DOI: 10.1128/jb.187.22.7840-7844.2005. Polar Localization of a Soluble Methyl-Accepting Protein of<i>Pseudomonas aeruginosa</i>

Polar Localization of a Soluble Methyl-Accepting Protein ofPseudomonas aeruginosa

10.1128/jb.187.22.7840-7844.2005

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACTA soluble methyl-accepting chemotaxis protein (MCP) ofPseudomonas aeruginosa, McpS, showed polar localization by immunofluorescence microscopy. Overexpression of McpS resulted in a dominant-negative effect on chemotaxis and caused a loss of polar clustering of the general MCP population. The polar localization of a soluble MCP defines a third, and unexpected, paradigm for cellular MCP localization.

Bibliography

Bardy, S. L., & Maddock, J. R. (2005). Polar Localization of a Soluble Methyl-Accepting Protein ofPseudomonas aeruginosa. Journal of Bacteriology, 187(22), 7840â7844.

Authors 2

Sonia L. Bardy (first)
Janine R. Maddock (additional)

References 31 Referenced 33

10.1111/j.1365-2958.1995.tb02413.x
10.1128/jb.176.20.6340-6348.1994
Bray, D. F., M. D. Levin, and C. J. Morton-Firth. 1998. Receptor clustering as a cellular mechanism to control sensitivity. Nature393:85-88. (10.1038/30018) / Nature (1998)
10.1128/JB.184.23.6481-6489.2002
10.1128/JB.186.19.6341-6350.2004
Ferrandez, A. C., A. C. Hawkins, D. T. Summerfield, and C. S. Harwood. 2002. Cluster II che genes from Pseudomonas aeruginosa are required for an optimal chemotactic response. J. Bacteriol.184:4373-4383. / J. Bacteriol. (2002)
Harrison, D. M., J. Skidmore, J. P. Armitage, and J. R. Maddock. 1999. Localization and environmental regulation of MCP-like proteins in Rhodobacter sphaeroides. Mol. Microbiol.31:885-892. (10.1046/j.1365-2958.1999.01226.x) / Mol. Microbiol. (1999)
Hong, C. S., A. Kuroda, T. Ikeda, N. Takiguchi, H. Ohtake, and J. Kato. 2004. The aerotaxis transducer gene aer, but not aer-2, is transcriptionally regulated by the anaerobic regulator ANR in Pseudomonas aeruginosa. J. Biosci. Bioeng.97:184-190. (10.1016/S1389-1723(04)70188-7) / J. Biosci. Bioeng. (2004)
Hong, C. S., M. Shitashiro, A. Kuroda, T. Ikeda, N. Takiguchi, H. Ohtake, and J. Kato. 2004. Chemotaxis proteins and transducers for aerotaxis in Pseudomonas aeruginosa. FEMS Microbiol. Lett.231:247-252. (10.1016/S0378-1097(04)00009-6) / FEMS Microbiol. Lett. (2004)
10.1128/JB.187.4.1533-1535.2005
Jacobs, M. A., A. Alwood, I. Thaipisuttikul, D. Spencer, E. Haugen, S. Ernst, O. Will, R. Kaul, C. Raymond, R. Levy, L. Chun-Rong, D. Guenthner, D. Bovee, M. V. Olson, and C. Manoil. 2003. Comprehensive transposon mutant library of Pseudomonas aeruginosa. Proc. Natl. Acad. Sci. USA100:14339-14344. (10.1073/pnas.2036282100) / Proc. Natl. Acad. Sci. USA (2003)
10.1128/JB.186.12.3687-3694.2004
Liu, J., and J. S. Parkinson. 1989. Role of CheW protein in coupling membrane receptors to the intracellular signaling system of bacterial chemotaxis. Proc. Natl. Acad. Sci. USA86:8703-8707. (10.1073/pnas.86.22.8703) / Proc. Natl. Acad. Sci. USA (1989)
Lybarger, S. R., and J. R. Maddock. 2000. Differences in the polar clustering of the high- and low-abundance chemoreceptors of Escherichia coli. Proc. Natl. Acad. Sci. USA97:8057-8062. (10.1073/pnas.130195397) / Proc. Natl. Acad. Sci. USA (2000)
10.1128/JB.183.11.3261-3267.2001
Lybarger, S. R., U. Nair, A. A. Lilly, G. L. Hazelbauer, and J. R. Maddock. 2005. Clustering requires modified methyl-accepting sites in low abundance but not high abundance chemoreceptors of Escherichia coli. Mol. Microbiol.56:1078-1086. (10.1111/j.1365-2958.2005.04593.x) / Mol. Microbiol. (2005)
