Structural and Genetic Characterization of Glycosylation of Type a Flagellin in Pseudomonas aeruginosa
10.1128/jb.186.9.2523-2531.2004
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

ABSTRACT Type a flagellins from two strains of Pseudomonas aeruginosa , strains PAK and JJ692, were found to be glycosylated with unique glycan structures. In both cases, two sites of O-linked glycosylation were identified on each monomer, and these sites were localized to the central, surface-exposed domain of the monomer in the assembled filament. The PAK flagellin was modified with a heterogeneous glycan comprising up to 11 monosaccharide units that were O linked through a rhamnose residue to the protein backbone. The flagellin of JJ692 was less complex and had a single rhamnose substitution at each site. The role of the glycosylation island gene cluster in the production of each of these glycosyl moieties was investigated. These studies revealed that the orfA and orfN genes were required for attachment of the heterologous glycan and the proximal rhamnose residue, respectively.

Bibliography

Schirm, M., Arora, S. K., Verma, A., Vinogradov, E., Thibault, P., Ramphal, R., & Logan, S. M. (2004). Structural and Genetic Characterization of Glycosylation of Type a Flagellin in Pseudomonas aeruginosa. Journal of Bacteriology, 186(9), 2523–2531.

Authors 7
  1. M. Schirm (first)
  2. S. K. Arora (additional)
  3. A. Verma (additional)
  4. E. Vinogradov (additional)
  5. P. Thibault (additional)
  6. R. Ramphal (additional)
  7. S. M. Logan (additional)
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Dates
Type When
Created 21 years, 4 months ago (April 16, 2004, 2:16 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:20 p.m.)
Indexed 2 weeks, 5 days ago (Aug. 12, 2025, 6:23 p.m.)
Issued 21 years, 4 months ago (May 1, 2004)
Published 21 years, 4 months ago (May 1, 2004)
Published Print 21 years, 4 months ago (May 1, 2004)
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@article{Schirm_2004, title={Structural and Genetic Characterization of Glycosylation of Type a Flagellin in Pseudomonas aeruginosa}, volume={186}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.186.9.2523-2531.2004}, DOI={10.1128/jb.186.9.2523-2531.2004}, number={9}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Schirm, M. and Arora, S. K. and Verma, A. and Vinogradov, E. and Thibault, P. and Ramphal, R. and Logan, S. M.}, year={2004}, month=may, pages={2523–2531} }