Biosynthesis of the N-Linked Glycan inCampylobacter jejuniand Addition onto Protein through Block Transfer
10.1128/jb.188.7.2427-2434.2006
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

ABSTRACTIn eukaryotes, N-linked protein glycosylation is a universal modification involving addition of preformed oligosaccharides to select Asn-Xaa-Ser/Thr motifs and influencing multiple biological events. We recently demonstrated thatCampylobacter jejuniis the first member of theBacteriato possess an N-linked glycan pathway. In this study, high-resolution magic angle spinning nuclear magnetic resonance (HR-MAS NMR) was applied to probe and quantitateC. jejuni N-glycan biosynthesis in vivo. To confirm HR-MAS NMR findings, glycosylation mutants were screened for chicken colonization potential, and glycoproteins were examined by mass spectrometry and lectin blotting. Consistent with the mechanism in eukaryotes, the combined data indicate that bacterial glycans are assembled en bloc, emphasizing the evolutionary conservation of protein N glycosylation. We also show that under the conditions examined, PglG plays no role in glycan biosynthesis, PglI is the glucosyltransferase and the putative ABC transporter, and WlaB (renamed PglK) is required for glycan assembly. These studies underpin the mechanism of N-linked protein glycosylation inBacteriaand provide a simple model system for investigating protein glycosylation and for exploitation in glycoengineering.

Bibliography

Kelly, J., Jarrell, H., Millar, L., Tessier, L., Fiori, L. M., Lau, P. C., Allan, B., & Szymanski, C. M. (2006). Biosynthesis of the N-Linked Glycan inCampylobacter jejuniand Addition onto Protein through Block Transfer. Journal of Bacteriology, 188(7), 2427–2434.

Authors 8
  1. John Kelly (first)
  2. Harold Jarrell (additional)
  3. Lorna Millar (additional)
  4. Luc Tessier (additional)
  5. Laura M. Fiori (additional)
  6. Peter C. Lau (additional)
  7. Brenda Allan (additional)
  8. Christine M. Szymanski (additional)
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Dates
Type When
Created 19 years, 5 months ago (March 17, 2006, 4:27 a.m.)
Deposited 2 years, 3 months ago (May 6, 2023, 4:03 p.m.)
Indexed 3 months ago (June 6, 2025, 4:08 a.m.)
Issued 19 years, 5 months ago (April 1, 2006)
Published 19 years, 5 months ago (April 1, 2006)
Published Print 19 years, 5 months ago (April 1, 2006)
Funders 0

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@article{Kelly_2006, title={Biosynthesis of the N-Linked Glycan inCampylobacter jejuniand Addition onto Protein through Block Transfer}, volume={188}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.188.7.2427-2434.2006}, DOI={10.1128/jb.188.7.2427-2434.2006}, number={7}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Kelly, John and Jarrell, Harold and Millar, Lorna and Tessier, Luc and Fiori, Laura M. and Lau, Peter C. and Allan, Brenda and Szymanski, Christine M.}, year={2006}, month=apr, pages={2427–2434} }