Enhanced Expression ofS-Adenosylmethionine Synthetase Causes Overproduction of Actinorhodin inStreptomyces coelicolorA3(2)
10.1128/jb.185.2.601-609.2003
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

ABSTRACTWe found that a 46-kDa protein is highly expressed in an actinorhodin-overproducingStreptomyces coelicolorA3(2) mutant (KO-179), which exhibited a low-level resistance to streptomycin. The protein was identified asS-adenosylmethionine (SAM) synthetase, which is a product of themetKgene. Enzyme assay revealed that SAM synthetase activity in strain KO-179 was 5- to 10-fold higher than in wild-type cells. The elevation of SAM synthetase activity was found to be associated with an increase in the level of intracellular SAM. RNase protection assay revealed that themetKgene was transcribed from two distinct promoters (p1 and p2) and that enhanced expression of the MetK protein in the mutant strain KO-179 was attributed to elevated transcription frommetKp2. Strikingly, the introduction of a high-copy-number plasmid containing themetKgene into wild-type cells resulted in a precocious hyperproduction of actinorhodin. Furthermore, the addition of SAM to the culture medium induced Act biosynthesis in wild-type cells. Overexpression ofmetKstimulated the expression of the pathway-specific regulatory geneactII-ORF4, as demonstrated by the RNase protection assay. The addition of SAM also caused hyperproduction of streptomycin inStreptomyces griseus. These findings implicate the significant involvement of intracellular SAM in initiating the onset of secondary metabolism inStreptomyces.

Bibliography

Okamoto, S., Lezhava, A., Hosaka, T., Okamoto-Hosoya, Y., & Ochi, K. (2003). Enhanced Expression ofS-Adenosylmethionine Synthetase Causes Overproduction of Actinorhodin inStreptomyces coelicolorA3(2). Journal of Bacteriology, 185(2), 601–609.

Authors 5
  1. Susumu Okamoto (first)
  2. Alexander Lezhava (additional)
  3. Takeshi Hosaka (additional)
  4. Yoshiko Okamoto-Hosoya (additional)
  5. Kozo Ochi (additional)
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Dates
Type When
Created 22 years, 7 months ago (Jan. 2, 2003, 2:30 p.m.)
Deposited 1 year, 7 months ago (Jan. 8, 2024, 1:18 p.m.)
Indexed 2 weeks, 5 days ago (Aug. 6, 2025, 8:45 a.m.)
Issued 22 years, 7 months ago (Jan. 15, 2003)
Published 22 years, 7 months ago (Jan. 15, 2003)
Published Print 22 years, 7 months ago (Jan. 15, 2003)
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@article{Okamoto_2003, title={Enhanced Expression ofS-Adenosylmethionine Synthetase Causes Overproduction of Actinorhodin inStreptomyces coelicolorA3(2)}, volume={185}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.185.2.601-609.2003}, DOI={10.1128/jb.185.2.601-609.2003}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Okamoto, Susumu and Lezhava, Alexander and Hosaka, Takeshi and Okamoto-Hosoya, Yoshiko and Ochi, Kozo}, year={2003}, month=jan, pages={601–609} }