DOI: 10.1128/jb.185.9.2802-2810.2003. Propionyl Coenzyme A Is a Common Intermediate in the 1,2-Propanediol and Propionate Catabolic Pathways Needed for Expression of the <i>prpBCDE</i> Operon during Growth of <i>Salmonella enterica</i> on 1,2-Propanediol

Propionyl Coenzyme A Is a Common Intermediate in the 1,2-Propanediol and Propionate Catabolic Pathways Needed for Expression of the prpBCDE Operon during Growth of Salmonella enterica on 1,2-Propanediol

10.1128/jb.185.9.2802-2810.2003

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT The studies reported here identify propionyl coenzyme A (propionyl-CoA) as the common intermediate in the 1,2-propanediol and propionate catabolic pathways of Salmonella enterica serovar Typhimurium LT2. Growth on 1,2-propanediol as a carbon and energy source led to the formation and excretion of propionate, whose activation to propionyl-CoA relied on the activities of the propionate kinase (PduW)/phosphotransacetylase (Pta) enzyme system and the CobB sirtuin-controlled acetyl-CoA and propionyl-CoA (Acs, PrpE) synthetases. The different affinities of these systems for propionate ensure sufficient synthesis of propionyl-CoA to support wild-type growth of S. enterica under low or high concentrations of propionate in the environment. These redundant systems of propionyl-CoA synthesis are needed because the prpE gene encoding the propionyl-CoA synthetase enzyme is part of the prpBCDE operon under the control of the PrpR regulatory protein, which needs 2-methylcitrate as a coactivator. Because the synthesis of 2-methylcitrate by PrpC (i.e., the 2-methylcitrate synthase enzyme) requires propionyl-CoA as a substrate, the level of propionyl-CoA needs to be raised by the Acs or PduW-Pta system before 2-methylcitrate can be synthesized and prpBCDE transcription can be activated.

Bibliography

Palacios, S., Starai, V. J., & Escalante-Semerena, J. C. (2003). Propionyl Coenzyme A Is a Common Intermediate in the 1,2-Propanediol and Propionate Catabolic Pathways Needed for Expression of the prpBCDE Operon during Growth of Salmonella enterica on 1,2-Propanediol. Journal of Bacteriology, 185(9), 2802â2810.

Authors 3

Sergio Palacios (first)
Vincent J. Starai (additional)
Jorge C. Escalante-Semerena (additional)

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Dates

Type	When
Created	22 years, 4 months ago (April 16, 2003, 5:03 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 2:14 p.m.)
Indexed	3 months ago (May 27, 2025, 10:58 a.m.)
Issued	22 years, 3 months ago (May 1, 2003)
Published	22 years, 3 months ago (May 1, 2003)
Published Print	22 years, 3 months ago (May 1, 2003)

Funders 0

None

BibTeX

@article{Palacios_2003, title={Propionyl Coenzyme A Is a Common Intermediate in the 1,2-Propanediol and Propionate Catabolic Pathways Needed for Expression of the prpBCDE Operon during Growth of Salmonella enterica on 1,2-Propanediol}, volume={185}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.185.9.2802-2810.2003}, DOI={10.1128/jb.185.9.2802-2810.2003}, number={9}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Palacios, Sergio and Starai, Vincent J. and Escalante-Semerena, Jorge C.}, year={2003}, month=may, pages={2802–2810} }

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