High-Affinity Vanadate Transport System in the CyanobacteriumAnabaena variabilisATCC 29413
10.1128/jb.188.2.464-468.2006
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

ABSTRACTHigh-affinity vanadate transport systems have not heretofore been identified in any organism.Anabaena variabilis, which can fix nitrogen by using an alternative V-dependent nitrogenase, transported vanadate well. The concentration of vanadate giving half-maximum V-nitrogenase activity when added to V-starved cells was about 3 × 10−9M. The genes for an ABC-type vanadate transport system,vupABC, were found inA. variabilisabout 5 kb from the major cluster of genes encoding the V-nitrogenase, and like those genes, thevupABCgenes were repressed by molybdate; however, unlike the V-nitrogenase genes the vanadate transport genes were expressed in vegetative cells. AvupBmutant failed to grow by using V-nitrogenase unless high levels of vanadate were provided, suggesting that there was also a low-affinity vanadate transport system that functioned in thevupBmutant. ThevupABCgenes belong to a family of putative metal transport genes that include only one other characterized transport system, the tungstate transport genes ofEubacterium acidaminophilum. Similar genes are not present in the complete genomes of other bacterial strains that have a V-nitrogenase, includingAzotobacter vinelandii,Rhodopseudomonas palustris, andMethanosarcina barkeri.

Bibliography

Pratte, B. S., & Thiel, T. (2006). High-Affinity Vanadate Transport System in the CyanobacteriumAnabaena variabilisATCC 29413. Journal of Bacteriology, 188(2), 464–468.

Authors 2
  1. Brenda S. Pratte (first)
  2. Teresa Thiel (additional)
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Dates
Type When
Created 19 years, 8 months ago (Dec. 29, 2005, 3:12 p.m.)
Deposited 1 year, 7 months ago (Feb. 1, 2024, 6:57 p.m.)
Indexed 1 month, 3 weeks ago (July 14, 2025, 11:32 p.m.)
Issued 19 years, 7 months ago (Jan. 15, 2006)
Published 19 years, 7 months ago (Jan. 15, 2006)
Published Print 19 years, 7 months ago (Jan. 15, 2006)
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@article{Pratte_2006, title={High-Affinity Vanadate Transport System in the CyanobacteriumAnabaena variabilisATCC 29413}, volume={188}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.188.2.464-468.2006}, DOI={10.1128/jb.188.2.464-468.2006}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Pratte, Brenda S. and Thiel, Teresa}, year={2006}, month=jan, pages={464–468} }