DOI: 10.1128/jb.188.2.599-608.2006. The β-Oxidation Systems of<i>Escherichia coli</i>and<i>Salmonella enterica</i>Are Not Functionally Equivalent

The β-Oxidation Systems ofEscherichia coliandSalmonella entericaAre Not Functionally Equivalent

10.1128/jb.188.2.599-608.2006

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACTBased on its genome sequence, the pathway of β-oxidative fatty acid degradation inSalmonella entericaserovar Typhimurium LT2 has been thought to be identical to the well-characterizedEscherichia coliK-12 system. We report that wild-type strains ofS. entericagrow on decanoic acid, whereas wild-typeE. colistrains cannot. Mutant strains (carryingfadR) of both organisms in which the genes of fatty acid degradation (fad) are expressed constitutively are readily isolated. TheS. enterica fadRstrains grow more rapidly than the wild-type strains on decanoic acid and also grow well on octanoic and hexanoic acids (which do not support growth of wild-type strains). By contrast,E. coli fadRstrains grow well on decanoic acid but grow only exceedingly slowly on octanoic acid and fail to grow at all on hexanoic acid. The two wild-type organisms also differed in the ability to grow on oleic acid when FadR was overexpressed. Under these superrepression conditions,E. colifailed to grow, whereasS. entericagrew well. Exchange of the wild-typefadRgenes between the two organisms showed this to be a property ofS. entericarather than of the FadR proteins per se. This difference in growth was attributed toS. entericahaving higher cytosolic levels of the inducing ligands, long-chain acyl coenzyme As (acyl-CoAs). The most striking results were the differences in the compositions of CoA metabolites of strains grown with octanoic acid or oleic acid.S. entericacleanly converted all of the acid to acetyl-CoA, whereasE. coliaccumulated high levels of intermediate-chain-length products. Exchange of homologous genes between the two organisms showed that theS. entericaFadE and FadBA enzymes were responsible for the greater efficiency of β-oxidation relative to that ofE. coli.

Bibliography

Iram, S. H., & Cronan, J. E. (2006). The Î²-Oxidation Systems ofEscherichia coliandSalmonella entericaAre Not Functionally Equivalent. Journal of Bacteriology, 188(2), 599â608.

Authors 2

Surtaj Hussain Iram (first)
John E. Cronan (additional)

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Dates

Type	When
Created	19 years, 8 months ago (Dec. 29, 2005, 3:12 p.m.)
Deposited	1 year, 7 months ago (Feb. 1, 2024, 6:57 p.m.)
Indexed	6 days, 23 hours ago (Aug. 30, 2025, 12:54 p.m.)
Issued	19 years, 7 months ago (Jan. 15, 2006)
Published	19 years, 7 months ago (Jan. 15, 2006)
Published Print	19 years, 7 months ago (Jan. 15, 2006)

Funders 0

None

BibTeX

@article{Iram_2006, title={The β-Oxidation Systems ofEscherichia coliandSalmonella entericaAre Not Functionally Equivalent}, volume={188}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.188.2.599-608.2006}, DOI={10.1128/jb.188.2.599-608.2006}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Iram, Surtaj Hussain and Cronan, John E.}, year={2006}, month=jan, pages={599–608} }

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