DOI: 10.1128/jb.187.7.2233-2243.2005. Interaction Network among<i>Escherichia coli</i>Membrane Proteins Involved in Cell Division as Revealed by Bacterial Two-Hybrid Analysis

Interaction Network amongEscherichia coliMembrane Proteins Involved in Cell Division as Revealed by Bacterial Two-Hybrid Analysis

10.1128/jb.187.7.2233-2243.2005

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACTFormation of theEscherichia colidivision septum is catalyzed by a number of essential proteins (named Fts) that assemble into a ring-like structure at the future division site. Several of these Fts proteins are intrinsic transmembrane proteins whose functions are largely unknown. Although these proteins appear to be recruited to the division site in a hierarchical order, the molecular interactions underlying the assembly of the cell division machinery remain mostly unspecified. In the present study, we used a bacterial two-hybrid system based on interaction-mediated reconstitution of a cyclic AMP (cAMP) signaling cascade to unravel the molecular basis of septum assembly by analyzing the protein interaction network amongE. colicell division proteins. Our results indicate that the Fts proteins are connected to one another through multiple interactions. A deletion mapping analysis carried out with two of these proteins, FtsQ and FtsI, revealed that different regions of the polypeptides are involved in their associations with their partners. Furthermore, we showed that the association between two Fts hybrid proteins could be modulated by the coexpression of a third Fts partner. Altogether, these data suggest that the cell division machinery assembly is driven by the cooperative association among the different Fts proteins to form a dynamic multiprotein structure at the septum site. In addition, our study shows that the cAMP-based two-hybrid system is particularly appropriate for analyzing molecular interactions between membrane proteins.

Bibliography

Karimova, G., Dautin, N., & Ladant, D. (2005). Interaction Network amongEscherichia coliMembrane Proteins Involved in Cell Division as Revealed by Bacterial Two-Hybrid Analysis. Journal of Bacteriology, 187(7), 2233â2243.

Authors 3

Gouzel Karimova (first)
Nathalie Dautin (additional)
Daniel Ladant (additional)

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Dates

Type	When
Created	20 years, 5 months ago (March 17, 2005, 4:41 p.m.)
Deposited	1 year, 7 months ago (Jan. 23, 2024, 10:14 p.m.)
Indexed	1 month, 1 week ago (July 14, 2025, 11:39 p.m.)
Issued	20 years, 4 months ago (April 1, 2005)
Published	20 years, 4 months ago (April 1, 2005)
Published Print	20 years, 4 months ago (April 1, 2005)

Funders 0

None

BibTeX

@article{Karimova_2005, title={Interaction Network amongEscherichia coliMembrane Proteins Involved in Cell Division as Revealed by Bacterial Two-Hybrid Analysis}, volume={187}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.187.7.2233-2243.2005}, DOI={10.1128/jb.187.7.2233-2243.2005}, number={7}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Karimova, Gouzel and Dautin, Nathalie and Ladant, Daniel}, year={2005}, month=apr, pages={2233–2243} }

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