DOI: 10.1128/jb.186.3.785-793.2004. A Predicted ABC Transporter, FtsEX, Is Needed for Cell Division in <i>Escherichia coli</i>

A Predicted ABC Transporter, FtsEX, Is Needed for Cell Division in Escherichia coli

10.1128/jb.186.3.785-793.2004

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT FtsE and FtsX have homology to the ABC transporter superfamily of proteins and appear to be widely conserved among bacteria. Early work implicated FtsEX in cell division in Escherichia coli , but this was subsequently challenged, in part because the division defects in ftsEX mutants are often salt remedial. Strain RG60 has an ftsE :: kan null mutation that is polar onto ftsX . RG60 is mildly filamentous when grown in standard Luria-Bertani medium (LB), which contains 1% NaCl, but upon shift to LB with no NaCl growth and division stop. We found that FtsN localizes to potential division sites, albeit poorly, in RG60 grown in LB with 1% NaCl. We also found that in wild-type E. coli both FtsE and FtsX localize to the division site. Localization of FtsX was studied in detail and appeared to require FtsZ, FtsA, and ZipA, but not the downstream division proteins FtsK, FtsQ, FtsL, and FtsI. Consistent with this, in media lacking salt, FtsA and ZipA localized independently of FtsEX, but the downstream proteins did not. Finally, in the absence of salt, cells depleted of FtsEX stopped dividing before any change in growth rate (mass increase) was apparent. We conclude that FtsEX participates directly in the process of cell division and is important for assembly or stability of the septal ring, especially in salt-free media.

Bibliography

Schmidt, K. L., Peterson, N. D., Kustusch, R. J., Wissel, M. C., Graham, B., Phillips, G. J., & Weiss, D. S. (2004). A Predicted ABC Transporter, FtsEX, Is Needed for Cell Division in Escherichia coli. Journal of Bacteriology, 186(3), 785â793.

Authors 7

Kari L. Schmidt (first)
Nicholas D. Peterson (additional)
Ryan J. Kustusch (additional)
Mark C. Wissel (additional)
Becky Graham (additional)
Gregory J. Phillips (additional)
David S. Weiss (additional)

References 40 Referenced 152

Dates

Type	When
Created	21 years, 7 months ago (Jan. 16, 2004, 2:37 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:19 p.m.)
Indexed	2 days, 7 hours ago (Aug. 31, 2025, 6:29 a.m.)
Issued	21 years, 7 months ago (Feb. 1, 2004)
Published	21 years, 7 months ago (Feb. 1, 2004)
Published Print	21 years, 7 months ago (Feb. 1, 2004)

Funders 0

None

BibTeX

@article{Schmidt_2004, title={A Predicted ABC Transporter, FtsEX, Is Needed for Cell Division in Escherichia coli}, volume={186}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.186.3.785-793.2004}, DOI={10.1128/jb.186.3.785-793.2004}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Schmidt, Kari L. and Peterson, Nicholas D. and Kustusch, Ryan J. and Wissel, Mark C. and Graham, Becky and Phillips, Gregory J. and Weiss, David S.}, year={2004}, month=feb, pages={785–793} }

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