Evidence that the Essential Response Regulator YycF inStreptococcus pneumoniaeModulates Expression of Fatty Acid Biosynthesis Genes and Alters Membrane Composition
10.1128/jb.187.7.2357-2367.2005
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

ABSTRACTThe YycFG two-component system, originally identified inBacillus subtilis, is highly conserved among gram-positive bacteria with low G+C contents. InStreptococcus pneumoniae, the YycF response regulator has been reported to be essential for cell growth, but the signal to which it responds and the gene members of the regulon remain unclear. In order to investigate the role of YycFG inS. pneumoniae, we increased the expression ofyycFby using a maltose-inducible vector and analyzed the genome-wide effects on transcription and protein expression during the course ofyycFexpression. The induction ofyycFexpression increased histidine kinaseyycGtranscript levels, suggesting an autoregulation of theyycFGoperon. Evidence from both proteomic and microarray transcriptome studies as well as analyses of membrane fatty acid composition indicated that YycFG is involved in the regulation of fatty acid biosynthesis pathways and in determining fatty acid chain lengths in membrane lipids. In agreement with recent transcriptome data on pneumococcal cells depleted of YycFG, we also identified several other potential members of the YycFG regulon that are required for virulence and cell wall biosynthesis and metabolism.

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Mohedano, M. L., Overweg, K., de la Fuente, A., Reuter, M., Altabe, S., Mulholland, F., de Mendoza, D., López, P., & Wells, J. M. (2005). Evidence that the Essential Response Regulator YycF inStreptococcus pneumoniaeModulates Expression of Fatty Acid Biosynthesis Genes and Alters Membrane Composition. Journal of Bacteriology, 187(7), 2357–2367.

Authors 9
  1. M. Luz Mohedano (first)
  2. Karin Overweg (additional)
  3. Alicia de la Fuente (additional)
  4. Mark Reuter (additional)
  5. Silvia Altabe (additional)
  6. Francis Mulholland (additional)
  7. Diego de Mendoza (additional)
  8. Paloma López (additional)
  9. Jerry M. Wells (additional)
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Dates
Type When
Created 20 years, 5 months ago (March 17, 2005, 4:41 p.m.)
Deposited 1 year, 7 months ago (Jan. 23, 2024, 10:15 p.m.)
Indexed 2 months, 1 week ago (June 27, 2025, 10:03 a.m.)
Issued 20 years, 5 months ago (April 1, 2005)
Published 20 years, 5 months ago (April 1, 2005)
Published Print 20 years, 5 months ago (April 1, 2005)
Funders 0

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@article{Mohedano_2005, title={Evidence that the Essential Response Regulator YycF inStreptococcus pneumoniaeModulates Expression of Fatty Acid Biosynthesis Genes and Alters Membrane Composition}, volume={187}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.187.7.2357-2367.2005}, DOI={10.1128/jb.187.7.2357-2367.2005}, number={7}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Mohedano, M. Luz and Overweg, Karin and de la Fuente, Alicia and Reuter, Mark and Altabe, Silvia and Mulholland, Francis and de Mendoza, Diego and López, Paloma and Wells, Jerry M.}, year={2005}, month=apr, pages={2357–2367} }