Maddock, J. R., and L. Shapiro. 1993. Polar location of the chemoreceptor complex in the Escherichia coli cell. Science259:1717-1723. (10.1126/science.8456299) / Science (1993)
Martin, A. C., G. H. Wadhams, and J. P. Armitage. 2001. The roles of multiple CheW and CheA homologues in chemotaxis and in chemoreceptor localization in Rhodobacter sphaeroides. Mol. Microbiol.40:1261-1272. (10.1046/j.1365-2958.2001.02468.x) / Mol. Microbiol. (2001)
10.1128/jb.177.4.948-952.1995
Mattick, J. S. 2002. Type IV pili and twitching motility. Annu. Rev. Microbiol.56:289-314. (10.1146/annurev.micro.56.012302.160938) / Annu. Rev. Microbiol. (2002)
Newman, J. R., and C. Fuqua. 1999. Broad host range expression vectors that carry the L-arabinose-inducible Escherichia coli araBAD promoter and the araC regulator. Gene227:197-203. (10.1016/S0378-1119(98)00601-5) / Gene (1999)
10.1128/jb.132.2.657-665.1977
10.1128/jb.171.11.6271-6278.1989
10.1128/JB.182.4.967-973.2000
Sourjik, V., and H. C. Berg. 2000. Localization of components of the chemotaxis machinery of Escherichia coli using fluorescent protein fusions. Mol. Microbiol.37:740-751. (10.1046/j.1365-2958.2000.02044.x) / Mol. Microbiol. (2000)
10.1038/35023079
Wadhams, G. H., A. C. Martin, and J. P. Armitage. 2000. Identification and localization of a methyl-accepting chemotaxis protein in Rhodobacter sphaeroides. Mol. Microbiol.36:1222-1233. (10.1046/j.1365-2958.2000.01936.x) / Mol. Microbiol. (2000)
Wadhams, G. H., A. C. Martin, S. L. Porter, J. R. Maddock, J. C. Mantotta, H. M. King, and J. P. Armitage. 2002. TlpC, a novel chemotaxis protein in Rhodobacter sphaeroides, localizes to a discreet region in the cytoplasm. Mol. Microbiol.46:1211-1221. (10.1046/j.1365-2958.2002.03252.x) / Mol. Microbiol. (2002)
Wadhams, G. H., A. V. Warren, A. C. Martin, and J. P. Armitage. 2003. Targeting of two signal transduction pathways to different regions of the bacterial cell. Mol. Microbiol.50:763-770. (10.1046/j.1365-2958.2003.03716.x) / Mol. Microbiol. (2003)
Whitchurch, C. B., A. J. Leech, M. D. Young, D. Kennedy, J. L. Sargent, J. J. Bertrand, A. B. T. Semmler, A. S. Mellick, P. R. Martin, R. A. Alm, M. Hobbs, S. A. Beatson, B. Huang, L. Nguyen, J. C. Commollii, J. N. Engel, A. Darzins, and J. S. Mattick. 2004. Characterization of a complex chemosensory signal transduction system which controls twitching motility in Pseudomonas aeruginosa. Mol. Microbiol.52:873-893. (10.1111/j.1365-2958.2004.04026.x) / Mol. Microbiol. (2004)
Zhulin, I. B., B. L. Taylor, and R. Dixon. 1997. PAS domain S-boxes in Archaea, Bacteria and sensors for oxygen and redox. Trends Biochem. Sci.22:331-333. (10.1016/S0968-0004(97)01110-9) / Trends Biochem. Sci. (1997)

Dates

Type	When
Created	19 years, 9 months ago (Nov. 2, 2005, 5:07 p.m.)
Deposited	1 year, 6 months ago (Jan. 31, 2024, 6:20 a.m.)
Indexed	1 year ago (Aug. 24, 2024, 8:39 p.m.)
Issued	19 years, 9 months ago (Nov. 15, 2005)
Published	19 years, 9 months ago (Nov. 15, 2005)
Published Print	19 years, 9 months ago (Nov. 15, 2005)

Funders 0

None

BibTeX

@article{Bardy_2005, title={Polar Localization of a Soluble Methyl-Accepting Protein ofPseudomonas aeruginosa}, volume={187}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.187.22.7840-7844.2005}, DOI={10.1128/jb.187.22.7840-7844.2005}, number={22}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Bardy, Sonia L. and Maddock, Janine R.}, year={2005}, month=nov, pages={7840–7844} }

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