DOI: 10.1128/jb.185.19.5779-5790.2003. Characterization of a Spontaneous Nonmagnetic Mutant of<i>Magnetospirillum gryphiswaldense</i>Reveals a Large Deletion Comprising a Putative Magnetosome Island

Characterization of a Spontaneous Nonmagnetic Mutant ofMagnetospirillum gryphiswaldenseReveals a Large Deletion Comprising a Putative Magnetosome Island

10.1128/jb.185.19.5779-5790.2003

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACTFrequent spontaneous loss of the magnetic phenotype was observed in stationary-phase cultures of the magnetotactic bacteriumMagnetospirillum gryphiswaldenseMSR-1. A nonmagnetic mutant, designated strain MSR-1B, was isolated and characterized. The mutant lacked any structures resembling magnetosome crystals as well as internal membrane vesicles. The growth of strain MSR-1B was impaired under all growth conditions tested, and the uptake and accumulation of iron were drastically reduced under iron-replete conditions. A large chromosomal deletion of approximately 80 kb was identified in strain MSR-1B, which comprised both the entiremamABandmamDCclusters as well as further putative operons encoding a number of magnetosome-associated proteins. A bacterial artificial chromosome clone partially covering the deleted region was isolated from the genomic library of wild-typeM. gryphiswaldense. Sequence analysis of this fragment revealed that all previously identifiedmamgenes were closely linked with genes encoding other magnetosome-associated proteins within less than 35 kb. In addition, this region was remarkably rich in insertion elements and harbored a considerable number of unknown gene families which appeared to be specific for magnetotactic bacteria. Overall, these findings suggest the existence of a putative large magnetosome island inM. gryphiswaldenseand other magnetotactic bacteria.

Bibliography

SchuÌbbe, S., Kube, M., Scheffel, A., Wawer, C., Heyen, U., Meyerdierks, A., Madkour, M. H., Mayer, F., Reinhardt, R., & SchuÌler, D. (2003). Characterization of a Spontaneous Nonmagnetic Mutant ofMagnetospirillum gryphiswaldenseReveals a Large Deletion Comprising a Putative Magnetosome Island. Journal of Bacteriology, 185(19), 5779â5790.

Authors 10

Sabrina Schübbe (first)
Michael Kube (additional)
André Scheffel (additional)
Cathrin Wawer (additional)
Udo Heyen (additional)
Anke Meyerdierks (additional)
Mohamed H. Madkour (additional)
Frank Mayer (additional)
Richard Reinhardt (additional)
Dirk Schüler (additional)

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Dates

Type	When
Created	21 years, 11 months ago (Sept. 16, 2003, 3:17 p.m.)
Deposited	2 years, 4 months ago (April 26, 2023, 6:57 p.m.)
Indexed	1 week, 6 days ago (Aug. 21, 2025, 1:43 p.m.)
Issued	21 years, 11 months ago (Oct. 1, 2003)
Published	21 years, 11 months ago (Oct. 1, 2003)
Published Print	21 years, 11 months ago (Oct. 1, 2003)

Funders 0

None

BibTeX

@article{Schu_bbe_2003, title={Characterization of a Spontaneous Nonmagnetic Mutant ofMagnetospirillum gryphiswaldenseReveals a Large Deletion Comprising a Putative Magnetosome Island}, volume={185}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.185.19.5779-5790.2003}, DOI={10.1128/jb.185.19.5779-5790.2003}, number={19}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Schübbe, Sabrina and Kube, Michael and Scheffel, André and Wawer, Cathrin and Heyen, Udo and Meyerdierks, Anke and Madkour, Mohamed H. and Mayer, Frank and Reinhardt, Richard and Schüler, Dirk}, year={2003}, month=oct, pages={5779–5790} }

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