DOI: 10.1128/jb.185.21.6409-6414.2003. Analysis of Specific Binding Involved in Genomic Packaging of the Double-Stranded-RNA Bacteriophage φ6

Analysis of Specific Binding Involved in Genomic Packaging of the Double-Stranded-RNA Bacteriophage φ6

10.1128/jb.185.21.6409-6414.2003

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT The genomes of bacteriophage φ6 and its relatives are packaged through a mechanism that involves the recognition and translocation of the three different plus-strand transcripts of the segmented double-stranded-RNA genomes into preformed polyhedral structures called procapsids or inner cores. The packaging requires the hydrolysis of nucleoside triphosphates and takes place in the order segment S-segment M, segment L. Packaging is dependent upon unique sequences of about 200 nucleotides near the 5′ ends of plus-strand transcripts of the three genomic segments. It appears that P1 is the determinant of the RNA binding sites. Directed mutation of P1 was used to locate regions that are important for genomic packaging. Specific binding of RNA to the exterior of the procapsid was dependent upon ATP, and a region that showed a high level of cross-linking to phage-specific RNA was located. Antibodies to peptide sequences were prepared, and their abilities to bind to the exterior of procapsids were determined. Sites sensitive to trypsin and to factor Xa were determined as well.

Bibliography

Qiao, X., Qiao, J., & Mindich, L. (2003). Analysis of Specific Binding Involved in Genomic Packaging of the Double-Stranded-RNA Bacteriophage Ï6. Journal of Bacteriology, 185(21), 6409â6414.

Authors 3

Xueying Qiao (first)
Jian Qiao (additional)
Leonard Mindich (additional)

References 25 Referenced 23

Dates

Type	When
Created	21 years, 10 months ago (Oct. 15, 2003, 3:52 p.m.)
Deposited	4 years ago (July 29, 2021, 2:11 p.m.)
Indexed	3 months, 1 week ago (May 19, 2025, 1:24 p.m.)
Issued	21 years, 9 months ago (Nov. 1, 2003)
Published	21 years, 9 months ago (Nov. 1, 2003)
Published Print	21 years, 9 months ago (Nov. 1, 2003)

Funders 0

None

BibTeX

@article{Qiao_2003, title={Analysis of Specific Binding Involved in Genomic Packaging of the Double-Stranded-RNA Bacteriophage φ6}, volume={185}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.185.21.6409-6414.2003}, DOI={10.1128/jb.185.21.6409-6414.2003}, number={21}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Qiao, Xueying and Qiao, Jian and Mindich, Leonard}, year={2003}, month=nov, pages={6409–6414} }

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