)
HIGUCHI , K. , AND J. L. SMITH. 1961 . Studies on the nutrition and physiology of Pasteurella pestis. VI. A differential plating medium for the estimation of the mutation rate to avirulence . J. Bacteriol. 81 : 605 - 608 . (10.1128/JB.81.4.605-608.1961) / J. Bacteriol. / Studies on the nutrition and physiology of Pasteurella pestis. VI. A differential plating medium for the estimation of the mutation rate to avirulence by HIGUCHI K. (1961)
JACKSON , S. , AND T. W. BURROWS. 1956 a. The pigmentation of Pasteurella pestis on a defined medium containing haemin . Brit. J. Exptl. Pathol. 37 : 570 - 576 . / Brit. J. Exptl. Pathol. / The pigmentation of Pasteurella pestis on a defined medium containing haemin by JACKSON S. (1956)
JACKSON , S. , AND T. W. BURROWS. 1956 b. The virulence-enhancing effect of iron on non-pigmented mutants of virulent strains of Pasteurella pestis . Brit. J. Exptl. Pathol. 37 : 577 - 583 . / Brit. J. Exptl. Pathol. / The virulence-enhancing effect of iron on non-pigmented mutants of virulent strains of Pasteurella pestis by JACKSON S. (1956)
LAWTON , W. D. , G. M. FUKUI , A ND M . J. SURGALLA. 1960 . Studies on the antigens of Pasteurella pestis and Pasteurella pseudotuberculosis . J. Immunol. 84 : 475 - 479 . (10.4049/jimmunol.84.5.475) / J. Immunol. / Studies on the antigens of Pasteurella pestis and Pasteurella pseudotuberculosis by LAWTON W. D. (1960)
MATNEY T. S. E. P. GOLDSCHMIDT AND H. T. BAUSUM. 1960. Genetic analysis of streptomycin dependent mutants of Salmonella typhimurium. Bacteriol. Proc. p. 186.
REED , L. J. , AND H. MUENCH. 1938 . A simple method of estimating fifty per cent endpoints . Am. J. Hyg. 27 : 493 - 497 . / Am. J. Hyg. / A simple method of estimating fifty per cent endpoints by REED L. J. (1938)
SURGALLA , M. J. 1960 . Properties of virulent and avirulent strains of Pasteurella pestis . Ann. N. Y. Acad. Sci. 88 : 1136 - 1145 . (10.1111/j.1749-6632.1960.tb20103.x) / Ann. N. Y. Acad. Sci. / Properties of virulent and avirulent strains of Pasteurella pestis by SURGALLA M. J. (1960)
WESSMAN , G. E. , D. E. MILLER , A ND M . J. SURGALLA. 1958 . Toxic effect of glucose on virulent Pasteurlla pestis in chemically defined media . J. Bacteriol. 76 : 368 - 375 . (10.1128/jb.76.4.368-375.1958) / J. Bacteriol. / Toxic effect of glucose on virulent Pasteurlla pestis in chemically defined media by WESSMAN G. E. (1958)

Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 3:47 p.m.)
Deposited	1 year, 11 months ago (Sept. 24, 2023, 2:14 p.m.)
Indexed	1 year, 9 months ago (Nov. 21, 2023, 12:36 a.m.)
Issued	62 years, 10 months ago (Oct. 1, 1962)
Published	62 years, 10 months ago (Oct. 1, 1962)
Published Print	62 years, 10 months ago (Oct. 1, 1962)

Funders 0

None

BibTeX

@article{Brubaker_1962, title={GENOTYPIC ALTERATIONS ASSOCIATED WITH AVIRULENCE IN STREPTOMYCIN-RESISTANTPASTEURELLA PESTIS}, volume={84}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.84.4.615-624.1962}, DOI={10.1128/jb.84.4.615-624.1962}, number={4}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Brubaker, Robert R. and Surgalla, Michael J.}, year={1962}, month=oct, pages={615–624} }

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