DOI: 10.1128/jb.187.2.687-696.2005. Biochemical Activities of the <i>absA</i> Two-Component System of <i>Streptomyces coelicolor</i>

Biochemical Activities of the absA Two-Component System of Streptomyces coelicolor

10.1128/jb.187.2.687-696.2005

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT The AbsA1 sensor kinase and its cognate response regulator AbsA2 are important regulators of antibiotic synthesis in Streptomyces coelicolor . While certain point mutations in absA1 reduce or eliminate the synthesis of several antibiotics, null mutations in these genes bring about enhanced antibiotic synthesis. We show here that AbsA1, which is unusual in sequence and structure, is both an AbsA2 kinase and an AbsA2∼P phosphatase. The half-life of AbsA2∼P in solution is 68.6 min, consistent with a role in maintaining a relatively stable state of transcriptional repression or activation. We find that mutations in the absA locus that enhance antibiotic synthesis impair AbsA2 kinase activity and that mutations that repress antibiotic synthesis impair AbsA2∼P phosphatase activity. These results support a model in which the phosphorylation state of AbsA2 is determined by the balance of the kinase and phosphatase activities of AbsA1 and where AbsA2∼P represses antibiotic biosynthetic genes either directly or indirectly.

Bibliography

Sheeler, N. L., MacMillan, S. V., & Nodwell, J. R. (2005). Biochemical Activities of the absA Two-Component System of Streptomyces coelicolor. Journal of Bacteriology, 187(2), 687â696.

Authors 3

Nancy L. Sheeler (first)
Susan V. MacMillan (additional)
Justin R. Nodwell (additional)

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Dates

Type	When
Created	20 years, 7 months ago (Jan. 3, 2005, 1:33 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:24 p.m.)
Indexed	1 year ago (Aug. 10, 2024, 11:15 a.m.)
Issued	20 years, 7 months ago (Jan. 15, 2005)
Published	20 years, 7 months ago (Jan. 15, 2005)
Published Print	20 years, 7 months ago (Jan. 15, 2005)

Funders 0

None

BibTeX

@article{Sheeler_2005, title={Biochemical Activities of the absA Two-Component System of Streptomyces coelicolor}, volume={187}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.187.2.687-696.2005}, DOI={10.1128/jb.187.2.687-696.2005}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Sheeler, Nancy L. and MacMillan, Susan V. and Nodwell, Justin R.}, year={2005}, month=jan, pages={687–696} }

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