DOI: 10.1128/jb.187.15.5259-5266.2005. Identification of an <i>Escherichia coli</i> Operon Required for Formation of the O-Antigen Capsule

Identification of an Escherichia coli Operon Required for Formation of the O-Antigen Capsule

10.1128/jb.187.15.5259-5266.2005

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT Escherichia coli produces polysaccharide capsules that, based on their mechanisms of synthesis and assembly, have been classified into four groups. The group 4 capsule (G4C) polysaccharide is frequently identical to that of the cognate lipopolysaccharide O side chain and has, therefore, also been termed the O-antigen capsule. The genes involved in the assembly of the group 1, 2, and 3 capsules have been described, but those required for G4C assembly remained obscure. We found that enteropathogenic E. coli (EPEC) produces G4C, and we identified an operon containing seven genes, ymcD , ymcC , ymcB , ymcA , yccZ , etp , and etk , which are required for formation of the capsule. The encoded proteins appear to constitute a polysaccharide secretion system. The G4C operon is absent from the genomes of enteroaggregative E. coli and uropathogenic E. coli. E. coli K-12 contains the G4C operon but does not express it, because of the presence of IS 1 at its promoter region. In contrast, EPEC, enterohemorrhagic E. coli , and Shigella species possess an intact G4C operon.

Bibliography

Peleg, A., Shifrin, Y., Ilan, O., Nadler-Yona, C., Nov, S., Koby, S., Baruch, K., Altuvia, S., Elgrably-Weiss, M., Abe, C. M., Knutton, S., Saper, M. A., & Rosenshine, I. (2005). Identification of an Escherichia coli Operon Required for Formation of the O-Antigen Capsule. Journal of Bacteriology, 187(15), 5259â5266.

Authors 13

Adi Peleg (first)
Yulia Shifrin (additional)
Ophir Ilan (additional)
Chen Nadler-Yona (additional)
Shani Nov (additional)
Simi Koby (additional)
Kobi Baruch (additional)
Shoshy Altuvia (additional)
Maya Elgrably-Weiss (additional)
Cecilia M. Abe (additional)
Stuart Knutton (additional)
Mark A. Saper (additional)
Ilan Rosenshine (additional)

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Dates

Type	When
Created	20 years, 1 month ago (July 19, 2005, 3:33 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:23 p.m.)
Indexed	4 days, 21 hours ago (Aug. 27, 2025, 11:42 a.m.)
Issued	20 years, 1 month ago (Aug. 1, 2005)
Published	20 years, 1 month ago (Aug. 1, 2005)
Published Print	20 years, 1 month ago (Aug. 1, 2005)

Funders 0

None

BibTeX

@article{Peleg_2005, title={Identification of an Escherichia coli Operon Required for Formation of the O-Antigen Capsule}, volume={187}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.187.15.5259-5266.2005}, DOI={10.1128/jb.187.15.5259-5266.2005}, number={15}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Peleg, Adi and Shifrin, Yulia and Ilan, Ophir and Nadler-Yona, Chen and Nov, Shani and Koby, Simi and Baruch, Kobi and Altuvia, Shoshy and Elgrably-Weiss, Maya and Abe, Cecilia M. and Knutton, Stuart and Saper, Mark A. and Rosenshine, Ilan}, year={2005}, month=aug, pages={5259–5266} }

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