Evidence for Acquisition ofLegionellaType IV Secretion Substrates via Interdomain Horizontal Gene Transfer
10.1128/jb.187.22.7716-7726.2005
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

ABSTRACTIntracellular pathogens exploit host cell functions to create a replication niche inside eukaryotic cells. The causative agent of Legionnaires' disease, the γ-proteobacteriumLegionella pneumophila, resides and replicates within a modified vacuole of protozoan and mammalian cells.L. pneumophilatranslocates effector proteins into host cells through the Icm-Dot complex, a specialized type IVB secretion system that is required for intracellular growth. To find out if some effector proteins may have been acquired through interdomain horizontal gene transfer (HGT), we performed a bioinformatic screen that searched for eukaryotic motifs in all open reading frames of theL. pneumophilaPhiladelphia-1 genome. We found 44 uncharacterized genes with many distinct eukaryotic motifs. Most of these genes contain G+C biases compared to otherL. pneumophilagenes, supporting the theory that they were acquired through HGT. Furthermore, we found that several of them are expressed and up-regulated in stationary phase in an RpoS-dependent manner. In addition, at least seven of these gene products are translocated into host cells via the Icm-Dot complex, confirming their role in the intracellular environment. Reminiscent of the case with most Icm-Dot substrates, most of the strains containing mutations in these genes grew comparably to the parent strain intracellularly. Our findings suggest that inL. pneumophila, interdomain HGT may have been a major mechanism for the acquisition of determinants of infection.

Bibliography

de Felipe, K. S., Pampou, S., Jovanovic, O. S., Pericone, C. D., Ye, S. F., Kalachikov, S., & Shuman, H. A. (2005). Evidence for Acquisition ofLegionellaType IV Secretion Substrates via Interdomain Horizontal Gene Transfer. Journal of Bacteriology, 187(22), 7716–7726.

Authors 7
  1. Karim Suwwan de Felipe (first)
  2. Sergey Pampou (additional)
  3. Oliver S. Jovanovic (additional)
  4. Christopher D. Pericone (additional)
  5. Senna F. Ye (additional)
  6. Sergey Kalachikov (additional)
  7. Howard A. Shuman (additional)
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Dates
Type When
Created 19 years, 9 months ago (Nov. 2, 2005, 5:07 p.m.)
Deposited 1 year, 6 months ago (Jan. 31, 2024, 6:20 a.m.)
Indexed 2 days, 8 hours ago (Aug. 28, 2025, 8:30 a.m.)
Issued 19 years, 9 months ago (Nov. 15, 2005)
Published 19 years, 9 months ago (Nov. 15, 2005)
Published Print 19 years, 9 months ago (Nov. 15, 2005)
Funders 0

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@article{de_Felipe_2005, title={Evidence for Acquisition ofLegionellaType IV Secretion Substrates via Interdomain Horizontal Gene Transfer}, volume={187}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.187.22.7716-7726.2005}, DOI={10.1128/jb.187.22.7716-7726.2005}, number={22}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={de Felipe, Karim Suwwan and Pampou, Sergey and Jovanovic, Oliver S. and Pericone, Christopher D. and Ye, Senna F. and Kalachikov, Sergey and Shuman, Howard A.}, year={2005}, month=nov, pages={7716–7726} }