DOI: 10.1128/jb.187.5.1695-1701.2005. A Chimeric N-Terminal Escherichia coli -C-Terminal Rhodobacter sphaeroides FliG Rotor Protein Supports Bidirectional E. coli Flagellar Rotation and Chemotaxis

A Chimeric N-Terminal Escherichia coli -C-Terminal Rhodobacter sphaeroides FliG Rotor Protein Supports Bidirectional E. coli Flagellar Rotation and Chemotaxis

10.1128/jb.187.5.1695-1701.2005

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT Flagellate bacteria such as Escherichia coli and Salmonella enterica serovar Typhimurium typically express 5 to 12 flagellar filaments over their cell surface that rotate in clockwise (CW) and counterclockwise directions. These bacteria modulate their swimming direction towards favorable environments by biasing the direction of flagellar rotation in response to various stimuli. In contrast, Rhodobacter sphaeroides expresses a single subpolar flagellum that rotates only CW and responds tactically by a series of biased stops and starts. Rotor protein FliG transiently links the MotAB stators to the rotor, to power rotation and also has an essential function in flagellar export. In this study, we sought to determine whether the FliG protein confers directionality on flagellar motors by testing the functional properties of R. sphaeroides FliG and a chimeric FliG protein, EcRsFliG (N-terminal and central domains of E. coli FliG fused to an R. sphaeroides FliG C terminus), in an E. coli FliG null background. The EcRsFliG chimera supported flagellar synthesis and bidirectional rotation; bacteria swam and tumbled in a manner qualitatively similar to that of the wild type and showed chemotaxis to amino acids. Thus, the FliG C terminus alone does not confer the unidirectional stop-start character of the R. sphaeroides flagellar motor, and its conformation continues to support tactic, switch-protein interactions in a bidirectional motor, despite its evolutionary history in a bacterium with a unidirectional motor.

Bibliography

Morehouse, K. A., Goodfellow, I. G., & Sockett, R. E. (2005). A Chimeric N-Terminal Escherichia coli -C-Terminal Rhodobacter sphaeroides FliG Rotor Protein Supports Bidirectional E. coli Flagellar Rotation and Chemotaxis. Journal of Bacteriology, 187(5), 1695â1701.

Authors 3

Karen A. Morehouse (first)
Ian G. Goodfellow (additional)
R. Elizabeth Sockett (additional)

References 38 Referenced 7

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Dates

Type	When
Created	20 years, 6 months ago (Feb. 16, 2005, 3:59 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:26 p.m.)
Indexed	1 year, 1 month ago (July 31, 2024, 9:39 p.m.)
Issued	20 years, 6 months ago (March 1, 2005)
Published	20 years, 6 months ago (March 1, 2005)
Published Print	20 years, 6 months ago (March 1, 2005)

Funders 0

None

BibTeX

@article{Morehouse_2005, title={A Chimeric N-Terminal Escherichia coli -C-Terminal Rhodobacter sphaeroides FliG Rotor Protein Supports Bidirectional E. coli Flagellar Rotation and Chemotaxis}, volume={187}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.187.5.1695-1701.2005}, DOI={10.1128/jb.187.5.1695-1701.2005}, number={5}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Morehouse, Karen A. and Goodfellow, Ian G. and Sockett, R. Elizabeth}, year={2005}, month=mar, pages={1695–1701} }

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