Strong Decrease in Invasive Ability and Outer Membrane Vesicle Release in Crohn's Disease-Associated Adherent-Invasive Escherichia coli Strain LF82 with the yfgL Gene Deleted
10.1128/jb.187.7.2286-2296.2005
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

ABSTRACT Adherent-invasive Escherichia coli strain LF82 recovered from a chronic lesion of a patient with Crohn's disease is able to invade cultured intestinal epithelial cells. Three mutants with impaired ability to invade epithelial cells had the Tn 5phoA transposon inserted in the yfgL gene encoding the YfgL lipoprotein. A yfgL - negative isogenic mutant showed a marked decrease both in its ability to invade Intestine-407 cells and in the amount of the outer membrane proteins OmpA and OmpC in the culture supernatant, as shown by analysis of the culture supernatant protein contents by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization-time of flight mass spectrometry. Transcomplementation of the LF82-Δ yfgL isogenic mutant with the cloned yfgL gene restored invasion ability and outer membrane protein release in the culture supernatant. The outer membrane proteins in the culture supernatant of strain LF82 resulted from the formation of vesicles. This was shown by Western blot analysis of periplasmic and outer membrane fraction markers typically found in outer membrane vesicles and by transmission electron microscopic analysis of ultracentrifuged cell-free LF82 supernatant pellets, indicating the presence of vesicles with a bilayered structure surrounding a central electron-dense core. Thus, deletion of the yfgL gene in strain LF82 resulted in a decreased ability to invade intestinal epithelial cells and a decreased release of outer membrane vesicles.

Bibliography

Rolhion, N., Barnich, N., Claret, L., & Darfeuille-Michaud, A. (2005). Strong Decrease in Invasive Ability and Outer Membrane Vesicle Release in Crohn’s Disease-Associated Adherent-Invasive Escherichia coli Strain LF82 with the yfgL Gene Deleted. Journal of Bacteriology, 187(7), 2286–2296.

Authors 4
  1. Nathalie Rolhion (first)
  2. Nicolas Barnich (additional)
  3. Laurent Claret (additional)
  4. Arlette Darfeuille-Michaud (additional)
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Dates
Type When
Created 20 years, 5 months ago (March 17, 2005, 4:41 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:26 p.m.)
Indexed 2 months, 3 weeks ago (June 6, 2025, 8:05 a.m.)
Issued 20 years, 5 months ago (April 1, 2005)
Published 20 years, 5 months ago (April 1, 2005)
Published Print 20 years, 5 months ago (April 1, 2005)
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@article{Rolhion_2005, title={Strong Decrease in Invasive Ability and Outer Membrane Vesicle Release in Crohn’s Disease-Associated Adherent-Invasive Escherichia coli Strain LF82 with the yfgL Gene Deleted}, volume={187}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.187.7.2286-2296.2005}, DOI={10.1128/jb.187.7.2286-2296.2005}, number={7}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Rolhion, Nathalie and Barnich, Nicolas and Claret, Laurent and Darfeuille-Michaud, Arlette}, year={2005}, month=apr, pages={2286–2296} }